Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances
Nutrition intervention is an effective way to improve flesh qualities of fish. The effect of feed supplementation with glutamate (Glu) on flesh quality of gibel carp (Carassius gibelio) was investigated. In trial 1, the fish (initial weight: 37.49 ± 0.08 g) were fed two practical diets with 0 and 2%...
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Cambridge University Press
2025-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679025100098/type/journal_article |
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| author | Wanjie Cai Xing Wang Haokun Liu Luohai Hua Dong Han Xiaoming Zhu Junyan Jin Zhimin Zhang Yunxia Yang Shouqi Xie |
| author_facet | Wanjie Cai Xing Wang Haokun Liu Luohai Hua Dong Han Xiaoming Zhu Junyan Jin Zhimin Zhang Yunxia Yang Shouqi Xie |
| author_sort | Wanjie Cai |
| collection | DOAJ |
| description | Nutrition intervention is an effective way to improve flesh qualities of fish. The effect of feed supplementation with glutamate (Glu) on flesh quality of gibel carp (Carassius gibelio) was investigated. In trial 1, the fish (initial weight: 37.49 ± 0.08 g) were fed two practical diets with 0 and 2% Glu supplementation. In trial 2, the fish (37.26 ± 0.04 g) were fed two purified diets with 0 and 3% Glu supplementation. The results after feeding trials showed that dietary Glu supplementation increased the hardness and springiness of muscle, whether using practical or purified diets. Glu-supplemented diets increased the thickness and density of myofibres and collagen content between myofibres. Furthermore, Glu promoted muscle protein deposition by regulating the IGF-1-AKT-mTOR signalling pathway, and enhanced the myofibre hypertrophy by upregulating genes related to myofibre growth and development (mef2a, mef2d, myod, myf5, mlc, tpi and pax7α). The protein deposition and myofibre hypertrophy in turn improved the flesh texture. In addition, IMP content in flesh increased when supplementing Glu whether to practical or to purified diet. Metabolomics confirmed that Glu promoted the deposition of muscle-flavoured substances and purine metabolic pathway most functioned, echoed by the upregulation of key genes (ampd, ppat and adsl) in purine metabolism. The sensory test also clarified that dietary Glu improved the flesh quality by enhancing the muscle texture and flavour. Conclusively, dietary Glu supplementation can improve the flesh quality in this fish, which can further support evidence from other studies more generally that improve flesh quality of cultured fish. |
| format | Article |
| id | doaj-art-4f143aa1a99a424da16db8081abaa2a8 |
| institution | OA Journals |
| issn | 2048-6790 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj-art-4f143aa1a99a424da16db8081abaa2a82025-08-20T02:37:13ZengCambridge University PressJournal of Nutritional Science2048-67902025-01-011410.1017/jns.2025.10009Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substancesWanjie Cai0Xing Wang1Haokun Liu2https://orcid.org/0000-0003-0585-0541Luohai Hua3Dong Han4Xiaoming Zhu5Junyan Jin6https://orcid.org/0000-0002-2314-4969Zhimin Zhang7Yunxia Yang8Shouqi Xie9Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China University of Chinese Academy of Sciences, Beijing, China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China University of Chinese Academy of Sciences, Beijing, China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, China The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaNutrition intervention is an effective way to improve flesh qualities of fish. The effect of feed supplementation with glutamate (Glu) on flesh quality of gibel carp (Carassius gibelio) was investigated. In trial 1, the fish (initial weight: 37.49 ± 0.08 g) were fed two practical diets with 0 and 2% Glu supplementation. In trial 2, the fish (37.26 ± 0.04 g) were fed two purified diets with 0 and 3% Glu supplementation. The results after feeding trials showed that dietary Glu supplementation increased the hardness and springiness of muscle, whether using practical or purified diets. Glu-supplemented diets increased the thickness and density of myofibres and collagen content between myofibres. Furthermore, Glu promoted muscle protein deposition by regulating the IGF-1-AKT-mTOR signalling pathway, and enhanced the myofibre hypertrophy by upregulating genes related to myofibre growth and development (mef2a, mef2d, myod, myf5, mlc, tpi and pax7α). The protein deposition and myofibre hypertrophy in turn improved the flesh texture. In addition, IMP content in flesh increased when supplementing Glu whether to practical or to purified diet. Metabolomics confirmed that Glu promoted the deposition of muscle-flavoured substances and purine metabolic pathway most functioned, echoed by the upregulation of key genes (ampd, ppat and adsl) in purine metabolism. The sensory test also clarified that dietary Glu improved the flesh quality by enhancing the muscle texture and flavour. Conclusively, dietary Glu supplementation can improve the flesh quality in this fish, which can further support evidence from other studies more generally that improve flesh quality of cultured fish.https://www.cambridge.org/core/product/identifier/S2048679025100098/type/journal_articleFlesh qualityFlavourGlutamateMyofibreTexture |
| spellingShingle | Wanjie Cai Xing Wang Haokun Liu Luohai Hua Dong Han Xiaoming Zhu Junyan Jin Zhimin Zhang Yunxia Yang Shouqi Xie Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances Journal of Nutritional Science Flesh quality Flavour Glutamate Myofibre Texture |
| title | Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances |
| title_full | Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances |
| title_fullStr | Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances |
| title_full_unstemmed | Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances |
| title_short | Dietary supplementation with glutamate improves the flesh quality of gibel carp (Carassius gibelio) by altering muscle texture characteristics and increasing the deposition of flavour substances |
| title_sort | dietary supplementation with glutamate improves the flesh quality of gibel carp carassius gibelio by altering muscle texture characteristics and increasing the deposition of flavour substances |
| topic | Flesh quality Flavour Glutamate Myofibre Texture |
| url | https://www.cambridge.org/core/product/identifier/S2048679025100098/type/journal_article |
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