NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy
Abstract Diabetic wounds pose a challenge to healing due to increased bacterial susceptibility and poor vascularization. Effective healing requires simultaneous bacterial and biofilm elimination and angiogenesis stimulation. In this study, we incorporated polyaniline (PANI) and S-Nitrosoglutathione...
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BMC
2024-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-02474-9 |
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| author | Jiajun Xie Guihua Liu Rong Chen Ding Wang Huaming Mai Qiang Zhong Yanhong Ning Jinlang Fu Zinan Tang Yixin Xu Hao Li Mingyuan Lei Hao Cheng Yuliang Huang Yang Zhang |
| author_facet | Jiajun Xie Guihua Liu Rong Chen Ding Wang Huaming Mai Qiang Zhong Yanhong Ning Jinlang Fu Zinan Tang Yixin Xu Hao Li Mingyuan Lei Hao Cheng Yuliang Huang Yang Zhang |
| author_sort | Jiajun Xie |
| collection | DOAJ |
| description | Abstract Diabetic wounds pose a challenge to healing due to increased bacterial susceptibility and poor vascularization. Effective healing requires simultaneous bacterial and biofilm elimination and angiogenesis stimulation. In this study, we incorporated polyaniline (PANI) and S-Nitrosoglutathione (GSNO) into a polyvinyl alcohol, chitosan, and hydroxypropyltrimethyl ammonium chloride chitosan (PVA/CS/HTCC) matrix, creating a versatile wound dressing membrane through electrospinning. The dressing combines the advantages of photothermal antibacterial therapy and nitric oxide gas therapy, exhibiting enduring and effective bactericidal activity and biofilm disruption against methicillin-sensitive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Escherichia coli. Furthermore, the membrane’s PTT effect and NO release exhibit significant synergistic activation, enabling a nanodetonator-like burst release of NO through NIR irradiation to disintegrate biofilms. Importantly, the nanofiber sustained a uniform release of nitric oxide, thereby catalyzing angiogenesis and advancing cellular migration. Ultimately, the employment of this membrane dressing culminated in the efficacious amelioration of diabetic-infected wounds in Sprague–Dawley rats, achieving wound closure within a concise duration of 14 days. Upon applying NIR irradiation to the PVA-CS-HTCC-PANI-GSNO nanofiber membrane, it swiftly eradicates bacteria and biofilm within 5 min, enhancing its inherent antibacterial and anti-biofilm properties through the powerful synergistic action of PTT and NO therapy. It also promotes angiogenesis, exhibits excellent biocompatibility, and is easy to use, highlighting its potential in treating diabetic wounds. Graphical Abstract |
| format | Article |
| id | doaj-art-4eeec9ff35c841aa9640f9fc56d658a9 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-4eeec9ff35c841aa9640f9fc56d658a92025-08-20T02:13:36ZengBMCJournal of Nanobiotechnology1477-31552024-05-0122112510.1186/s12951-024-02474-9NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapyJiajun Xie0Guihua Liu1Rong Chen2Ding Wang3Huaming Mai4Qiang Zhong5Yanhong Ning6Jinlang Fu7Zinan Tang8Yixin Xu9Hao Li10Mingyuan Lei11Hao Cheng12Yuliang Huang13Yang Zhang14Division of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityInstitute of Orthopaedics, Huizhou Central People’s HospitalDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityInstitute of Orthopaedics, Huizhou Central People’s HospitalDivision of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical UniversityAbstract Diabetic wounds pose a challenge to healing due to increased bacterial susceptibility and poor vascularization. Effective healing requires simultaneous bacterial and biofilm elimination and angiogenesis stimulation. In this study, we incorporated polyaniline (PANI) and S-Nitrosoglutathione (GSNO) into a polyvinyl alcohol, chitosan, and hydroxypropyltrimethyl ammonium chloride chitosan (PVA/CS/HTCC) matrix, creating a versatile wound dressing membrane through electrospinning. The dressing combines the advantages of photothermal antibacterial therapy and nitric oxide gas therapy, exhibiting enduring and effective bactericidal activity and biofilm disruption against methicillin-sensitive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Escherichia coli. Furthermore, the membrane’s PTT effect and NO release exhibit significant synergistic activation, enabling a nanodetonator-like burst release of NO through NIR irradiation to disintegrate biofilms. Importantly, the nanofiber sustained a uniform release of nitric oxide, thereby catalyzing angiogenesis and advancing cellular migration. Ultimately, the employment of this membrane dressing culminated in the efficacious amelioration of diabetic-infected wounds in Sprague–Dawley rats, achieving wound closure within a concise duration of 14 days. Upon applying NIR irradiation to the PVA-CS-HTCC-PANI-GSNO nanofiber membrane, it swiftly eradicates bacteria and biofilm within 5 min, enhancing its inherent antibacterial and anti-biofilm properties through the powerful synergistic action of PTT and NO therapy. It also promotes angiogenesis, exhibits excellent biocompatibility, and is easy to use, highlighting its potential in treating diabetic wounds. Graphical Abstracthttps://doi.org/10.1186/s12951-024-02474-9S-NitrosoglutathioneNanofiber membranePhotothermal antibacterial therapyNitric oxide shatteringMultifunctional electrospun dressingDiabetic wound healing |
| spellingShingle | Jiajun Xie Guihua Liu Rong Chen Ding Wang Huaming Mai Qiang Zhong Yanhong Ning Jinlang Fu Zinan Tang Yixin Xu Hao Li Mingyuan Lei Hao Cheng Yuliang Huang Yang Zhang NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy Journal of Nanobiotechnology S-Nitrosoglutathione Nanofiber membrane Photothermal antibacterial therapy Nitric oxide shattering Multifunctional electrospun dressing Diabetic wound healing |
| title | NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy |
| title_full | NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy |
| title_fullStr | NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy |
| title_full_unstemmed | NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy |
| title_short | NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy |
| title_sort | nir activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide based gas therapy |
| topic | S-Nitrosoglutathione Nanofiber membrane Photothermal antibacterial therapy Nitric oxide shattering Multifunctional electrospun dressing Diabetic wound healing |
| url | https://doi.org/10.1186/s12951-024-02474-9 |
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