Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment.
Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated,...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004701&type=printable |
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| author | Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota |
| author_facet | Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota |
| author_sort | Leopold D Tientcheu |
| collection | DOAJ |
| description | Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb- compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1α were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-γ and TNF-α production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb- compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility. |
| format | Article |
| id | doaj-art-4e97c666c9cc457eb48530f44b0ac7a6 |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-4e97c666c9cc457eb48530f44b0ac7a62025-08-20T03:24:30ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-05-01105e000470110.1371/journal.pntd.0004701Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment.Leopold D TientcheuMariëlle C HaksSchadrac C AgblaJayne S SutherlandIfedayo M AdetifaSimon DonkorEdwin QuintenMohammed DaramyMartin AntonioBeate KampmannTom H M OttenhoffHazel M DockrellMartin O OtaEpidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb- compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1α were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-γ and TNF-α production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb- compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004701&type=printable |
| spellingShingle | Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. PLoS Neglected Tropical Diseases |
| title | Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
| title_full | Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
| title_fullStr | Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
| title_full_unstemmed | Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
| title_short | Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
| title_sort | host immune responses differ between m africanum and m tuberculosis infected patients following standard anti tuberculosis treatment |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004701&type=printable |
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