Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model

<b>Background:</b> Diabetes is a devastating disease that causes millions of deaths. Fermented rice bran (FRB), made by fermenting rice bran with <i>Aspergillus kawachii</i> and a mixture of lactic acid bacteria, was hypothesized to b able to improve diabetes-related symptoms...

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Main Authors: Afifah Zahra Agista, Ami Kato, Tomoko Goto, Takuya Koseki, Akira Oikawa, Yusuke Ohsaki, Michiko Yamaki, Chiu-Li Yeh, Suh-Ching Yang, Ardiansyah, Slamet Budijanto, Michio Komai, Hitoshi Shirakawa
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Language:English
Published: MDPI AG 2024-11-01
Series:Metabolites
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Online Access:https://www.mdpi.com/2218-1989/14/11/614
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author Afifah Zahra Agista
Ami Kato
Tomoko Goto
Takuya Koseki
Akira Oikawa
Yusuke Ohsaki
Michiko Yamaki
Chiu-Li Yeh
Suh-Ching Yang
Ardiansyah
Slamet Budijanto
Michio Komai
Hitoshi Shirakawa
author_facet Afifah Zahra Agista
Ami Kato
Tomoko Goto
Takuya Koseki
Akira Oikawa
Yusuke Ohsaki
Michiko Yamaki
Chiu-Li Yeh
Suh-Ching Yang
Ardiansyah
Slamet Budijanto
Michio Komai
Hitoshi Shirakawa
author_sort Afifah Zahra Agista
collection DOAJ
description <b>Background:</b> Diabetes is a devastating disease that causes millions of deaths. Fermented rice bran (FRB), made by fermenting rice bran with <i>Aspergillus kawachii</i> and a mixture of lactic acid bacteria, was hypothesized to b able to improve diabetes-related symptoms. This study aimed to investigate the effects of FRB supplementation in mitigating type 2 diabetes symptoms and identifying FRB bioactive compounds. <b>Methods</b>: In this study, KK-<i>A<sup>y</sup></i> mice (4 w.o. male) were used as a model for type 2 diabetes. Mice were divided into three different groups. The first group received a control diet, the second received a 12.5% non-fermented rice bran (RB) supplemented diet, and the last group was fed a 12.5% FRB-supplemented diet. Supplementation was done for 4 weeks. <b>Results</b>: FRB supplementation lowered the blood glucose level, OGTT, HOMA-IR, total cholesterol, liver RAGE protein, and <i>glucokinase</i> in KK-<i>A<sup>y</sup></i> mice. Metabolome analysis of RB and FRB showed that fermentation increased bioactive compounds in rice bran, such as GABA, L-theanine, and carnitine. It also increased the levels of various free amino acids while converting some amino acids such as arginine, tyrosine, and tryptophan into other metabolites. <b>Conclusions</b>: This research showed the potency of FRB supplementation as a preventive agent against type 2 diabetes.
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spelling doaj-art-4e74d04f6aca4ef5b0c48ca14ef811102025-08-20T02:05:06ZengMDPI AGMetabolites2218-19892024-11-01141161410.3390/metabo14110614Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice ModelAfifah Zahra Agista0Ami Kato1Tomoko Goto2Takuya Koseki3Akira Oikawa4Yusuke Ohsaki5Michiko Yamaki6Chiu-Li Yeh7Suh-Ching Yang8Ardiansyah9Slamet Budijanto10Michio Komai11Hitoshi Shirakawa12Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, JapanLaboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, JapanLaboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, JapanFaculty of Agriculture, Yamagata University, Tsuruoka 997-8555, JapanGraduate School of Agriculture, Kyoto University, Kyoto 606-8224, JapanLaboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, JapanDepartment of Home Economics, Division of Health and Nutrition, Tohoku Seikatsu Bunka University, Sendai 981-8585, JapanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, TaiwanDepartment of Food Technology, Universitas Bakrie, Jakarta 12920, IndonesiaFaculty of Agricultural Engineering and Technology, IPB University, Bogor 16680, IndonesiaLaboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, JapanLaboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan<b>Background:</b> Diabetes is a devastating disease that causes millions of deaths. Fermented rice bran (FRB), made by fermenting rice bran with <i>Aspergillus kawachii</i> and a mixture of lactic acid bacteria, was hypothesized to b able to improve diabetes-related symptoms. This study aimed to investigate the effects of FRB supplementation in mitigating type 2 diabetes symptoms and identifying FRB bioactive compounds. <b>Methods</b>: In this study, KK-<i>A<sup>y</sup></i> mice (4 w.o. male) were used as a model for type 2 diabetes. Mice were divided into three different groups. The first group received a control diet, the second received a 12.5% non-fermented rice bran (RB) supplemented diet, and the last group was fed a 12.5% FRB-supplemented diet. Supplementation was done for 4 weeks. <b>Results</b>: FRB supplementation lowered the blood glucose level, OGTT, HOMA-IR, total cholesterol, liver RAGE protein, and <i>glucokinase</i> in KK-<i>A<sup>y</sup></i> mice. Metabolome analysis of RB and FRB showed that fermentation increased bioactive compounds in rice bran, such as GABA, L-theanine, and carnitine. It also increased the levels of various free amino acids while converting some amino acids such as arginine, tyrosine, and tryptophan into other metabolites. <b>Conclusions</b>: This research showed the potency of FRB supplementation as a preventive agent against type 2 diabetes.https://www.mdpi.com/2218-1989/14/11/614<i>Aspergillus kawachii</i>diabetesfermented rice branlactic acid bacteria
spellingShingle Afifah Zahra Agista
Ami Kato
Tomoko Goto
Takuya Koseki
Akira Oikawa
Yusuke Ohsaki
Michiko Yamaki
Chiu-Li Yeh
Suh-Ching Yang
Ardiansyah
Slamet Budijanto
Michio Komai
Hitoshi Shirakawa
Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
Metabolites
<i>Aspergillus kawachii</i>
diabetes
fermented rice bran
lactic acid bacteria
title Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
title_full Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
title_fullStr Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
title_full_unstemmed Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
title_short Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A<sup>y</sup></i> Mice Model
title_sort fermented rice bran mitigated the syndromes of type 2 diabetes in kk i a sup y sup i mice model
topic <i>Aspergillus kawachii</i>
diabetes
fermented rice bran
lactic acid bacteria
url https://www.mdpi.com/2218-1989/14/11/614
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