Protocol for a two-clone system to study signaling interactions among neuronal cells in a pre-clinical Alzheimer’s disease model

Protocol for a two-clone system to study signaling interactions among neuronal cells in a pre-clinical Alzheimer’s disease model

Summary: This protocol outlines a two-clone genetic mosaic approach in Drosophila to study cell signaling using the FLP/FRT (Flippase/Flippase recombination target) and Gal4/UAS (upstream activating sequence)/Gal80 systems. We generated a stable transgenic line expressing human Aβ42, and then we cro...

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Bibliographic Details
Main Authors: Aditi Singh, Prajakta Deshpande, Ankita Sarkar, Catherine Yeates, Anuradha Venkatakrishnan Chimata, Madhuri Kango-Singh, Amit Singh
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Developmental biology
Genetics
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003995
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