A systematic discussion and comparison of the construction methods of synthetic microbial community

Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial community from construction to functional verification. Here, we review the concept, characteristics,...

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Main Authors: Chenglong Li, Yanfeng Han, Xiao Zou, Xueqian Zhang, Qingsong Ran, Chunbo Dong
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Synthetic and Systems Biotechnology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24000954
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author Chenglong Li
Yanfeng Han
Xiao Zou
Xueqian Zhang
Qingsong Ran
Chunbo Dong
author_facet Chenglong Li
Yanfeng Han
Xiao Zou
Xueqian Zhang
Qingsong Ran
Chunbo Dong
author_sort Chenglong Li
collection DOAJ
description Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial community from construction to functional verification. Here, we review the concept, characteristics, history and applications of synthetic microbial community, summarizing several methods for synthetic microbial community construction, such as isolation culture, core microbiome mining, automated design, and gene editing. In addition, we also systematically summarized the design concepts, technological thresholds, and applicable scenarios of various construction methods, and highlighted their advantages and limitations. Ultimately, this review provides four efficient, detailed, easy-to-understand and -follow steps for synthetic microbial community construction, with major implications for agricultural practices, food production, and environmental governance.
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publisher KeAi Communications Co., Ltd.
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series Synthetic and Systems Biotechnology
spelling doaj-art-4f15466f95974bf69b77fb7d27a4b94d2024-12-15T06:16:04ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2024-12-0194775783A systematic discussion and comparison of the construction methods of synthetic microbial communityChenglong Li0Yanfeng Han1Xiao Zou2Xueqian Zhang3Qingsong Ran4Chunbo Dong5Institute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaInstitute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaInstitute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaInstitute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaInstitute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaCorresponding author.; Institute of Fungus Resources, Department of Ecology/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, ChinaSynthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial community from construction to functional verification. Here, we review the concept, characteristics, history and applications of synthetic microbial community, summarizing several methods for synthetic microbial community construction, such as isolation culture, core microbiome mining, automated design, and gene editing. In addition, we also systematically summarized the design concepts, technological thresholds, and applicable scenarios of various construction methods, and highlighted their advantages and limitations. Ultimately, this review provides four efficient, detailed, easy-to-understand and -follow steps for synthetic microbial community construction, with major implications for agricultural practices, food production, and environmental governance.http://www.sciencedirect.com/science/article/pii/S2405805X24000954Synthetic communityMicrobial symbiosisCore microbiomeCommunity assemblyMicrobial technology
spellingShingle Chenglong Li
Yanfeng Han
Xiao Zou
Xueqian Zhang
Qingsong Ran
Chunbo Dong
A systematic discussion and comparison of the construction methods of synthetic microbial community
Synthetic and Systems Biotechnology
Synthetic community
Microbial symbiosis
Core microbiome
Community assembly
Microbial technology
title A systematic discussion and comparison of the construction methods of synthetic microbial community
title_full A systematic discussion and comparison of the construction methods of synthetic microbial community
title_fullStr A systematic discussion and comparison of the construction methods of synthetic microbial community
title_full_unstemmed A systematic discussion and comparison of the construction methods of synthetic microbial community
title_short A systematic discussion and comparison of the construction methods of synthetic microbial community
title_sort systematic discussion and comparison of the construction methods of synthetic microbial community
topic Synthetic community
Microbial symbiosis
Core microbiome
Community assembly
Microbial technology
url http://www.sciencedirect.com/science/article/pii/S2405805X24000954
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