Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts
Our study was designed to screen the antibacterial potency of Princepia utilis leaf and seed extract and to measure their antioxidant effects, total phenol content, total flavonoid content, and total carbohydrate content. Collected samples were extracted by cold maceration. Hexane, ethyl acetate, me...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2022/3898939 |
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| author | Rakshya Bagale Srijana Acharya Akriti Gupta Pooja Chaudhary Gautam Prasad Chaudhary Jitendra Pandey |
| author_facet | Rakshya Bagale Srijana Acharya Akriti Gupta Pooja Chaudhary Gautam Prasad Chaudhary Jitendra Pandey |
| author_sort | Rakshya Bagale |
| collection | DOAJ |
| description | Our study was designed to screen the antibacterial potency of Princepia utilis leaf and seed extract and to measure their antioxidant effects, total phenol content, total flavonoid content, and total carbohydrate content. Collected samples were extracted by cold maceration. Hexane, ethyl acetate, methanol, and distilled water were used as extraction solvents. In the disc diffusion method, P. utilis ethyl acetate leaf extract was most prominent against Staphylococcus epidermis with a zone of inhibition (ZOI) of 13.83 mm. Similarly, methanolic leaf extract was most prominent against Staphylococcus aureus (ZOI-12.33 mm). Furthermore, the methanolic seed extract was most sensitive against Klebsiella pneumoniaee (ZOI-11.66 mm) Escherichia coli (ZOI-9.0 mm). The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.5 mg/mL and 0.6 mg/mL, respectively, were shown by ethyl acetate leaf extract against S. epidermis. Similarly, the highest values of MIC and MBC, i.e., 20.8 mg/mL and 33.3 mg/mL, respectively, were shown by hexane leaf extract against S. epidermidis. On the other hand, evaluation of antioxidant capacity revealed that ethyl acetate leaf extract showed the maximum antioxidant effect (IC50: 66.69 μg/mL). The total flavonoid contents of different extracts were measured in the range of 37 ± 0.74 μg QE/mg dry extract weight (methanolic seed extract) to 321.84 ± 4.82 μg QE/mg dry extract weight (hexane seed extract). Likewise, the total polyphenol content ranged from the hexane leaf extract (17.33 ± 0.642 μg GAE/mg dry extract weight) to ethyl acetate leaf extract (62.56 ± 1.284 μg GAE/mg dry extract weight). We found a variation in total carbohydrate content in the range of 23.55 ± 1.125 μg glucose/mg dry extract weight (hexane leaf extract) to 96.63 ± 2.253 μg glucose/mg dry extract weight (aqueous leaf extract). Overall, this study revealed that leaf and seed extract of P. utilis exhibited noteworthy antibacterial effects against diverse pathogenic microorganisms. |
| format | Article |
| id | doaj-art-4ea0b755cc1f4ece978b91dcd2f66552 |
| institution | Kabale University |
| issn | 1687-9694 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-4ea0b755cc1f4ece978b91dcd2f665522025-08-20T03:36:34ZengWileyJournal of Tropical Medicine1687-96942022-01-01202210.1155/2022/3898939Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed ExtractsRakshya Bagale0Srijana Acharya1Akriti Gupta2Pooja Chaudhary3Gautam Prasad Chaudhary4Jitendra Pandey5Department of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyOur study was designed to screen the antibacterial potency of Princepia utilis leaf and seed extract and to measure their antioxidant effects, total phenol content, total flavonoid content, and total carbohydrate content. Collected samples were extracted by cold maceration. Hexane, ethyl acetate, methanol, and distilled water were used as extraction solvents. In the disc diffusion method, P. utilis ethyl acetate leaf extract was most prominent against Staphylococcus epidermis with a zone of inhibition (ZOI) of 13.83 mm. Similarly, methanolic leaf extract was most prominent against Staphylococcus aureus (ZOI-12.33 mm). Furthermore, the methanolic seed extract was most sensitive against Klebsiella pneumoniaee (ZOI-11.66 mm) Escherichia coli (ZOI-9.0 mm). The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.5 mg/mL and 0.6 mg/mL, respectively, were shown by ethyl acetate leaf extract against S. epidermis. Similarly, the highest values of MIC and MBC, i.e., 20.8 mg/mL and 33.3 mg/mL, respectively, were shown by hexane leaf extract against S. epidermidis. On the other hand, evaluation of antioxidant capacity revealed that ethyl acetate leaf extract showed the maximum antioxidant effect (IC50: 66.69 μg/mL). The total flavonoid contents of different extracts were measured in the range of 37 ± 0.74 μg QE/mg dry extract weight (methanolic seed extract) to 321.84 ± 4.82 μg QE/mg dry extract weight (hexane seed extract). Likewise, the total polyphenol content ranged from the hexane leaf extract (17.33 ± 0.642 μg GAE/mg dry extract weight) to ethyl acetate leaf extract (62.56 ± 1.284 μg GAE/mg dry extract weight). We found a variation in total carbohydrate content in the range of 23.55 ± 1.125 μg glucose/mg dry extract weight (hexane leaf extract) to 96.63 ± 2.253 μg glucose/mg dry extract weight (aqueous leaf extract). Overall, this study revealed that leaf and seed extract of P. utilis exhibited noteworthy antibacterial effects against diverse pathogenic microorganisms.http://dx.doi.org/10.1155/2022/3898939 |
| spellingShingle | Rakshya Bagale Srijana Acharya Akriti Gupta Pooja Chaudhary Gautam Prasad Chaudhary Jitendra Pandey Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts Journal of Tropical Medicine |
| title | Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts |
| title_full | Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts |
| title_fullStr | Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts |
| title_full_unstemmed | Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts |
| title_short | Antibacterial and Antioxidant Activities of Prinsepia utilis Royle Leaf and Seed Extracts |
| title_sort | antibacterial and antioxidant activities of prinsepia utilis royle leaf and seed extracts |
| url | http://dx.doi.org/10.1155/2022/3898939 |
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