Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes.
Chromosome movement speeds during anaphase are regulated by depolymerization of microtubules. Several models describe chromosome movement during cell division but none of them consider post-translational modifications of tubulin, even though such modifications help specify microtubules for unique ce...
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Public Library of Science (PLoS)
2024-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0311691 |
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| author | Maral Janan Jess MacPherson Arthur Forer |
| author_facet | Maral Janan Jess MacPherson Arthur Forer |
| author_sort | Maral Janan |
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| description | Chromosome movement speeds during anaphase are regulated by depolymerization of microtubules. Several models describe chromosome movement during cell division but none of them consider post-translational modifications of tubulin, even though such modifications help specify microtubules for unique cellular activities. Among these modifications, acetylation of Lysine 40 is one of the common post-translational modifications. Acetylation of microtubules greatly improves their stability, especially when subjected to cooling or drug treatment. Since kinetochore microtubules are acetylated in a variety of eukaryote cells, we wondered whether deacetylation of kinetochore microtubules was necessary in order for microtubules to be able to depolymerize during anaphase. HDAC6 (Histone Deacetylase 6) deacetylates acetylated tubulin. To study whether tubulin must be deacetylated during anaphase, we added to living cells two different HDAC6 inhibitors (Tubacin and Trichostatin A), separately, as chromosomes moved poleward in anaphase. Both HDAC6 inhibitors altered chromosome movement: chromosomes either completely stopped moving, or moved more slowly, or sometimes continued movement without speed changes. The effects of the inhibitors on chromosome movement are reversible: half-bivalents either restarted anaphase movement by themselves before washing out the inhibitor or resumed their poleward movement after the inhibitor was washed out. We suggest that kinetochore microtubules need to be deacetylated in order for normal anaphase movements to occur. |
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| language | English |
| publishDate | 2024-01-01 |
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| spelling | doaj-art-4e7bb1cbaad64df29d9c12a40f87246b2025-08-20T02:19:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031169110.1371/journal.pone.0311691Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes.Maral JananJess MacPhersonArthur ForerChromosome movement speeds during anaphase are regulated by depolymerization of microtubules. Several models describe chromosome movement during cell division but none of them consider post-translational modifications of tubulin, even though such modifications help specify microtubules for unique cellular activities. Among these modifications, acetylation of Lysine 40 is one of the common post-translational modifications. Acetylation of microtubules greatly improves their stability, especially when subjected to cooling or drug treatment. Since kinetochore microtubules are acetylated in a variety of eukaryote cells, we wondered whether deacetylation of kinetochore microtubules was necessary in order for microtubules to be able to depolymerize during anaphase. HDAC6 (Histone Deacetylase 6) deacetylates acetylated tubulin. To study whether tubulin must be deacetylated during anaphase, we added to living cells two different HDAC6 inhibitors (Tubacin and Trichostatin A), separately, as chromosomes moved poleward in anaphase. Both HDAC6 inhibitors altered chromosome movement: chromosomes either completely stopped moving, or moved more slowly, or sometimes continued movement without speed changes. The effects of the inhibitors on chromosome movement are reversible: half-bivalents either restarted anaphase movement by themselves before washing out the inhibitor or resumed their poleward movement after the inhibitor was washed out. We suggest that kinetochore microtubules need to be deacetylated in order for normal anaphase movements to occur.https://doi.org/10.1371/journal.pone.0311691 |
| spellingShingle | Maral Janan Jess MacPherson Arthur Forer Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. PLoS ONE |
| title | Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. |
| title_full | Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. |
| title_fullStr | Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. |
| title_full_unstemmed | Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. |
| title_short | Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes. |
| title_sort | blocking microtubule deacetylation inhibits anaphase chromosome movements in crane fly spermatocytes |
| url | https://doi.org/10.1371/journal.pone.0311691 |
| work_keys_str_mv | AT maraljanan blockingmicrotubuledeacetylationinhibitsanaphasechromosomemovementsincraneflyspermatocytes AT jessmacpherson blockingmicrotubuledeacetylationinhibitsanaphasechromosomemovementsincraneflyspermatocytes AT arthurforer blockingmicrotubuledeacetylationinhibitsanaphasechromosomemovementsincraneflyspermatocytes |