NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation
Abstract The accurate assessment of platelet activity is crucial in clinical practice and scientific research owing to the pivotal role of platelets in the progression of cardiovascular conditions, such as arterial thrombotic diseases. However, conventional platelet activity assessment methods are c...
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BMC
2025-03-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03212-5 |
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| author | Zheyi Jiang Meng Wei Jiawei Zhu Chenguang Wang Tiantian Zhang Wenjie Zhu Rong Zhang Kandi Zhang Peng Zhang Yihua Lu Alex Chia Yu Chang Yifan Liu Junfeng Zhang |
| author_facet | Zheyi Jiang Meng Wei Jiawei Zhu Chenguang Wang Tiantian Zhang Wenjie Zhu Rong Zhang Kandi Zhang Peng Zhang Yihua Lu Alex Chia Yu Chang Yifan Liu Junfeng Zhang |
| author_sort | Zheyi Jiang |
| collection | DOAJ |
| description | Abstract The accurate assessment of platelet activity is crucial in clinical practice and scientific research owing to the pivotal role of platelets in the progression of cardiovascular conditions, such as arterial thrombotic diseases. However, conventional platelet activity assessment methods are currently limited by their requirement of substantial blood samples and inadequate high-throughput capabilities, and therapeutic resistance induced by antiplatelet agents impedes treatment efficacy. In this study, we developed a microdroplet-based platelet function detection method, referred to as NebulaPlate, to achieve miniaturized and robust platelet activity assessment, thereby overcoming current challenges. NebulaPlate supports the merging of platelet samples with drugs confined in picoliter microdroplets and leverages an imaging-based analysis to automatically identify platelets, evaluate their aggregation, and determine P-selectin expression within the anchored microdroplets. We experimentally confirmed the feasibility of aggregation assays on NebulaPlate using various representative antiplatelet drugs. Requiring only 0.3 mL whole blood/chip, which corresponds to approximately 100 platelets/reaction, NebulaPlate reduced the consumption of platelet samples in a single assay. This represents a reduction of 10 times compared to that of conventional techniques. Moreover, our experimental results confirmed the validity and reproducibility of platelet function assays performed using NebulaPlate. Our research highlights important developments in the field of platelet activity assessment and provides fresh prospects for future antiplatelet therapies and personalized medicine. Moreover, it introduces new possibilities for research and clinical practice related to arterial thrombotic diseases. Graphical abstract |
| format | Article |
| id | doaj-art-4e6460e0b36340e893b5032c05bcdc62 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-4e6460e0b36340e893b5032c05bcdc622025-08-20T02:47:07ZengBMCJournal of Nanobiotechnology1477-31552025-03-0123111410.1186/s12951-025-03212-5NebulaPlate: a droplet microfluidic platform to analyze platelet aggregationZheyi Jiang0Meng Wei1Jiawei Zhu2Chenguang Wang3Tiantian Zhang4Wenjie Zhu5Rong Zhang6Kandi Zhang7Peng Zhang8Yihua Lu9Alex Chia Yu Chang10Yifan Liu11Junfeng Zhang12Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineSchool of Physical Science and Technology, ShanghaiTech UniversityShanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineSchool of Physical Science and Technology, ShanghaiTech UniversitySchool of Physical Science and Technology, ShanghaiTech UniversityDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineSchool of Physical Science and Technology, ShanghaiTech UniversityDepartment of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract The accurate assessment of platelet activity is crucial in clinical practice and scientific research owing to the pivotal role of platelets in the progression of cardiovascular conditions, such as arterial thrombotic diseases. However, conventional platelet activity assessment methods are currently limited by their requirement of substantial blood samples and inadequate high-throughput capabilities, and therapeutic resistance induced by antiplatelet agents impedes treatment efficacy. In this study, we developed a microdroplet-based platelet function detection method, referred to as NebulaPlate, to achieve miniaturized and robust platelet activity assessment, thereby overcoming current challenges. NebulaPlate supports the merging of platelet samples with drugs confined in picoliter microdroplets and leverages an imaging-based analysis to automatically identify platelets, evaluate their aggregation, and determine P-selectin expression within the anchored microdroplets. We experimentally confirmed the feasibility of aggregation assays on NebulaPlate using various representative antiplatelet drugs. Requiring only 0.3 mL whole blood/chip, which corresponds to approximately 100 platelets/reaction, NebulaPlate reduced the consumption of platelet samples in a single assay. This represents a reduction of 10 times compared to that of conventional techniques. Moreover, our experimental results confirmed the validity and reproducibility of platelet function assays performed using NebulaPlate. Our research highlights important developments in the field of platelet activity assessment and provides fresh prospects for future antiplatelet therapies and personalized medicine. Moreover, it introduces new possibilities for research and clinical practice related to arterial thrombotic diseases. Graphical abstracthttps://doi.org/10.1186/s12951-025-03212-5Platelet functionPlatelet aggregationMicrodropletP-selectinMicrofluidic chip |
| spellingShingle | Zheyi Jiang Meng Wei Jiawei Zhu Chenguang Wang Tiantian Zhang Wenjie Zhu Rong Zhang Kandi Zhang Peng Zhang Yihua Lu Alex Chia Yu Chang Yifan Liu Junfeng Zhang NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation Journal of Nanobiotechnology Platelet function Platelet aggregation Microdroplet P-selectin Microfluidic chip |
| title | NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation |
| title_full | NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation |
| title_fullStr | NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation |
| title_full_unstemmed | NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation |
| title_short | NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation |
| title_sort | nebulaplate a droplet microfluidic platform to analyze platelet aggregation |
| topic | Platelet function Platelet aggregation Microdroplet P-selectin Microfluidic chip |
| url | https://doi.org/10.1186/s12951-025-03212-5 |
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