Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability
lncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (p...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.731712/full |
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| author | Wei Lv Wei Lv Shiyu Zhao Shiyu Zhao Yunqing Hou Yunqing Hou Qian Tong Qian Tong Yaxin Peng Yaxin Peng Jianan Li Jianan Li Zaiyan Xu Zaiyan Xu Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo |
| author_facet | Wei Lv Wei Lv Shiyu Zhao Shiyu Zhao Yunqing Hou Yunqing Hou Qian Tong Qian Tong Yaxin Peng Yaxin Peng Jianan Li Jianan Li Zaiyan Xu Zaiyan Xu Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo |
| author_sort | Wei Lv |
| collection | DOAJ |
| description | lncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (plncMGPF) gene were identified among commercial pig breeds and Chinese local pig breeds. These SNPs are highly linked and constructed into multiple haplotypes, and haplotype ATTCATGTTC (H1) mainly exists in commercial pig breeds while haplotype GCCTGCACCT (H3) is more frequent in Chinese local pig breeds. Association analysis indicated that all SNPs are significantly associated with the backfat thickness and loin muscle area (P < 0.05), respectively, and homologous H1 individuals have higher loin muscle area and lower backfat thickness than H3 pigs. Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. Further experiments showed that two SNPs (rs81403974 and rs325492834) function together to confer plncMGPF stability and function. Our observation suggested that the SNPs in lncMGPF can change the RNA stabilities and lncMGPF function, thereby affecting the porcine meat production traits. |
| format | Article |
| id | doaj-art-4e5fa8145d13443fb94f485744b8d3e8 |
| institution | DOAJ |
| issn | 2296-634X |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-4e5fa8145d13443fb94f485744b8d3e82025-08-20T02:49:49ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.731712731712Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA StabilityWei Lv0Wei Lv1Shiyu Zhao2Shiyu Zhao3Yunqing Hou4Yunqing Hou5Qian Tong6Qian Tong7Yaxin Peng8Yaxin Peng9Jianan Li10Jianan Li11Zaiyan Xu12Zaiyan Xu13Bo Zuo14Bo Zuo15Bo Zuo16Bo Zuo17Bo Zuo18Bo Zuo19Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaShenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinalncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (plncMGPF) gene were identified among commercial pig breeds and Chinese local pig breeds. These SNPs are highly linked and constructed into multiple haplotypes, and haplotype ATTCATGTTC (H1) mainly exists in commercial pig breeds while haplotype GCCTGCACCT (H3) is more frequent in Chinese local pig breeds. Association analysis indicated that all SNPs are significantly associated with the backfat thickness and loin muscle area (P < 0.05), respectively, and homologous H1 individuals have higher loin muscle area and lower backfat thickness than H3 pigs. Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. Further experiments showed that two SNPs (rs81403974 and rs325492834) function together to confer plncMGPF stability and function. Our observation suggested that the SNPs in lncMGPF can change the RNA stabilities and lncMGPF function, thereby affecting the porcine meat production traits.https://www.frontiersin.org/articles/10.3389/fcell.2021.731712/fulllncMGPFSNPsmeat production traitsRNA stabilitypig |
| spellingShingle | Wei Lv Wei Lv Shiyu Zhao Shiyu Zhao Yunqing Hou Yunqing Hou Qian Tong Qian Tong Yaxin Peng Yaxin Peng Jianan Li Jianan Li Zaiyan Xu Zaiyan Xu Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo Bo Zuo Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability Frontiers in Cell and Developmental Biology lncMGPF SNPs meat production traits RNA stability pig |
| title | Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability |
| title_full | Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability |
| title_fullStr | Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability |
| title_full_unstemmed | Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability |
| title_short | Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability |
| title_sort | single nucleotide polymorphisms of porcine lncmgpf regulate meat production traits by affecting rna stability |
| topic | lncMGPF SNPs meat production traits RNA stability pig |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.731712/full |
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