Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation
Tibetan sheep gastrointestinal microbial communities and metabolites showed adaptive differences with altitude, but we do not know which flora or metabolites may play an important role in acclimatization to the altitude environment. Therefore, we systematically analyzed the microbial structure and m...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1616555/full |
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| author | Jing Wang Jing Wang Jing Wang Jianbin Liu Jianbin Liu Tingting Guo Tingting Guo Chen Zheng Fan Wang Ting Liu Chao Yuan Chao Yuan Zengkui Lu Zengkui Lu |
| author_facet | Jing Wang Jing Wang Jing Wang Jianbin Liu Jianbin Liu Tingting Guo Tingting Guo Chen Zheng Fan Wang Ting Liu Chao Yuan Chao Yuan Zengkui Lu Zengkui Lu |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Tibetan sheep gastrointestinal microbial communities and metabolites showed adaptive differences with altitude, but we do not know which flora or metabolites may play an important role in acclimatization to the altitude environment. Therefore, we systematically analyzed the microbial structure and metabolites in the rumen and feces of Tibetan sheep at two altitudes (4,424 m and 2,364 m) using amplicon sequencing and untargeted metabolomics. The results showed that the bacterial communities differed greatly between the two groups, with high altitude Tibetan sheep having a higher forage fermentation capacity, and the abundance of some bacteria and fungi that were conducive to the decomposition of cellulose in rumen fluid increased significantly (especially Bacteroidota, Neocallimastigomycota, and Ascomycota), and the short chain fatty acids and NH3-N produced by metabolism also increased. There was also a significant increase in the abundance of Naganishia, which is prone to survive in extreme environments. In addition, the metabolite profiles in the rumen and feces of Tibetan sheep at two altitudes were also significantly different, and further correlation analysis showed that the differential bacteria in the rumen were mainly related to the products related to amino acid metabolism and lipid metabolism, and the differential bacteria in the feces were mainly correlated with some metabolites related to antibacterial, anti-inflammatory, anti-tumor and other disease treatment components. Collectively, these changes in microbiota and metabolites may have facilitated the adaptation of Tibetan sheep to the harsh plateau environment, contributing to their better survival and reproduction. This study provides a basis for research on the mechanisms of adaptation of Tibetan sheep to the plateau environment. |
| format | Article |
| id | doaj-art-4eb83b7ec3584c40a8c6f3abd04ec99c |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-4eb83b7ec3584c40a8c6f3abd04ec99c2025-08-20T02:35:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.16165551616555Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptationJing Wang0Jing Wang1Jing Wang2Jianbin Liu3Jianbin Liu4Tingting Guo5Tingting Guo6Chen Zheng7Fan Wang8Ting Liu9Chao Yuan10Chao Yuan11Zengkui Lu12Zengkui Lu13Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaSheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaSheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaSheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaSheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaKey Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaSheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou, ChinaTibetan sheep gastrointestinal microbial communities and metabolites showed adaptive differences with altitude, but we do not know which flora or metabolites may play an important role in acclimatization to the altitude environment. Therefore, we systematically analyzed the microbial structure and metabolites in the rumen and feces of Tibetan sheep at two altitudes (4,424 m and 2,364 m) using amplicon sequencing and untargeted metabolomics. The results showed that the bacterial communities differed greatly between the two groups, with high altitude Tibetan sheep having a higher forage fermentation capacity, and the abundance of some bacteria and fungi that were conducive to the decomposition of cellulose in rumen fluid increased significantly (especially Bacteroidota, Neocallimastigomycota, and Ascomycota), and the short chain fatty acids and NH3-N produced by metabolism also increased. There was also a significant increase in the abundance of Naganishia, which is prone to survive in extreme environments. In addition, the metabolite profiles in the rumen and feces of Tibetan sheep at two altitudes were also significantly different, and further correlation analysis showed that the differential bacteria in the rumen were mainly related to the products related to amino acid metabolism and lipid metabolism, and the differential bacteria in the feces were mainly correlated with some metabolites related to antibacterial, anti-inflammatory, anti-tumor and other disease treatment components. Collectively, these changes in microbiota and metabolites may have facilitated the adaptation of Tibetan sheep to the harsh plateau environment, contributing to their better survival and reproduction. This study provides a basis for research on the mechanisms of adaptation of Tibetan sheep to the plateau environment.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1616555/fullTibetan sheepaltitudesmicroorganismsmetabolitesadaptability |
| spellingShingle | Jing Wang Jing Wang Jing Wang Jianbin Liu Jianbin Liu Tingting Guo Tingting Guo Chen Zheng Fan Wang Ting Liu Chao Yuan Chao Yuan Zengkui Lu Zengkui Lu Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation Frontiers in Microbiology Tibetan sheep altitudes microorganisms metabolites adaptability |
| title | Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation |
| title_full | Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation |
| title_fullStr | Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation |
| title_full_unstemmed | Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation |
| title_short | Multi-omics insights into key microorganisms and metabolites in Tibetan sheep’s high-altitude adaptation |
| title_sort | multi omics insights into key microorganisms and metabolites in tibetan sheep s high altitude adaptation |
| topic | Tibetan sheep altitudes microorganisms metabolites adaptability |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1616555/full |
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