Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts
Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2020/8823209 |
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| author | Asmaa Oubihi Hanae Hosni Issmail Nounah Abdessamad Ettouil Hicham Harhar Katim Alaoui Mohammed Ouhssine Zineb Guessous |
| author_facet | Asmaa Oubihi Hanae Hosni Issmail Nounah Abdessamad Ettouil Hicham Harhar Katim Alaoui Mohammed Ouhssine Zineb Guessous |
| author_sort | Asmaa Oubihi |
| collection | DOAJ |
| description | Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats. |
| format | Article |
| id | doaj-art-4f0f0c7b01f240b2a199afa7ec601591 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-4f0f0c7b01f240b2a199afa7ec6015912025-08-20T03:36:32ZengWileyBiochemistry Research International2090-22472090-22552020-01-01202010.1155/2020/88232098823209Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. ExtractsAsmaa Oubihi0Hanae Hosni1Issmail Nounah2Abdessamad Ettouil3Hicham Harhar4Katim Alaoui5Mohammed Ouhssine6Zineb Guessous7Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, MoroccoPharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, MoroccoLaboratory of Chemistry and Organic and Bioorganic Synthesis, Faculty of Science, Geophysics, Natural Patrimony and Green Chemistry (GEOPAC) Research Center, University Mohammed V, Rabat, MoroccoLaboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, MoroccoLaboratory of Nanotechnology, Materials and Environment, Department of Chemistry, Faculty of Science, Mohammed V University in Rabat, BP. 1014, Rabat, MoroccoPharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, MoroccoLaboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, MoroccoLaboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, MoroccoThymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.http://dx.doi.org/10.1155/2020/8823209 |
| spellingShingle | Asmaa Oubihi Hanae Hosni Issmail Nounah Abdessamad Ettouil Hicham Harhar Katim Alaoui Mohammed Ouhssine Zineb Guessous Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts Biochemistry Research International |
| title | Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts |
| title_full | Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts |
| title_fullStr | Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts |
| title_full_unstemmed | Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts |
| title_short | Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts |
| title_sort | phenolic content antioxidant activity anti inflammatory potential and acute toxicity study of thymus leptobotrys murb extracts |
| url | http://dx.doi.org/10.1155/2020/8823209 |
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