CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer.
<h4>Background</h4>Recently, we reported cytoskeleton-associated protein2 (CKAP2) as a possible new prognostic breast cancer marker. However, it has not yet been applied in clinic. Therefore, clinical significance of CKAP2 was evaluated in comparison with that of Ki-67 in a cohort of bre...
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Public Library of Science (PLoS)
2017-01-01
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| author | Sung Hoon Sim Chang-Dae Bae Youngmi Kwon Hai-Li Hwang Shiv Poojan Hye-In Hong Kyungtae Kim Seo-Hee Kang Han-Seong Kim Tae-Hyun Um In Hae Park Keun Seok Lee So-Youn Jung Seeyoun Lee Han-Sung Kang Eun Sook Lee Mi-Kyung Kim Kyeong-Man Hong Jungsil Ro |
| author_facet | Sung Hoon Sim Chang-Dae Bae Youngmi Kwon Hai-Li Hwang Shiv Poojan Hye-In Hong Kyungtae Kim Seo-Hee Kang Han-Seong Kim Tae-Hyun Um In Hae Park Keun Seok Lee So-Youn Jung Seeyoun Lee Han-Sung Kang Eun Sook Lee Mi-Kyung Kim Kyeong-Man Hong Jungsil Ro |
| author_sort | Sung Hoon Sim |
| collection | DOAJ |
| description | <h4>Background</h4>Recently, we reported cytoskeleton-associated protein2 (CKAP2) as a possible new prognostic breast cancer marker. However, it has not yet been applied in clinic. Therefore, clinical significance of CKAP2 was evaluated in comparison with that of Ki-67 in a cohort of breast cancer patients, and the expression difference was analyzed in cell cycle-arrested cancer and fibroblast cells.<h4>Methods</h4>A total of 579 early breast cancer patients who underwent surgery at the National Cancer Center Hospital in Korea between 2001 and 2005 were accrued. CKAP2-positive cell count (CPCC) and Ki-67 labeling index (Ki-67LI) were evaluated by immunohistochemcal staining. The immunocytochemical staining patterns of CKAP2 and Ki-67 were analyzed in HeLa and human fibroblast cells after synchronization by double thymidine block.<h4>Results</h4>Although there was a significant correlation (R = 0.754, P < 0.001) between CPCC and Ki-67LI, only CPCC was correlated with DFS in overall population (HR, 2.029; 95% CI, 1.012-4.068; P = 0.046) and HER2-negative luminal subgroup (HR, 3.984; 95% CI, 1.350-11.762; P = 0.012) by multivariate analysis. In immunocytochemical staining, more than 50% of serum-starved or non-mitotic cell phase HeLa cells were positive for Ki-67, in comparison to the low CKAP2-positivity, which might explain the prognostic difference between CPCC and Ki-67LI.<h4>Conclusions</h4>The current study showed that CPCC but not Ki-67LI is an independent prognostic indicator in early breast cancer, more specifically in HER2-negative luminal breast cancer. The difference between two markers may be related to the lower background expression of CKAP2 in cancer cells. |
| format | Article |
| id | doaj-art-4e6c5718b3ea4af38b8abbd539e35163 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-4e6c5718b3ea4af38b8abbd539e351632025-08-20T03:12:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018210710.1371/journal.pone.0182107CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer.Sung Hoon SimChang-Dae BaeYoungmi KwonHai-Li HwangShiv PoojanHye-In HongKyungtae KimSeo-Hee KangHan-Seong KimTae-Hyun UmIn Hae ParkKeun Seok LeeSo-Youn JungSeeyoun LeeHan-Sung KangEun Sook LeeMi-Kyung KimKyeong-Man HongJungsil Ro<h4>Background</h4>Recently, we reported cytoskeleton-associated protein2 (CKAP2) as a possible new prognostic breast cancer marker. However, it has not yet been applied in clinic. Therefore, clinical significance of CKAP2 was evaluated in comparison with that of Ki-67 in a cohort of breast cancer patients, and the expression difference was analyzed in cell cycle-arrested cancer and fibroblast cells.<h4>Methods</h4>A total of 579 early breast cancer patients who underwent surgery at the National Cancer Center Hospital in Korea between 2001 and 2005 were accrued. CKAP2-positive cell count (CPCC) and Ki-67 labeling index (Ki-67LI) were evaluated by immunohistochemcal staining. The immunocytochemical staining patterns of CKAP2 and Ki-67 were analyzed in HeLa and human fibroblast cells after synchronization by double thymidine block.<h4>Results</h4>Although there was a significant correlation (R = 0.754, P < 0.001) between CPCC and Ki-67LI, only CPCC was correlated with DFS in overall population (HR, 2.029; 95% CI, 1.012-4.068; P = 0.046) and HER2-negative luminal subgroup (HR, 3.984; 95% CI, 1.350-11.762; P = 0.012) by multivariate analysis. In immunocytochemical staining, more than 50% of serum-starved or non-mitotic cell phase HeLa cells were positive for Ki-67, in comparison to the low CKAP2-positivity, which might explain the prognostic difference between CPCC and Ki-67LI.<h4>Conclusions</h4>The current study showed that CPCC but not Ki-67LI is an independent prognostic indicator in early breast cancer, more specifically in HER2-negative luminal breast cancer. The difference between two markers may be related to the lower background expression of CKAP2 in cancer cells.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182107&type=printable |
| spellingShingle | Sung Hoon Sim Chang-Dae Bae Youngmi Kwon Hai-Li Hwang Shiv Poojan Hye-In Hong Kyungtae Kim Seo-Hee Kang Han-Seong Kim Tae-Hyun Um In Hae Park Keun Seok Lee So-Youn Jung Seeyoun Lee Han-Sung Kang Eun Sook Lee Mi-Kyung Kim Kyeong-Man Hong Jungsil Ro CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. PLoS ONE |
| title | CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. |
| title_full | CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. |
| title_fullStr | CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. |
| title_full_unstemmed | CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. |
| title_short | CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast cancer. |
| title_sort | ckap2 cytoskeleton associated protein2 is a new prognostic marker in her2 negative luminal type breast cancer |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182107&type=printable |
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