Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis
Abstract The exocrine pancreas is the main source of digestive enzymes which are released from secretory vesicles of acinar cells into the small intestine. Enzymes, including amylases, proteases and lipases, degrade the ingested food and thus determine the nutritional substrate for the gut microbiot...
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Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-75564-1 |
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| author | Rabea Lange Juliane Glaubitz Fabian Frost Andreas Geisz Ali A. Aghdassi F. Ulrich Weiss Matthias Sendler |
| author_facet | Rabea Lange Juliane Glaubitz Fabian Frost Andreas Geisz Ali A. Aghdassi F. Ulrich Weiss Matthias Sendler |
| author_sort | Rabea Lange |
| collection | DOAJ |
| description | Abstract The exocrine pancreas is the main source of digestive enzymes which are released from secretory vesicles of acinar cells into the small intestine. Enzymes, including amylases, proteases and lipases, degrade the ingested food and thus determine the nutritional substrate for the gut microbiota. Acute (AP) and chronic pancreatitis (CP) are associated with a transitional or progressive exocrine pancreatic dysfunction, we analysed in the present study how an experimental induction of pancreatitis in mouse models affects the colonic and duodenal microbiome composition. Evaluation by 16 S rRNA gene sequencing revealed specific microbiome changes in colonic as well as in duodenal samples in different models of AP and CP. Mild acute pancreatitis, which is associated with a transient impairment of pancreatic secretion showed only minor changes in microbial composition, comparable to the ones seen in progressive dysfunctional mouse models of CP. The strongest changes were observed in a mouse model of severe AP, which suggest a direct effect of the immune response on gut microbiome in addition to a pancreatic dysfunction. Our data indicate that highly dysbiotic microbiome changes during pancreatitis are more associated with the inflammatory reaction than with a disturbed pancreatic secretion. |
| format | Article |
| id | doaj-art-4e784e7b78f54ba38d5075d7823f12f8 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4e784e7b78f54ba38d5075d7823f12f82025-08-20T02:11:22ZengNature PortfolioScientific Reports2045-23222024-10-0114111010.1038/s41598-024-75564-1Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitisRabea Lange0Juliane Glaubitz1Fabian Frost2Andreas Geisz3Ali A. Aghdassi4F. Ulrich Weiss5Matthias Sendler6Department of Medicine A, University Medicine GreifswaldDepartment of Medicine A, University Medicine GreifswaldDepartment of Medicine A, University Medicine GreifswaldDepartment of Surgery, Boston University Chobanian and Avedisian School of MedicineDepartment of Medicine A, University Medicine GreifswaldDepartment of Medicine A, University Medicine GreifswaldDepartment of Medicine A, University Medicine GreifswaldAbstract The exocrine pancreas is the main source of digestive enzymes which are released from secretory vesicles of acinar cells into the small intestine. Enzymes, including amylases, proteases and lipases, degrade the ingested food and thus determine the nutritional substrate for the gut microbiota. Acute (AP) and chronic pancreatitis (CP) are associated with a transitional or progressive exocrine pancreatic dysfunction, we analysed in the present study how an experimental induction of pancreatitis in mouse models affects the colonic and duodenal microbiome composition. Evaluation by 16 S rRNA gene sequencing revealed specific microbiome changes in colonic as well as in duodenal samples in different models of AP and CP. Mild acute pancreatitis, which is associated with a transient impairment of pancreatic secretion showed only minor changes in microbial composition, comparable to the ones seen in progressive dysfunctional mouse models of CP. The strongest changes were observed in a mouse model of severe AP, which suggest a direct effect of the immune response on gut microbiome in addition to a pancreatic dysfunction. Our data indicate that highly dysbiotic microbiome changes during pancreatitis are more associated with the inflammatory reaction than with a disturbed pancreatic secretion.https://doi.org/10.1038/s41598-024-75564-1Acute pancreatitisChronic pancreatitisMicrobiomeInflammation |
| spellingShingle | Rabea Lange Juliane Glaubitz Fabian Frost Andreas Geisz Ali A. Aghdassi F. Ulrich Weiss Matthias Sendler Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis Scientific Reports Acute pancreatitis Chronic pancreatitis Microbiome Inflammation |
| title | Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| title_full | Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| title_fullStr | Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| title_full_unstemmed | Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| title_short | Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| title_sort | examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis |
| topic | Acute pancreatitis Chronic pancreatitis Microbiome Inflammation |
| url | https://doi.org/10.1038/s41598-024-75564-1 |
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