Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice
Introduction: Thymus vulgaris is a plant used in Cameroon to alleviate and treat hangover symptoms. This study was conducted to assess the anxiolytic, memory-improving, hepatoprotective, and antioxidant effects of T. vulgaris on alcohol withdrawal syndrome. Methods: In this preclinical study 42 mice...
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Shahrekord University of Medical Sciences
2024-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | https://herbmedpharmacol.com/PDF/jhp-13-572.pdf |
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| author | Nadège Emégam Kouémou Franklin Mbeboh Savo Simon Pale Aimé Paul Noubissi Sylviane Mireille Nguepi Dongmo Germain Sotoing Taiwe Elisabeth Ngo Bum |
| author_facet | Nadège Emégam Kouémou Franklin Mbeboh Savo Simon Pale Aimé Paul Noubissi Sylviane Mireille Nguepi Dongmo Germain Sotoing Taiwe Elisabeth Ngo Bum |
| author_sort | Nadège Emégam Kouémou |
| collection | DOAJ |
| description | Introduction: Thymus vulgaris is a plant used in Cameroon to alleviate and treat hangover symptoms. This study was conducted to assess the anxiolytic, memory-improving, hepatoprotective, and antioxidant effects of T. vulgaris on alcohol withdrawal syndrome. Methods: In this preclinical study 42 mice were grouped into seven equal sets of normal control, negative control, positive control (piracetam; 200 mg/kg), and test (T. vulgaris; 25, 50, 100, or 200 mg/kg) groups. All mice (except those of the normal control) were administered ethanol by gavage once daily for 28 days. After alcohol withdrawal, behavioral assessments (elevated plus maze, Y-maze, and open field) were performed from day 29 to day 31, one hour following appropriate treatments. Mice were euthanized on day 32 and the brains and the livers were used for histopathological assessment and oxidative stress parameters evaluation. Results: In the elevated plus maze test, T. vulgaris at 100 and 200 mg/kg significantly (P < 0.001) increased the number of entries and the time spent in the open arms. The plant extract (200 mg/kg) significantly (P < 0.001) increased the spontaneous alternation percentage in the Y maze. T. vulgaris administration decreased malondialdehyde (MDA) and nitric oxide (NO) in the brain and liver and increased glutathione (GSH) and catalase (CAT). The plant extract reduced the neuronal degeneration and hepatocyte damage induced by chronic alcohol administration. Conclusion: T. vulgaris had anxiolytic, memory-enhancing, and hepatoprotective properties against alcohol toxicity. These results may justify the use of thyme in traditional pharmacopeia in the management of alcohol use disorders (AUDs). |
| format | Article |
| id | doaj-art-4e5c7efa9f954761b3024c8daa82cbf7 |
| institution | DOAJ |
| issn | 2345-5004 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj-art-4e5c7efa9f954761b3024c8daa82cbf72025-08-20T02:51:34ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042024-10-0113457258610.34172/jhp.2024.51455jhp-51455Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in miceNadège Emégam Kouémou0Franklin Mbeboh Savo1Simon Pale2Aimé Paul Noubissi3Sylviane Mireille Nguepi Dongmo4Germain Sotoing Taiwe5Elisabeth Ngo Bum6Department of Animal Biology and Conservation, Faculty of Science, University of Buea, Cameroon P.O. Box 63, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Cameroon P.O. Box 63, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Cameroon P.O. Box 63, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Cameroon P.O. Box 63, CameroonDepartment of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, P.O. Box 63, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Cameroon P.O. Box 63, CameroonDepartment of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 52, Maroua, CameroonIntroduction: Thymus vulgaris is a plant used in Cameroon to alleviate and treat hangover symptoms. This study was conducted to assess the anxiolytic, memory-improving, hepatoprotective, and antioxidant effects of T. vulgaris on alcohol withdrawal syndrome. Methods: In this preclinical study 42 mice were grouped into seven equal sets of normal control, negative control, positive control (piracetam; 200 mg/kg), and test (T. vulgaris; 25, 50, 100, or 200 mg/kg) groups. All mice (except those of the normal control) were administered ethanol by gavage once daily for 28 days. After alcohol withdrawal, behavioral assessments (elevated plus maze, Y-maze, and open field) were performed from day 29 to day 31, one hour following appropriate treatments. Mice were euthanized on day 32 and the brains and the livers were used for histopathological assessment and oxidative stress parameters evaluation. Results: In the elevated plus maze test, T. vulgaris at 100 and 200 mg/kg significantly (P < 0.001) increased the number of entries and the time spent in the open arms. The plant extract (200 mg/kg) significantly (P < 0.001) increased the spontaneous alternation percentage in the Y maze. T. vulgaris administration decreased malondialdehyde (MDA) and nitric oxide (NO) in the brain and liver and increased glutathione (GSH) and catalase (CAT). The plant extract reduced the neuronal degeneration and hepatocyte damage induced by chronic alcohol administration. Conclusion: T. vulgaris had anxiolytic, memory-enhancing, and hepatoprotective properties against alcohol toxicity. These results may justify the use of thyme in traditional pharmacopeia in the management of alcohol use disorders (AUDs).https://herbmedpharmacol.com/PDF/jhp-13-572.pdfalcoholanxietyantioxidantmemoryliverthymus vulgaris |
| spellingShingle | Nadège Emégam Kouémou Franklin Mbeboh Savo Simon Pale Aimé Paul Noubissi Sylviane Mireille Nguepi Dongmo Germain Sotoing Taiwe Elisabeth Ngo Bum Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice Journal of HerbMed Pharmacology alcohol anxiety antioxidant memory liver thymus vulgaris |
| title | Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice |
| title_full | Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice |
| title_fullStr | Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice |
| title_full_unstemmed | Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice |
| title_short | Anxiolytic, memory improvement, hepatoprotective, and antioxidant effects of Thymus vulgaris on alcohol withdrawal syndrome in mice |
| title_sort | anxiolytic memory improvement hepatoprotective and antioxidant effects of thymus vulgaris on alcohol withdrawal syndrome in mice |
| topic | alcohol anxiety antioxidant memory liver thymus vulgaris |
| url | https://herbmedpharmacol.com/PDF/jhp-13-572.pdf |
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