A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations
Nut kernel rancidity is a significant postharvest concern because it compromises flavor and decreases shelf-life. Roasting of nut kernels changes the chemical structure and leaves the kernel more susceptible to rancidity. This is highly dependent upon the composition of the nut kernel. Almonds conta...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2025-01-01
|
| Series: | Fruit Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/frures-0024-0012 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849698444084510720 |
|---|---|
| author | Gina M. Sideli Selina C. Wang Brian J. Allen Filipa S. Grilo Thomas M. Gradziel |
| author_facet | Gina M. Sideli Selina C. Wang Brian J. Allen Filipa S. Grilo Thomas M. Gradziel |
| author_sort | Gina M. Sideli |
| collection | DOAJ |
| description | Nut kernel rancidity is a significant postharvest concern because it compromises flavor and decreases shelf-life. Roasting of nut kernels changes the chemical structure and leaves the kernel more susceptible to rancidity. This is highly dependent upon the composition of the nut kernel. Almonds contain fatty acids and phenolic compounds which can reduce the effects of rancidity. Understanding the genetic basis of rancidity susceptibility can assist breeding efforts for superior almond varieties. In this study, a rancimat machine was used to measure the rancidity time in a panel of 270 almond trees over two harvest years. This panel represents seven almond families, common cultivars, and peach accessions. The rancimat uses a heat block and controlled air flow to rapidly accelerate the oxidation process from weeks or months to hours, which makes it feasible to measure rancidity. Marker-trait associations (MTAs) were made for both single-year and multi-year analysis using data generated from the rancimat and genotyping-by-sequence data. Stable MTAs were discovered on chromosome 2 for different models and years. Additional MTAs were found on chromosomes 1 and 8 which were in close proximity between years. Twenty-one almond trees, a subset of the original panel, that exhibited extended shelf-life from rancimat or exhibited a faster time to become rancid were further evaluation by biochemical analyses to confirm chemical composition. Three KASP marker assays were designed from these marker-trait associations and validated with a panel 52 almond genotypes for use in marker-assisted breeding. The markers developed from associated SNP on chromosomes 1 and 8 were found to be predictive of phenotype for shelf-life. |
| format | Article |
| id | doaj-art-4e92bc6ac37240cb8f4710b95fc2bfca |
| institution | DOAJ |
| issn | 2769-4615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj-art-4e92bc6ac37240cb8f4710b95fc2bfca2025-08-20T03:18:54ZengMaximum Academic PressFruit Research2769-46152025-01-01511910.48130/frures-0024-0012frures-0024-0012A novel detection method for lipid stability in almond leads to the discovery of marker-trait associationsGina M. Sideli0Selina C. Wang1Brian J. Allen2Filipa S. Grilo3Thomas M. Gradziel4Department of Plant Sciences, University of California, Davis, CA 95616, USADepartment of Food Science, University of California, Davis, CA 95616, USADepartment of Plant Sciences, University of California, Davis, CA 95616, USADepartment of Food Science, University of California, Davis, CA 95616, USADepartment of Plant Sciences, University of California, Davis, CA 95616, USANut kernel rancidity is a significant postharvest concern because it compromises flavor and decreases shelf-life. Roasting of nut kernels changes the chemical structure and leaves the kernel more susceptible to rancidity. This is highly dependent upon the composition of the nut kernel. Almonds contain fatty acids and phenolic compounds which can reduce the effects of rancidity. Understanding the genetic basis of rancidity susceptibility can assist breeding efforts for superior almond varieties. In this study, a rancimat machine was used to measure the rancidity time in a panel of 270 almond trees over two harvest years. This panel represents seven almond families, common cultivars, and peach accessions. The rancimat uses a heat block and controlled air flow to rapidly accelerate the oxidation process from weeks or months to hours, which makes it feasible to measure rancidity. Marker-trait associations (MTAs) were made for both single-year and multi-year analysis using data generated from the rancimat and genotyping-by-sequence data. Stable MTAs were discovered on chromosome 2 for different models and years. Additional MTAs were found on chromosomes 1 and 8 which were in close proximity between years. Twenty-one almond trees, a subset of the original panel, that exhibited extended shelf-life from rancimat or exhibited a faster time to become rancid were further evaluation by biochemical analyses to confirm chemical composition. Three KASP marker assays were designed from these marker-trait associations and validated with a panel 52 almond genotypes for use in marker-assisted breeding. The markers developed from associated SNP on chromosomes 1 and 8 were found to be predictive of phenotype for shelf-life.https://www.maxapress.com/article/doi/10.48130/frures-0024-0012almondlipid oxidationshelf-lifekasp markers |
| spellingShingle | Gina M. Sideli Selina C. Wang Brian J. Allen Filipa S. Grilo Thomas M. Gradziel A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations Fruit Research almond lipid oxidation shelf-life kasp markers |
| title | A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| title_full | A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| title_fullStr | A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| title_full_unstemmed | A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| title_short | A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| title_sort | novel detection method for lipid stability in almond leads to the discovery of marker trait associations |
| topic | almond lipid oxidation shelf-life kasp markers |
| url | https://www.maxapress.com/article/doi/10.48130/frures-0024-0012 |
| work_keys_str_mv | AT ginamsideli anoveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT selinacwang anoveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT brianjallen anoveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT filipasgrilo anoveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT thomasmgradziel anoveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT ginamsideli noveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT selinacwang noveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT brianjallen noveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT filipasgrilo noveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations AT thomasmgradziel noveldetectionmethodforlipidstabilityinalmondleadstothediscoveryofmarkertraitassociations |