Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana)
The bigfin reef squid (Sepioteuthis lessoniana) is a cephalopod with significant potential as a farmed species. However, uncertainty surrounds the optimal feed formulation and feed strategy for S. lessoniana, especially for the early stages. This study aimed to develop a suitable moist diet formulat...
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| Language: | English |
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Elsevier
2025-10-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425004478 |
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| author | Natthawut Chanlek Jirapan Satjarak Yuzuru Ikeda Nutt Nuntapong Karun Thongprajukaew |
| author_facet | Natthawut Chanlek Jirapan Satjarak Yuzuru Ikeda Nutt Nuntapong Karun Thongprajukaew |
| author_sort | Natthawut Chanlek |
| collection | DOAJ |
| description | The bigfin reef squid (Sepioteuthis lessoniana) is a cephalopod with significant potential as a farmed species. However, uncertainty surrounds the optimal feed formulation and feed strategy for S. lessoniana, especially for the early stages. This study aimed to develop a suitable moist diet formulation to improve growth performance and feed utilization of young squid. Initially, eight moist meat-based diet formulations were screened using an in vitro digestibility technique. Four formulations were considered suitable: the meat of Indian mackerel (IM), the conventional ration of whiteleg shrimp (WS), Indian mackerel and trash fish (IT), and Indian mackerel, striped catfish and trash fish (IST). An in vivo feeding trial was conducted, using a completely randomized design with triplicate groups of eight-day-old squid (39.0 ± 0.1 mg initial weight) for two weeks. Newly hatched squid fed the IST diet showed significantly higher growth than other groups, followed by squid fed the IT diet (P < 0.05). The IST group showed the lowest feed conversion ratio but moderate protein efficiency ratio. Feed intake and survival rate, however, did not vary significantly across the groups. Proteinase-specific activity was higher in the IST group, and levels of mantle myosin and its sum with actin were significantly elevated compared to other groups. There were no significant differences in mantle RNA concentration, protein concentration, and RNA/protein ratio between IT and IST groups and the WS group. These findings suggest that a well-formulated artificial diet for newly hatched bigfin reef squid can improve their growth and feed efficiency. |
| format | Article |
| id | doaj-art-4f1ea80e9ac84df2a1d79b9a45b064b3 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-4f1ea80e9ac84df2a1d79b9a45b064b32025-08-25T04:14:34ZengElsevierAquaculture Reports2352-51342025-10-014410306110.1016/j.aqrep.2025.103061Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana)Natthawut Chanlek0Jirapan Satjarak1Yuzuru Ikeda2Nutt Nuntapong3Karun Thongprajukaew4Aquatic Science and Innovative Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, ThailandAquatic Science and Innovative Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Chemistry, Biology, and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, JapanAquatic Science and Innovative Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, ThailandDivision of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand; Center of Excellence in Agricultural and Natural Resources Biotechnology Phase 3, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, Thailand; Corresponding author at: Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.The bigfin reef squid (Sepioteuthis lessoniana) is a cephalopod with significant potential as a farmed species. However, uncertainty surrounds the optimal feed formulation and feed strategy for S. lessoniana, especially for the early stages. This study aimed to develop a suitable moist diet formulation to improve growth performance and feed utilization of young squid. Initially, eight moist meat-based diet formulations were screened using an in vitro digestibility technique. Four formulations were considered suitable: the meat of Indian mackerel (IM), the conventional ration of whiteleg shrimp (WS), Indian mackerel and trash fish (IT), and Indian mackerel, striped catfish and trash fish (IST). An in vivo feeding trial was conducted, using a completely randomized design with triplicate groups of eight-day-old squid (39.0 ± 0.1 mg initial weight) for two weeks. Newly hatched squid fed the IST diet showed significantly higher growth than other groups, followed by squid fed the IT diet (P < 0.05). The IST group showed the lowest feed conversion ratio but moderate protein efficiency ratio. Feed intake and survival rate, however, did not vary significantly across the groups. Proteinase-specific activity was higher in the IST group, and levels of mantle myosin and its sum with actin were significantly elevated compared to other groups. There were no significant differences in mantle RNA concentration, protein concentration, and RNA/protein ratio between IT and IST groups and the WS group. These findings suggest that a well-formulated artificial diet for newly hatched bigfin reef squid can improve their growth and feed efficiency.http://www.sciencedirect.com/science/article/pii/S2352513425004478Feed formulationFeed ingredientIn vitro digestibilityMoist dietS. lessoniana |
| spellingShingle | Natthawut Chanlek Jirapan Satjarak Yuzuru Ikeda Nutt Nuntapong Karun Thongprajukaew Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) Aquaculture Reports Feed formulation Feed ingredient In vitro digestibility Moist diet S. lessoniana |
| title | Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) |
| title_full | Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) |
| title_fullStr | Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) |
| title_full_unstemmed | Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) |
| title_short | Suitable formulation of moist diet for newly hatched bigfin reef squid (Sepioteuthis lessoniana) |
| title_sort | suitable formulation of moist diet for newly hatched bigfin reef squid sepioteuthis lessoniana |
| topic | Feed formulation Feed ingredient In vitro digestibility Moist diet S. lessoniana |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425004478 |
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