The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage
Maize, one of the most widely cultivated crops globally, is highly susceptible to mycotoxin contamination. In this study, an endophytic strain <i>Pseudomonas</i> sp. HP-1, isolated from <i>Peganum harmala</i> L., demonstrated significant biocontrol potential. The culture extr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/10/1774 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850126615455989760 |
|---|---|
| author | Marhaba Kader Liping Xu Longteng Fang Reziyamu Wufuer Minwei Zhang Nan Wei Dong Wang Zhiwei Zhang |
| author_facet | Marhaba Kader Liping Xu Longteng Fang Reziyamu Wufuer Minwei Zhang Nan Wei Dong Wang Zhiwei Zhang |
| author_sort | Marhaba Kader |
| collection | DOAJ |
| description | Maize, one of the most widely cultivated crops globally, is highly susceptible to mycotoxin contamination. In this study, an endophytic strain <i>Pseudomonas</i> sp. HP-1, isolated from <i>Peganum harmala</i> L., demonstrated significant biocontrol potential. The culture extract of <i>Pseudomonas</i> sp. HP-1 (PHE) exhibited strong antifungal activity, with inhibition zones of 40.07 ± 0.21 mm against <i>Penicillium italicum</i>, 29.71 ± 0.25 mm against <i>Aspergillus niger</i>, and 23.10 ± 0.44 mm against <i>A. flavus</i>, along with notable antibacterial activity against <i>Staphylococcus aureus</i> (22.43 ± 0.55 mm). At a concentration of 16 mg/mL, PHE almost completely inhibited the mycelial growth of <i>A. flavus</i>. The antifungal mechanism of PHE was investigated through scanning electron microscopy (SEM) and propidium iodide (PI) staining analysis, which demonstrated that antifungal activity is primarily through the disruption of cellular membrane integrity. Furthermore, PHE significantly reduced the incidence of <i>A. flavus</i> contamination in agroecological maize seeds during storage, and treated PHE showed superior antifungal efficacy compared to non-treated PHE, highlighting its potential as an effective antifungal agent for seed protection. Through one- and two-dimensional NMR and MS analyses, the primary active compound of PHE was identified as 1-phenazinecarboxylic acid. These findings indicate that PHE can be utilized as a sustainable antifungal agent for protecting maize seeds against mycotoxin-producing fungi. |
| format | Article |
| id | doaj-art-4e8224bfaa2545b99cddfad93037df70 |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-4e8224bfaa2545b99cddfad93037df702025-08-20T02:33:54ZengMDPI AGFoods2304-81582025-05-011410177410.3390/foods14101774The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed StorageMarhaba Kader0Liping Xu1Longteng Fang2Reziyamu Wufuer3Minwei Zhang4Nan Wei5Dong Wang6Zhiwei Zhang7School of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaSchool of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaSchool of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaSchool of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaInstitute of Agro-Products Storage and Processing, Xinjiang Academy of Agricultural Science, Urumqi 830091, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaSchool of Pharmaceutical Sciences, Institute of Materia Medica, Xinjiang University, Urumqi 830017, ChinaMaize, one of the most widely cultivated crops globally, is highly susceptible to mycotoxin contamination. In this study, an endophytic strain <i>Pseudomonas</i> sp. HP-1, isolated from <i>Peganum harmala</i> L., demonstrated significant biocontrol potential. The culture extract of <i>Pseudomonas</i> sp. HP-1 (PHE) exhibited strong antifungal activity, with inhibition zones of 40.07 ± 0.21 mm against <i>Penicillium italicum</i>, 29.71 ± 0.25 mm against <i>Aspergillus niger</i>, and 23.10 ± 0.44 mm against <i>A. flavus</i>, along with notable antibacterial activity against <i>Staphylococcus aureus</i> (22.43 ± 0.55 mm). At a concentration of 16 mg/mL, PHE almost completely inhibited the mycelial growth of <i>A. flavus</i>. The antifungal mechanism of PHE was investigated through scanning electron microscopy (SEM) and propidium iodide (PI) staining analysis, which demonstrated that antifungal activity is primarily through the disruption of cellular membrane integrity. Furthermore, PHE significantly reduced the incidence of <i>A. flavus</i> contamination in agroecological maize seeds during storage, and treated PHE showed superior antifungal efficacy compared to non-treated PHE, highlighting its potential as an effective antifungal agent for seed protection. Through one- and two-dimensional NMR and MS analyses, the primary active compound of PHE was identified as 1-phenazinecarboxylic acid. These findings indicate that PHE can be utilized as a sustainable antifungal agent for protecting maize seeds against mycotoxin-producing fungi.https://www.mdpi.com/2304-8158/14/10/1774<i>Pseudomonas</i>antimicrobial extractantifungal activitymaize seed1-phenazinecarboxylic acid |
| spellingShingle | Marhaba Kader Liping Xu Longteng Fang Reziyamu Wufuer Minwei Zhang Nan Wei Dong Wang Zhiwei Zhang The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage Foods <i>Pseudomonas</i> antimicrobial extract antifungal activity maize seed 1-phenazinecarboxylic acid |
| title | The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage |
| title_full | The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage |
| title_fullStr | The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage |
| title_full_unstemmed | The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage |
| title_short | The Antimicrobial Extract Derived from <i>Pseudomonas</i> sp. HP-1 for Inhibition of <i>Aspergillus flavus</i> Growth and Prolongation of Maize Seed Storage |
| title_sort | antimicrobial extract derived from i pseudomonas i sp hp 1 for inhibition of i aspergillus flavus i growth and prolongation of maize seed storage |
| topic | <i>Pseudomonas</i> antimicrobial extract antifungal activity maize seed 1-phenazinecarboxylic acid |
| url | https://www.mdpi.com/2304-8158/14/10/1774 |
| work_keys_str_mv | AT marhabakader theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT lipingxu theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT longtengfang theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT reziyamuwufuer theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT minweizhang theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT nanwei theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT dongwang theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT zhiweizhang theantimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT marhabakader antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT lipingxu antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT longtengfang antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT reziyamuwufuer antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT minweizhang antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT nanwei antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT dongwang antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage AT zhiweizhang antimicrobialextractderivedfromipseudomonasisphp1forinhibitionofiaspergillusflavusigrowthandprolongationofmaizeseedstorage |