Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice
Abstract The present research was set out to delineate the protective and therapeutic potency of hesperidin (Hesp) versus cisplatin (Cis) against the deleterious consequences of Ehrlich ascites carcinoma (EAC) on the liver and the prospective mitigative effect of Hesp against Cis-mediated hepatotoxi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-06-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-02442-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849420501276950528 |
|---|---|
| author | Nahed Saleh Tamer Allam Reda M. S. Korany Abdelfattah M. Abdelfattah Ahmed M. Omran Mabrouk Attia Abd Eldaim Nermeen Borai El-Borai |
| author_facet | Nahed Saleh Tamer Allam Reda M. S. Korany Abdelfattah M. Abdelfattah Ahmed M. Omran Mabrouk Attia Abd Eldaim Nermeen Borai El-Borai |
| author_sort | Nahed Saleh |
| collection | DOAJ |
| description | Abstract The present research was set out to delineate the protective and therapeutic potency of hesperidin (Hesp) versus cisplatin (Cis) against the deleterious consequences of Ehrlich ascites carcinoma (EAC) on the liver and the prospective mitigative effect of Hesp against Cis-mediated hepatotoxic side-effects. A total of 70 female mice were randomly assigned into control, Hesp, EAC, Hesp-protected, Hesp-treated, Cis-treated, and Cis + Hesp-treated groups. Mice inoculated with EAC cells exhibited significant reductions in the serum total protein and albumin levels, along with significant elevations of the serum aminotransferases, lactate dehydrogenase, amylase, and lipase activities, and alpha-fetoprotein level. A significant increment in malondialdehyde level concomitantly with significant declines in reduced glutathione concentration and catalase activity were also observed in the liver of EAC-bearing mice. Additionally, marked hepatic pathological changes as well as a strong Ki-67 expression and a weak caspase-3 expression in the neoplastic cells infiltrating hepatocytes were observed. In contrast, the administration of Hesp and/or Cis to the EAC-bearing mice reversed, to varying degrees, the cytotoxic effects of EAC. Besides, Hesp minimized the harmful hepatic chemotherapeutic side-effects of Cis. Overall, Hesp could be a promising phytochemical against EAC-induced cytotoxicity with its potential to improve the antitumor efficacy of chemotherapeutic drugs and minimize their hepatic adverse side-effects. |
| format | Article |
| id | doaj-art-4e5057e69a1149d68e93f9fa9543ca66 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4e5057e69a1149d68e93f9fa9543ca662025-08-20T03:31:44ZengNature PortfolioScientific Reports2045-23222025-06-0115111310.1038/s41598-025-02442-9Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in miceNahed Saleh0Tamer Allam1Reda M. S. Korany2Abdelfattah M. Abdelfattah3Ahmed M. Omran4Mabrouk Attia Abd Eldaim5Nermeen Borai El-Borai6Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Pathology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Menoufia UniversityDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat CityAbstract The present research was set out to delineate the protective and therapeutic potency of hesperidin (Hesp) versus cisplatin (Cis) against the deleterious consequences of Ehrlich ascites carcinoma (EAC) on the liver and the prospective mitigative effect of Hesp against Cis-mediated hepatotoxic side-effects. A total of 70 female mice were randomly assigned into control, Hesp, EAC, Hesp-protected, Hesp-treated, Cis-treated, and Cis + Hesp-treated groups. Mice inoculated with EAC cells exhibited significant reductions in the serum total protein and albumin levels, along with significant elevations of the serum aminotransferases, lactate dehydrogenase, amylase, and lipase activities, and alpha-fetoprotein level. A significant increment in malondialdehyde level concomitantly with significant declines in reduced glutathione concentration and catalase activity were also observed in the liver of EAC-bearing mice. Additionally, marked hepatic pathological changes as well as a strong Ki-67 expression and a weak caspase-3 expression in the neoplastic cells infiltrating hepatocytes were observed. In contrast, the administration of Hesp and/or Cis to the EAC-bearing mice reversed, to varying degrees, the cytotoxic effects of EAC. Besides, Hesp minimized the harmful hepatic chemotherapeutic side-effects of Cis. Overall, Hesp could be a promising phytochemical against EAC-induced cytotoxicity with its potential to improve the antitumor efficacy of chemotherapeutic drugs and minimize their hepatic adverse side-effects.https://doi.org/10.1038/s41598-025-02442-9CisplatinEhrlich ascites carcinomaHesperidinAlpha fetoproteinCaspase-3Ki-67 |
| spellingShingle | Nahed Saleh Tamer Allam Reda M. S. Korany Abdelfattah M. Abdelfattah Ahmed M. Omran Mabrouk Attia Abd Eldaim Nermeen Borai El-Borai Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice Scientific Reports Cisplatin Ehrlich ascites carcinoma Hesperidin Alpha fetoprotein Caspase-3 Ki-67 |
| title | Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice |
| title_full | Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice |
| title_fullStr | Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice |
| title_full_unstemmed | Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice |
| title_short | Hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of Ehrlich ascites carcinoma in mice |
| title_sort | hesperidin exacerbates the therapeutic potency of cisplatin against hepatocytotoxicity of ehrlich ascites carcinoma in mice |
| topic | Cisplatin Ehrlich ascites carcinoma Hesperidin Alpha fetoprotein Caspase-3 Ki-67 |
| url | https://doi.org/10.1038/s41598-025-02442-9 |
| work_keys_str_mv | AT nahedsaleh hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT tamerallam hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT redamskorany hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT abdelfattahmabdelfattah hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT ahmedmomran hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT mabroukattiaabdeldaim hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice AT nermeenboraielborai hesperidinexacerbatesthetherapeuticpotencyofcisplatinagainsthepatocytotoxicityofehrlichascitescarcinomainmice |