A Novel Colorimetric Biosensor for the Detection of Catalase-Positive <i>Staphylococcus aureus</i> Based on an Onion-like Carbon Nanozyme
<i>Staphylococcus aureus</i> is one of the leading causes of skin and soft tissue infections, and it is even life-threatening if it enters the bloodstream, lung or heart. In the present work, we proposed a novel colorimetric biosensor for the detection of <i>S. aureus</i> thr...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
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| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/12/9/176 |
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| Summary: | <i>Staphylococcus aureus</i> is one of the leading causes of skin and soft tissue infections, and it is even life-threatening if it enters the bloodstream, lung or heart. In the present work, we proposed a novel colorimetric biosensor for the detection of <i>S. aureus</i> through hydrogen peroxide consumption. An onion-like carbon nanozyme with high peroxidase-like activity was prepared, which competed with the endogenous catalase of <i>S. aureus</i> in consuming hydrogen peroxide. This reaction was further characterized by the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine. The results showed that our approach allowed for the simple and rapid determination of <i>S. aureus</i>, with a linear range of 2 × 10<sup>4</sup> to 2 × 10<sup>7</sup> CFU/mL. Moreover, our method displayed good selectivity, with <i>Bacillus subtilis</i> and <i>Escherichia coli</i> showing negligible responses at the concentration of 2 × 10<sup>5</sup> CFU/mL. The application of the as-prepared biosensor to analyze <i>S. aureus</i> in real water samples yielded recovery rates ranging from 95% to 112%, with relative standard deviations less than 7%. The method demonstrated good accuracy and specificity, which offers a novel approach for the simple and selective detection of <i>S. aureus</i>. |
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| ISSN: | 2227-9040 |