Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo.
<h4>Purpose</h4>Embryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explai...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0312241 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849324132390404096 |
|---|---|
| author | Zhaoqing Gong Yujie Wang Jiayi Tang Yang Xu Hongkai Wang Yimiao Zhang Lixin Xiong Changzheng Sun Yiyang Li Yan Yang Minhua Yao Heng Cai Zengshuo Man Siyu Xuan Yangyang Tang Ziao Zhao Jiaxin Sun Dongwei Liu Yanping Su Xinghua Xu Mingjiu Luo Hongshu Sui |
| author_facet | Zhaoqing Gong Yujie Wang Jiayi Tang Yang Xu Hongkai Wang Yimiao Zhang Lixin Xiong Changzheng Sun Yiyang Li Yan Yang Minhua Yao Heng Cai Zengshuo Man Siyu Xuan Yangyang Tang Ziao Zhao Jiaxin Sun Dongwei Liu Yanping Su Xinghua Xu Mingjiu Luo Hongshu Sui |
| author_sort | Zhaoqing Gong |
| collection | DOAJ |
| description | <h4>Purpose</h4>Embryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explain the heterogeneity in oocyte quality during IVM. However, no reports have categorized, the chromatin configuration of rat GVs or evaluated, the association between the chromatin configuration and oocytes development.<h4>Methods</h4>The GV chromatin configuration of rat oocytes was divided into seven types according to the degree of chromatin compaction: non-surrounded nucleolus (NSN), prematurely condensed NSN, partly NSN, partly surrounded nucleolus (SN-1), SN-1, condensed SN-1, and aggregated (SN-2). The chromatin configuration distribution was compared during the different stages of oocyte growth and maturation. We also analyzed the changes in the chromatin configuration at different GV stages during IVM. Moreover, the factors affecting the chromatin configuration were analyzed.<h4>Results</h4>The SN-2 configuration increased with rat oocyte growth and maturation, suggesting that SN-2 facilitates oocyte development. RNA transcription activity in rat oocyte GVs was inversely correlated with oocyte IVM.<h4>Conclusions</h4>The SN-2 chromatin configuration was related to rat oocyte growth and maturation. RNA transcription activity in rat oocytes in the GV stage was inversely correlated with oocyte maturation. |
| format | Article |
| id | doaj-art-4ed5b2e30ada4b6d8dd92bbfdf8f70b8 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4ed5b2e30ada4b6d8dd92bbfdf8f70b82025-08-20T03:48:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031224110.1371/journal.pone.0312241Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo.Zhaoqing GongYujie WangJiayi TangYang XuHongkai WangYimiao ZhangLixin XiongChangzheng SunYiyang LiYan YangMinhua YaoHeng CaiZengshuo ManSiyu XuanYangyang TangZiao ZhaoJiaxin SunDongwei LiuYanping SuXinghua XuMingjiu LuoHongshu Sui<h4>Purpose</h4>Embryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explain the heterogeneity in oocyte quality during IVM. However, no reports have categorized, the chromatin configuration of rat GVs or evaluated, the association between the chromatin configuration and oocytes development.<h4>Methods</h4>The GV chromatin configuration of rat oocytes was divided into seven types according to the degree of chromatin compaction: non-surrounded nucleolus (NSN), prematurely condensed NSN, partly NSN, partly surrounded nucleolus (SN-1), SN-1, condensed SN-1, and aggregated (SN-2). The chromatin configuration distribution was compared during the different stages of oocyte growth and maturation. We also analyzed the changes in the chromatin configuration at different GV stages during IVM. Moreover, the factors affecting the chromatin configuration were analyzed.<h4>Results</h4>The SN-2 configuration increased with rat oocyte growth and maturation, suggesting that SN-2 facilitates oocyte development. RNA transcription activity in rat oocyte GVs was inversely correlated with oocyte IVM.<h4>Conclusions</h4>The SN-2 chromatin configuration was related to rat oocyte growth and maturation. RNA transcription activity in rat oocytes in the GV stage was inversely correlated with oocyte maturation.https://doi.org/10.1371/journal.pone.0312241 |
| spellingShingle | Zhaoqing Gong Yujie Wang Jiayi Tang Yang Xu Hongkai Wang Yimiao Zhang Lixin Xiong Changzheng Sun Yiyang Li Yan Yang Minhua Yao Heng Cai Zengshuo Man Siyu Xuan Yangyang Tang Ziao Zhao Jiaxin Sun Dongwei Liu Yanping Su Xinghua Xu Mingjiu Luo Hongshu Sui Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. PLoS ONE |
| title | Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. |
| title_full | Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. |
| title_fullStr | Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. |
| title_full_unstemmed | Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. |
| title_short | Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo. |
| title_sort | relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo |
| url | https://doi.org/10.1371/journal.pone.0312241 |
| work_keys_str_mv | AT zhaoqinggong relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yujiewang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT jiayitang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yangxu relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT hongkaiwang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yimiaozhang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT lixinxiong relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT changzhengsun relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yiyangli relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yanyang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT minhuayao relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT hengcai relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT zengshuoman relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT siyuxuan relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yangyangtang relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT ziaozhao relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT jiaxinsun relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT dongweiliu relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT yanpingsu relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT xinghuaxu relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT mingjiuluo relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo AT hongshusui relationshipbetweenchromatinconfigurationandmaturationabilityofratoocytesinvitroandinvivo |