Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties

To investigate the relationship between the rhizosphere microbial community structure and lotus root texture, the biological properties, and the rhizosphere microbial composition of mealy (ML) and crunchy lotus (CL) varieties were all analyzed using traditional and high-throughput sequencing technol...

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Main Authors: Xinni Li, Qiyue Liang, Meiping Gao, Yangxiu Ou, Yifeng Hu, Wen Jiang, Huiping Jiang, Shangdong Yang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/7/1637
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author Xinni Li
Qiyue Liang
Meiping Gao
Yangxiu Ou
Yifeng Hu
Wen Jiang
Huiping Jiang
Shangdong Yang
author_facet Xinni Li
Qiyue Liang
Meiping Gao
Yangxiu Ou
Yifeng Hu
Wen Jiang
Huiping Jiang
Shangdong Yang
author_sort Xinni Li
collection DOAJ
description To investigate the relationship between the rhizosphere microbial community structure and lotus root texture, the biological properties, and the rhizosphere microbial composition of mealy (ML) and crunchy lotus (CL) varieties were all analyzed using traditional and high-throughput sequencing technologies. The results showed that the ML varieties exhibited significantly lower moisture but higher starch contents than those of CL. Meanwhile, the rhizosphere fungal richness of ML was also significantly higher than that of CL. Moreover, the relative abundances of bacterial phyla and genera, such as Nitrospirota, Bacteroidota, Proteobacteria, and <i>Bacillus</i>, alongside fungal phyla and genera, i.e., Ascomycota and <i>Emericellopsis</i>, were enriched in rhizosphere of ML compared to CL. Functional prediction also revealed that elevated nitrogen cycling, polysaccharide degradation and cellulose breakdown functions could be detected in ML, potentially driving starch accumulation and cell wall modification. These results suggest that rhizosphere microbial composition, particularly nitrogen-cycling bacteria and lignocellulose-degrading fungi, may contribute to texture formation between texture-differentiated lotus root varieties.
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institution Kabale University
issn 2076-2607
language English
publishDate 2025-07-01
publisher MDPI AG
record_format Article
series Microorganisms
spelling doaj-art-4ed4f66a42c44c59b229adc4a0acccbf2025-08-20T03:56:46ZengMDPI AGMicroorganisms2076-26072025-07-01137163710.3390/microorganisms13071637Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root VarietiesXinni Li0Qiyue Liang1Meiping Gao2Yangxiu Ou3Yifeng Hu4Wen Jiang5Huiping Jiang6Shangdong Yang7Guangxi Key Laboratory of Agro-Environment and Agro-Products Safety, National Demonstration Center for Experimental Plant Science Education Guangxi Agricultural College, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Agro-Environment and Agro-Products Safety, National Demonstration Center for Experimental Plant Science Education Guangxi Agricultural College, Guangxi University, Nanning 530004, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Agro-Environment and Agro-Products Safety, National Demonstration Center for Experimental Plant Science Education Guangxi Agricultural College, Guangxi University, Nanning 530004, ChinaTo investigate the relationship between the rhizosphere microbial community structure and lotus root texture, the biological properties, and the rhizosphere microbial composition of mealy (ML) and crunchy lotus (CL) varieties were all analyzed using traditional and high-throughput sequencing technologies. The results showed that the ML varieties exhibited significantly lower moisture but higher starch contents than those of CL. Meanwhile, the rhizosphere fungal richness of ML was also significantly higher than that of CL. Moreover, the relative abundances of bacterial phyla and genera, such as Nitrospirota, Bacteroidota, Proteobacteria, and <i>Bacillus</i>, alongside fungal phyla and genera, i.e., Ascomycota and <i>Emericellopsis</i>, were enriched in rhizosphere of ML compared to CL. Functional prediction also revealed that elevated nitrogen cycling, polysaccharide degradation and cellulose breakdown functions could be detected in ML, potentially driving starch accumulation and cell wall modification. These results suggest that rhizosphere microbial composition, particularly nitrogen-cycling bacteria and lignocellulose-degrading fungi, may contribute to texture formation between texture-differentiated lotus root varieties.https://www.mdpi.com/2076-2607/13/7/1637lotus rootrhizospheremicrobial compositionspolysaccharidestexture variation
spellingShingle Xinni Li
Qiyue Liang
Meiping Gao
Yangxiu Ou
Yifeng Hu
Wen Jiang
Huiping Jiang
Shangdong Yang
Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
Microorganisms
lotus root
rhizosphere
microbial compositions
polysaccharides
texture variation
title Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
title_full Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
title_fullStr Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
title_full_unstemmed Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
title_short Comparative Analysis of Soil Microbial Community Structures in Rhizosphere of Two Texture-Differentiated Lotus Root Varieties
title_sort comparative analysis of soil microbial community structures in rhizosphere of two texture differentiated lotus root varieties
topic lotus root
rhizosphere
microbial compositions
polysaccharides
texture variation
url https://www.mdpi.com/2076-2607/13/7/1637
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