Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species
Capillary electrophoresis single-strand conformational polymorphism (CE-SSCP) was explored as a fast and inexpensive method to differentiate both prokaryotic (blue-green) and eukaryotic (green and brown) algae. A selection of two blue-green algae (Nostoc muscorum and Anabaena inaequalis), five green...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/272964 |
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| author | Alice Jernigan Christa Hestekin |
| author_facet | Alice Jernigan Christa Hestekin |
| author_sort | Alice Jernigan |
| collection | DOAJ |
| description | Capillary electrophoresis single-strand conformational polymorphism (CE-SSCP) was explored as a fast and inexpensive method to differentiate both prokaryotic (blue-green) and eukaryotic (green and brown) algae. A selection of two blue-green algae (Nostoc muscorum and Anabaena inaequalis), five green algae (Chlorella vulgaris, Oedogonium foveolatum, Mougeotia sp., Scenedesmus quadricauda, and Ulothrix fimbriata), and one brown algae (Ectocarpus sp.) were examined and CE-SSCP electropherogram “fingerprints” were compared to each other for two variable regions of either the 16S or 18S rDNA gene. The electropherogram patterns were remarkably stable and consistent for each particular species. The patterns were unique to each species, although some common features were observed between the different types of algae. CE-SSCP could be a useful method for monitoring changes in an algae species over time as potential shifts in species occurred. |
| format | Article |
| id | doaj-art-4eebb7c6f8924cc5be9a2efb37003739 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-4eebb7c6f8924cc5be9a2efb370037392025-08-20T02:05:35ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/272964272964Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal SpeciesAlice Jernigan0Christa Hestekin1Department of Chemical Engineering, University of Arkansas, Fayetteville, AR 72701, USADepartment of Chemical Engineering, University of Arkansas, Fayetteville, AR 72701, USACapillary electrophoresis single-strand conformational polymorphism (CE-SSCP) was explored as a fast and inexpensive method to differentiate both prokaryotic (blue-green) and eukaryotic (green and brown) algae. A selection of two blue-green algae (Nostoc muscorum and Anabaena inaequalis), five green algae (Chlorella vulgaris, Oedogonium foveolatum, Mougeotia sp., Scenedesmus quadricauda, and Ulothrix fimbriata), and one brown algae (Ectocarpus sp.) were examined and CE-SSCP electropherogram “fingerprints” were compared to each other for two variable regions of either the 16S or 18S rDNA gene. The electropherogram patterns were remarkably stable and consistent for each particular species. The patterns were unique to each species, although some common features were observed between the different types of algae. CE-SSCP could be a useful method for monitoring changes in an algae species over time as potential shifts in species occurred.http://dx.doi.org/10.1155/2015/272964 |
| spellingShingle | Alice Jernigan Christa Hestekin Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species Journal of Analytical Methods in Chemistry |
| title | Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species |
| title_full | Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species |
| title_fullStr | Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species |
| title_full_unstemmed | Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species |
| title_short | Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species |
| title_sort | capillary electrophoresis single strand conformational polymorphisms as a method to differentiate algal species |
| url | http://dx.doi.org/10.1155/2015/272964 |
| work_keys_str_mv | AT alicejernigan capillaryelectrophoresissinglestrandconformationalpolymorphismsasamethodtodifferentiatealgalspecies AT christahestekin capillaryelectrophoresissinglestrandconformationalpolymorphismsasamethodtodifferentiatealgalspecies |