Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis
Background and Aims: The Mediterranean diet pattern (MDP) is associated with health-associated gut microbes and metabolites. However, the impact of the MDP on the fecal metabolome in ulcerative colitis (UC) remains unclear. We characterized the fecal metabolome of patients with UC with high adherenc...
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Elsevier
2025-01-01
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| Series: | Gastro Hep Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772572324002024 |
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| author | Natasha Haskey Jiayu Ye Jessica Josephson Maitreyi Raman Sanjoy Ghosh Deanna L. Gibson |
| author_facet | Natasha Haskey Jiayu Ye Jessica Josephson Maitreyi Raman Sanjoy Ghosh Deanna L. Gibson |
| author_sort | Natasha Haskey |
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| description | Background and Aims: The Mediterranean diet pattern (MDP) is associated with health-associated gut microbes and metabolites. However, the impact of the MDP on the fecal metabolome in ulcerative colitis (UC) remains unclear. We characterized the fecal metabolome of patients with UC with high adherence to the MDP compared to the Canadian habitual diet (CHD). Furthermore, we explored potential differences in the fecal metabolome between dietary responders and nonresponders to the MDP. Methods: Utilizing untargeted metabolomics on a subset of fecal samples obtained from a randomized controlled trial, adult patients with quiescent UC underwent a 12-week intervention following either the MDP (n = 8) or CHD (n = 8). Liquid chromatography-tandem mass spectrometry was employed to profile endogenous fecal metabolites, while 16S amplicon sequencing was utilized to profile the fecal microbiota. Results: A total of 701 human metabolites were detected, with 35 exhibiting significant differential expression between the MDP and CHD groups. Noteworthy, folate biosynthesis, sphingolipid biosynthesis, and steroid biosynthesis were identified as major pathways affected. Moreover, microbial analysis showed that individuals with increased levels of the class Bacteroidia (Bacteroides vulgatus [B. vulgatus], B. uniformis, and B. acidifaciens) in their stool at baseline were more likely to respond to the MDP. Conclusion: High adherence to an MDP is associated with beneficial metabolite changes associated with reducing inflammation in UC. In addition, fiber-degrading microbes abundant before dietary intervention played a role in the responsiveness to the MDP. This work lays the groundwork for developing a metabolic signature associated with the MDP to develop personalized nutrition strategies for UC prevention and treatment. ClinicalTrials.gov Number: NCT03053713. |
| format | Article |
| id | doaj-art-4e58c8346a914ee88e6b895184dffc2b |
| institution | OA Journals |
| issn | 2772-5723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Gastro Hep Advances |
| spelling | doaj-art-4e58c8346a914ee88e6b895184dffc2b2025-08-20T01:55:52ZengElsevierGastro Hep Advances2772-57232025-01-014410060610.1016/j.gastha.2024.100606Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative ColitisNatasha Haskey0Jiayu Ye1Jessica Josephson2Maitreyi Raman3Sanjoy Ghosh4Deanna L. Gibson5Department of Biology, University of British Columbia - Okanagan Campus, Kelowna, British Columbia, CanadaDivision of Gastroenterology and Hepatology, School of Medicine, Stanford University, Palo Alto, CaliforniaDepartment of Biology, University of British Columbia - Okanagan Campus, Kelowna, British Columbia, CanadaDepartment of Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus, Kelowna, British Columbia, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus, Kelowna, British Columbia, Canada; Southern Medical Program, Faculty of Medicine, University of British Columbia–Okanagan, Kelowna, British Columbia, Canada; Correspondence: Address correspondence to: Deanna L. Gibson, Department of Biology, University of British Columbia-Okanagan, 3137 University Way, Kelowna, British Columbia V1V 1V7, Canada.Background and Aims: The Mediterranean diet pattern (MDP) is associated with health-associated gut microbes and metabolites. However, the impact of the MDP on the fecal metabolome in ulcerative colitis (UC) remains unclear. We characterized the fecal metabolome of patients with UC with high adherence to the MDP compared to the Canadian habitual diet (CHD). Furthermore, we explored potential differences in the fecal metabolome between dietary responders and nonresponders to the MDP. Methods: Utilizing untargeted metabolomics on a subset of fecal samples obtained from a randomized controlled trial, adult patients with quiescent UC underwent a 12-week intervention following either the MDP (n = 8) or CHD (n = 8). Liquid chromatography-tandem mass spectrometry was employed to profile endogenous fecal metabolites, while 16S amplicon sequencing was utilized to profile the fecal microbiota. Results: A total of 701 human metabolites were detected, with 35 exhibiting significant differential expression between the MDP and CHD groups. Noteworthy, folate biosynthesis, sphingolipid biosynthesis, and steroid biosynthesis were identified as major pathways affected. Moreover, microbial analysis showed that individuals with increased levels of the class Bacteroidia (Bacteroides vulgatus [B. vulgatus], B. uniformis, and B. acidifaciens) in their stool at baseline were more likely to respond to the MDP. Conclusion: High adherence to an MDP is associated with beneficial metabolite changes associated with reducing inflammation in UC. In addition, fiber-degrading microbes abundant before dietary intervention played a role in the responsiveness to the MDP. This work lays the groundwork for developing a metabolic signature associated with the MDP to develop personalized nutrition strategies for UC prevention and treatment. ClinicalTrials.gov Number: NCT03053713.http://www.sciencedirect.com/science/article/pii/S2772572324002024Mediterranean DietInflammatory Bowel DiseaseMetabolomeUlcerative Colitis |
| spellingShingle | Natasha Haskey Jiayu Ye Jessica Josephson Maitreyi Raman Sanjoy Ghosh Deanna L. Gibson Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis Gastro Hep Advances Mediterranean Diet Inflammatory Bowel Disease Metabolome Ulcerative Colitis |
| title | Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis |
| title_full | Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis |
| title_fullStr | Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis |
| title_full_unstemmed | Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis |
| title_short | Metabolomic Signatures Highlight Fiber-Degrading Bacteroides Species in Mediterranean Diet Response Among Ulcerative Colitis |
| title_sort | metabolomic signatures highlight fiber degrading bacteroides species in mediterranean diet response among ulcerative colitis |
| topic | Mediterranean Diet Inflammatory Bowel Disease Metabolome Ulcerative Colitis |
| url | http://www.sciencedirect.com/science/article/pii/S2772572324002024 |
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