Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling
Abstract The production of value-added chemicals from lignin-derived aromatic compounds through bacterial catabolism has attracted attention. Sphingobium lignivorans SYK-6 can catabolize various lignin-derived aromatic compounds that can be used for this purpose. PcaK (Sl-PcaK), a member of the majo...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-08757-x |
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| author | Masaya Fujita Ryo Hirano Kota Nagayoshi Naofumi Kamimura Eiji Masai |
| author_facet | Masaya Fujita Ryo Hirano Kota Nagayoshi Naofumi Kamimura Eiji Masai |
| author_sort | Masaya Fujita |
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| description | Abstract The production of value-added chemicals from lignin-derived aromatic compounds through bacterial catabolism has attracted attention. Sphingobium lignivorans SYK-6 can catabolize various lignin-derived aromatic compounds that can be used for this purpose. PcaK (Sl-PcaK), a member of the major facilitator superfamily (MFS) transporters in SYK-6, is known to be involved in the uptake of protocatechuic acid (PCA) and vanillic acid (VA) in the inner membrane. Here, we demonstrated that Sl-PcaK also mediates the uptake of 4-hydroxybenzoic acid (HBA), syringic acid (SA), and ferulic acid (FA). Saro_0804 (Na-pcaK), with 58% amino acid sequence identity to Sl-PcaK, is present in Novosphingobium aromaticivorans DSM 12444. Na-pcaK plays a role in the uptake of PCA, HBA, VA, and SA. The increased expression of Sl-pcaK or Na-pcaK in SYK-6 cells improved the conversion rates of VA, SA, and FA 1.12-1.33-fold compared with the control strain, suggesting that these genes could serve as tools to improve the production efficiency of target metabolites. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-4eb739bd5b2f46ad92578a12e24740f42025-08-20T04:01:34ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-08757-xUptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funnelingMasaya Fujita0Ryo Hirano1Kota Nagayoshi2Naofumi Kamimura3Eiji Masai4Department of Materials Science and Bioengineering, Nagaoka University of TechnologyDepartment of Materials Science and Bioengineering, Nagaoka University of TechnologyDepartment of Materials Science and Bioengineering, Nagaoka University of TechnologyDepartment of Materials Science and Bioengineering, Nagaoka University of TechnologyDepartment of Materials Science and Bioengineering, Nagaoka University of TechnologyAbstract The production of value-added chemicals from lignin-derived aromatic compounds through bacterial catabolism has attracted attention. Sphingobium lignivorans SYK-6 can catabolize various lignin-derived aromatic compounds that can be used for this purpose. PcaK (Sl-PcaK), a member of the major facilitator superfamily (MFS) transporters in SYK-6, is known to be involved in the uptake of protocatechuic acid (PCA) and vanillic acid (VA) in the inner membrane. Here, we demonstrated that Sl-PcaK also mediates the uptake of 4-hydroxybenzoic acid (HBA), syringic acid (SA), and ferulic acid (FA). Saro_0804 (Na-pcaK), with 58% amino acid sequence identity to Sl-PcaK, is present in Novosphingobium aromaticivorans DSM 12444. Na-pcaK plays a role in the uptake of PCA, HBA, VA, and SA. The increased expression of Sl-pcaK or Na-pcaK in SYK-6 cells improved the conversion rates of VA, SA, and FA 1.12-1.33-fold compared with the control strain, suggesting that these genes could serve as tools to improve the production efficiency of target metabolites.https://doi.org/10.1038/s41598-025-08757-xLignin valorizationBacterial catabolismAromatic acidsMFS transportersABC transporters |
| spellingShingle | Masaya Fujita Ryo Hirano Kota Nagayoshi Naofumi Kamimura Eiji Masai Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling Scientific Reports Lignin valorization Bacterial catabolism Aromatic acids MFS transporters ABC transporters |
| title | Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling |
| title_full | Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling |
| title_fullStr | Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling |
| title_full_unstemmed | Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling |
| title_short | Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling |
| title_sort | uptake system of lignin derived aromatic acids in promising sphingomonadaceae strains for lignin valorization through biological funneling |
| topic | Lignin valorization Bacterial catabolism Aromatic acids MFS transporters ABC transporters |
| url | https://doi.org/10.1038/s41598-025-08757-x |
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