A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation

This study explores biohydrogen production through dark fermentation, an alternative supporting sustainable hydrogen generation. Dark fermentation uses organic waste under anaerobic conditions to produce hydrogen in the absence of light. Key process parameters affecting hydrogen yield, including sub...

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Main Authors: Babak Mokhtarani, Jafar Zanganeh, Behdad Moghtaderi
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Energies
Subjects:
Online Access:https://www.mdpi.com/1996-1073/18/5/1092
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author Babak Mokhtarani
Jafar Zanganeh
Behdad Moghtaderi
author_facet Babak Mokhtarani
Jafar Zanganeh
Behdad Moghtaderi
author_sort Babak Mokhtarani
collection DOAJ
description This study explores biohydrogen production through dark fermentation, an alternative supporting sustainable hydrogen generation. Dark fermentation uses organic waste under anaerobic conditions to produce hydrogen in the absence of light. Key process parameters affecting hydrogen yield, including substrate type, microorganism selection, and fermentation conditions, were examined. Various substrates, such as organic wastes and carbohydrates, were tested, and the role of anaerobic and facultative anaerobic microorganisms in optimizing the process was analyzed. The research also focused on factors such as pH, temperature, and hydraulic retention time to enhance yields and scalability. Additionally, the study modelled the process using ASPEN Plus software 14. This simulation identifies the bottle necks of this process. Due to the lack of available data, modelling and simulation of the described processes in ASPEN Plus required certain approximations. The simulation provides insight into the key challenges that need to be addressed for hydrogen production. Future research should indeed explore current limitations, such as substrate efficiency, process scalability, and cost-effectiveness, as well as potential advancements like the genetic engineering of microbial strains and improved bioreactor designs.
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spelling doaj-art-4f207f1e8393461fa521d84186a5c8222025-08-20T02:05:09ZengMDPI AGEnergies1996-10732025-02-01185109210.3390/en18051092A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and SimulationBabak Mokhtarani0Jafar Zanganeh1Behdad Moghtaderi2Centre for Innovative Energy Technologies, The University of Newcastle, Callaghan, NSW 2308, AustraliaCentre for Innovative Energy Technologies, The University of Newcastle, Callaghan, NSW 2308, AustraliaCentre for Innovative Energy Technologies, The University of Newcastle, Callaghan, NSW 2308, AustraliaThis study explores biohydrogen production through dark fermentation, an alternative supporting sustainable hydrogen generation. Dark fermentation uses organic waste under anaerobic conditions to produce hydrogen in the absence of light. Key process parameters affecting hydrogen yield, including substrate type, microorganism selection, and fermentation conditions, were examined. Various substrates, such as organic wastes and carbohydrates, were tested, and the role of anaerobic and facultative anaerobic microorganisms in optimizing the process was analyzed. The research also focused on factors such as pH, temperature, and hydraulic retention time to enhance yields and scalability. Additionally, the study modelled the process using ASPEN Plus software 14. This simulation identifies the bottle necks of this process. Due to the lack of available data, modelling and simulation of the described processes in ASPEN Plus required certain approximations. The simulation provides insight into the key challenges that need to be addressed for hydrogen production. Future research should indeed explore current limitations, such as substrate efficiency, process scalability, and cost-effectiveness, as well as potential advancements like the genetic engineering of microbial strains and improved bioreactor designs.https://www.mdpi.com/1996-1073/18/5/1092dark fermentationhydrogen productionASPEN Plus simulationbiomassstrawglucose
spellingShingle Babak Mokhtarani
Jafar Zanganeh
Behdad Moghtaderi
A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
Energies
dark fermentation
hydrogen production
ASPEN Plus simulation
biomass
straw
glucose
title A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
title_full A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
title_fullStr A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
title_full_unstemmed A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
title_short A Review on Biohydrogen Production Through Dark Fermentation, Process Parameters and Simulation
title_sort review on biohydrogen production through dark fermentation process parameters and simulation
topic dark fermentation
hydrogen production
ASPEN Plus simulation
biomass
straw
glucose
url https://www.mdpi.com/1996-1073/18/5/1092
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