Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage

Species of epiphytic microbiota are closely associated with the fermentation performance of natural forage silage. This study aimed to evaluate the dynamic microbial communities, fermentation parameters, and aerobic stability of Napier grass silage from the same variety and growth period but harvest...

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Main Authors: Hao Ding, Qi Yan, Nanji Zhang, Qichao Gu, Qingfeng Tang, Bo Lin, Caixia Zou
Format: Article
Language:English
Published: Cambridge University Press 2024-01-01
Series:Animal Nutriomics
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Online Access:https://www.cambridge.org/core/product/identifier/S2977377624000127/type/journal_article
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author Hao Ding
Qi Yan
Nanji Zhang
Qichao Gu
Qingfeng Tang
Bo Lin
Caixia Zou
author_facet Hao Ding
Qi Yan
Nanji Zhang
Qichao Gu
Qingfeng Tang
Bo Lin
Caixia Zou
author_sort Hao Ding
collection DOAJ
description Species of epiphytic microbiota are closely associated with the fermentation performance of natural forage silage. This study aimed to evaluate the dynamic microbial communities, fermentation parameters, and aerobic stability of Napier grass silage from the same variety and growth period but harvested from three different regions (NGP1, NGP2, and NGP3). After 60 days of ensiling, triplicate silos were opened for sampling and testing aerobic stability. The epiphytic microbiota with higher relative abundances in fresh Napier grass (NGP1, NGP2, and NGP3) were Weissella, Enterobacter, and Lactococcus, respectively. After 60 days of ensiling, NGP3 exhibited higher fermentation quality, indicated by higher lactic acid (LA) concentration and lower pH than that of NGP1 and NGP2. The NH3–N content of all treatments was lower than 100 g/kg total nitrogen. Compared with NGP1 and NGP2 silage, NGP3 silage exhibited a sharp rise in pH and LA consumption during air exposure. After 7 days of air exposure, NGP3 had higher ethanol concentrations and pH. Ruminiclostridium_5, Pediococcus, and Lactobacillus predominated in NGP1, NGP2, and NGP3 silages, respectively, whereas Candida and Monascus predominated in air-exposed NGP3 silage. The bacterial co-occurrence networks from fresh samples to ensiling and air exposure became more complex; however, NGP3 had a higher negative correlation with co-occurrence after air exposure. Different regions had significant effects on the fermentation patterns, bacterial communities, and aerobic stability of Napier grass silage. This was mainly due to variable epiphytic microbiota. Higher fermentation quality of Napier grass silage may also result in accelerated spoilage due to air exposure. Candida and Monascus were primarily responsible for the lower dry matter recovery and higher ethanol contents and air exposure spoilage of Napier grass silage.
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issn 2977-3776
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publishDate 2024-01-01
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series Animal Nutriomics
spelling doaj-art-4e64794a14b74cfcb211d708a6e3c6482025-08-20T03:41:15ZengCambridge University PressAnimal Nutriomics2977-37762977-37842024-01-01110.1017/anr.2024.12Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silageHao Ding0Qi Yan1Nanji Zhang2Qichao Gu3Qingfeng Tang4Bo Lin5Caixia Zou6https://orcid.org/0000-0003-1836-258XCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, China Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, ChinaSpecies of epiphytic microbiota are closely associated with the fermentation performance of natural forage silage. This study aimed to evaluate the dynamic microbial communities, fermentation parameters, and aerobic stability of Napier grass silage from the same variety and growth period but harvested from three different regions (NGP1, NGP2, and NGP3). After 60 days of ensiling, triplicate silos were opened for sampling and testing aerobic stability. The epiphytic microbiota with higher relative abundances in fresh Napier grass (NGP1, NGP2, and NGP3) were Weissella, Enterobacter, and Lactococcus, respectively. After 60 days of ensiling, NGP3 exhibited higher fermentation quality, indicated by higher lactic acid (LA) concentration and lower pH than that of NGP1 and NGP2. The NH3–N content of all treatments was lower than 100 g/kg total nitrogen. Compared with NGP1 and NGP2 silage, NGP3 silage exhibited a sharp rise in pH and LA consumption during air exposure. After 7 days of air exposure, NGP3 had higher ethanol concentrations and pH. Ruminiclostridium_5, Pediococcus, and Lactobacillus predominated in NGP1, NGP2, and NGP3 silages, respectively, whereas Candida and Monascus predominated in air-exposed NGP3 silage. The bacterial co-occurrence networks from fresh samples to ensiling and air exposure became more complex; however, NGP3 had a higher negative correlation with co-occurrence after air exposure. Different regions had significant effects on the fermentation patterns, bacterial communities, and aerobic stability of Napier grass silage. This was mainly due to variable epiphytic microbiota. Higher fermentation quality of Napier grass silage may also result in accelerated spoilage due to air exposure. Candida and Monascus were primarily responsible for the lower dry matter recovery and higher ethanol contents and air exposure spoilage of Napier grass silage.https://www.cambridge.org/core/product/identifier/S2977377624000127/type/journal_articleNapier grassfermentation qualitysilagemicrobial community
spellingShingle Hao Ding
Qi Yan
Nanji Zhang
Qichao Gu
Qingfeng Tang
Bo Lin
Caixia Zou
Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
Animal Nutriomics
Napier grass
fermentation quality
silage
microbial community
title Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
title_full Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
title_fullStr Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
title_full_unstemmed Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
title_short Regional variations in epiphytic microbiota influence fermentation quality, microbial communities, and aerobic stability of Napier grass silage
title_sort regional variations in epiphytic microbiota influence fermentation quality microbial communities and aerobic stability of napier grass silage
topic Napier grass
fermentation quality
silage
microbial community
url https://www.cambridge.org/core/product/identifier/S2977377624000127/type/journal_article
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