Sensitive Detection of Aflatoxin B1 in Foods Using Aptasensing Based on FGO-Mediated CdTe QDs
Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) exhibits high toxicity and has the potential to induce cancer, deformities, and mutations. It is therefore highly desirable that sensitive and straightforward methods for detecting AFB<sub>1</sub> be developed. In this stu...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/13/4/141 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) exhibits high toxicity and has the potential to induce cancer, deformities, and mutations. It is therefore highly desirable that sensitive and straightforward methods for detecting AFB<sub>1</sub> be developed. In this study, due to the high specific adsorption capacity of AFB<sub>1</sub> aptamers, we applied a sensing strategy based on quantum dots (QDs) and carboxyl-functionalized graphene oxide (FGO) to construct a simple fluorescence quenching platform. FGO and CdTe QDs modified with AFB<sub>1</sub> aptamers cause a FRET effect that produces CdTe QDs with yellow-green fluorescence quenching. When AFB<sub>1</sub> is present, aptamers form complexes with it and CdTe QDs leave the quenching platform, resulting in fluorescence recovery. In this study, we used a fluorescence aptasensor with a wide detection range of 0.05 to 150 ng/mL and a low limit of detection (LOD) of 8.2 pg/mL. The average recoveries of AFB<sub>1</sub> in peanut and pure milk samples ranged from 94.5% to 107.0%. The aptasensor also exhibited the advantages of simple operation, low cost, and good stability. The sensing strategy reported here can thus serve as a potential candidate for the rapid detection of AFB<sub>1.</sub> |
|---|---|
| ISSN: | 2227-9040 |