A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme
<i>Staphylococcus aureus</i> is one of the leading causes of skin and soft tissue infections, and it is even life-threatening if it enters the bloodstream, lung or heart. In the present work, we proposed a novel colorimetric biosensor for the detection of <i>S. aureus</i> thr...
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MDPI AG
2024-09-01
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| author | Yining Fan Guanyue Gao Jinfang Zhi |
| author_facet | Yining Fan Guanyue Gao Jinfang Zhi |
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| description | <i>Staphylococcus aureus</i> is one of the leading causes of skin and soft tissue infections, and it is even life-threatening if it enters the bloodstream, lung or heart. In the present work, we proposed a novel colorimetric biosensor for the detection of <i>S. aureus</i> through hydrogen peroxide consumption. An onion-like carbon nanozyme with high peroxidase-like activity was prepared, which competed with the endogenous catalase of <i>S. aureus</i> in consuming hydrogen peroxide. This reaction was further characterized by the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine. The results showed that our approach allowed for the simple and rapid determination of <i>S. aureus</i>, with a linear range of 2 × 10<sup>4</sup> to 2 × 10<sup>7</sup> CFU/mL. Moreover, our method displayed good selectivity, with <i>Bacillus subtilis</i> and <i>Escherichia coli</i> showing negligible responses at the concentration of 2 × 10<sup>5</sup> CFU/mL. The application of the as-prepared biosensor to analyze <i>S. aureus</i> in real water samples yielded recovery rates ranging from 95% to 112%, with relative standard deviations less than 7%. The method demonstrated good accuracy and specificity, which offers a novel approach for the simple and selective detection of <i>S. aureus</i>. |
| format | Article |
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| institution | OA Journals |
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| publishDate | 2024-09-01 |
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| spelling | doaj-art-4e86427a963f465caf9ccffaa24756c12025-08-20T01:55:28ZengMDPI AGChemosensors2227-90402024-09-0112917610.3390/chemosensors12090176A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon NanozymeYining Fan0Guanyue Gao1Jinfang Zhi2Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaKey Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaKey Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China<i>Staphylococcus aureus</i> is one of the leading causes of skin and soft tissue infections, and it is even life-threatening if it enters the bloodstream, lung or heart. In the present work, we proposed a novel colorimetric biosensor for the detection of <i>S. aureus</i> through hydrogen peroxide consumption. An onion-like carbon nanozyme with high peroxidase-like activity was prepared, which competed with the endogenous catalase of <i>S. aureus</i> in consuming hydrogen peroxide. This reaction was further characterized by the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine. The results showed that our approach allowed for the simple and rapid determination of <i>S. aureus</i>, with a linear range of 2 × 10<sup>4</sup> to 2 × 10<sup>7</sup> CFU/mL. Moreover, our method displayed good selectivity, with <i>Bacillus subtilis</i> and <i>Escherichia coli</i> showing negligible responses at the concentration of 2 × 10<sup>5</sup> CFU/mL. The application of the as-prepared biosensor to analyze <i>S. aureus</i> in real water samples yielded recovery rates ranging from 95% to 112%, with relative standard deviations less than 7%. The method demonstrated good accuracy and specificity, which offers a novel approach for the simple and selective detection of <i>S. aureus</i>.https://www.mdpi.com/2227-9040/12/9/176<i>Staphylococcus aureus</i>colorimetric biosensorendogenous catalaseonion-like carbonperoxidase-like nanozyme |
| spellingShingle | Yining Fan Guanyue Gao Jinfang Zhi A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme Chemosensors <i>Staphylococcus aureus</i> colorimetric biosensor endogenous catalase onion-like carbon peroxidase-like nanozyme |
| title | A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme |
| title_full | A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme |
| title_fullStr | A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme |
| title_full_unstemmed | A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme |
| title_short | A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme |
| title_sort | novel colorimetric biosensor for the detection of catalase positive i staphylococcus aureus i based on an onion like carbon nanozyme |
| topic | <i>Staphylococcus aureus</i> colorimetric biosensor endogenous catalase onion-like carbon peroxidase-like nanozyme |
| url | https://www.mdpi.com/2227-9040/12/9/176 |
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