Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide
Using Polygonatum sibiricum as raw material, P. sibiricum polysaccharide was extracted by enzyme assisted method and the extraction process was optimized by single factor and orthogonal tests. The moisture absorption and moisture retention tests were carried out with P. sibiricum polysaccharide frag...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-06-01
|
| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-6-245.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850205470535450624 |
|---|---|
| author | GULIZHADANMU Dawuti, LIU Xiaofeng, YANG Jiajun, MA Wenjin, ZHANG Kui, LI Jun, WEI Kongbin, LV Zhigang |
| author_facet | GULIZHADANMU Dawuti, LIU Xiaofeng, YANG Jiajun, MA Wenjin, ZHANG Kui, LI Jun, WEI Kongbin, LV Zhigang |
| author_sort | GULIZHADANMU Dawuti, LIU Xiaofeng, YANG Jiajun, MA Wenjin, ZHANG Kui, LI Jun, WEI Kongbin, LV Zhigang |
| collection | DOAJ |
| description | Using Polygonatum sibiricum as raw material, P. sibiricum polysaccharide was extracted by enzyme assisted method and the extraction process was optimized by single factor and orthogonal tests. The moisture absorption and moisture retention tests were carried out with P. sibiricum polysaccharide fragments of different molecular weight obtained by dialysis, and the antioxidant capacity and structural characterization of the optimal moisturizing fragments were investigated. The results showed that the polysaccharide yield reached 24.18% under the conditions of compound enzyme addition 6%, enzymolysis time 90 min, enzymolysis temperature 50 ℃, solid and liquid ratio 1∶30 (g∶ml). When the mass concentration of the optimal moisturing fragment of P. sibiricum was more than 8 000 Da, its moisturing property was optimal. When the mass concentration of the optimal moisturing fragment of P. sibiricum polysaccharide was 6 mg/ml, the scavenging rates of DPPH, ABTS<sup>+</sup> and hydroxyl radicals were 50.54%, 48.65% and 26.41%, respectively, which showed certain antioxidant activities. At the same time, it was found that the surface of moisturing fragment was basically smooth, with irregular flakes and intermolecular functional groups such as -OH, C-H and C=O. |
| format | Article |
| id | doaj-art-4e3ebaeb1c5146e191ddd3985ea82f64 |
| institution | OA Journals |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-4e3ebaeb1c5146e191ddd3985ea82f642025-08-20T02:11:05ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-06-0143624525110.11882/j.issn.0254-5071.2024.06.038Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharideGULIZHADANMU Dawuti, LIU Xiaofeng, YANG Jiajun, MA Wenjin, ZHANG Kui, LI Jun, WEI Kongbin, LV Zhigang01. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; ;2. Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Longdong College, Qingyang 745000, China; ;3. Lanzhou Shibai Agricultural Biotechnology Co., Ltd., Lanzhou 730050, China; ;4. Agricultural Industrialization Office of Qilihe District, Lanzhou 730050, China; ;5. Agricultural Technology Extension Station of Qilihe District, Lanzhou 730050, ChinaUsing Polygonatum sibiricum as raw material, P. sibiricum polysaccharide was extracted by enzyme assisted method and the extraction process was optimized by single factor and orthogonal tests. The moisture absorption and moisture retention tests were carried out with P. sibiricum polysaccharide fragments of different molecular weight obtained by dialysis, and the antioxidant capacity and structural characterization of the optimal moisturizing fragments were investigated. The results showed that the polysaccharide yield reached 24.18% under the conditions of compound enzyme addition 6%, enzymolysis time 90 min, enzymolysis temperature 50 ℃, solid and liquid ratio 1∶30 (g∶ml). When the mass concentration of the optimal moisturing fragment of P. sibiricum was more than 8 000 Da, its moisturing property was optimal. When the mass concentration of the optimal moisturing fragment of P. sibiricum polysaccharide was 6 mg/ml, the scavenging rates of DPPH, ABTS<sup>+</sup> and hydroxyl radicals were 50.54%, 48.65% and 26.41%, respectively, which showed certain antioxidant activities. At the same time, it was found that the surface of moisturing fragment was basically smooth, with irregular flakes and intermolecular functional groups such as -OH, C-H and C=O.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-6-245.pdfpolygonatum sibiricum polysaccharide|process optimization|moisture absorption and retention|antioxidant activity|structural characterization |
| spellingShingle | GULIZHADANMU Dawuti, LIU Xiaofeng, YANG Jiajun, MA Wenjin, ZHANG Kui, LI Jun, WEI Kongbin, LV Zhigang Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide Zhongguo niangzao polygonatum sibiricum polysaccharide|process optimization|moisture absorption and retention|antioxidant activity|structural characterization |
| title | Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide |
| title_full | Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide |
| title_fullStr | Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide |
| title_full_unstemmed | Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide |
| title_short | Optimization of enzyme assisted extraction process and activity analysis of Polygonatum sibiricum polysaccharide |
| title_sort | optimization of enzyme assisted extraction process and activity analysis of polygonatum sibiricum polysaccharide |
| topic | polygonatum sibiricum polysaccharide|process optimization|moisture absorption and retention|antioxidant activity|structural characterization |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-6-245.pdf |
| work_keys_str_mv | AT gulizhadanmudawutiliuxiaofengyangjiajunmawenjinzhangkuilijunweikongbinlvzhigang optimizationofenzymeassistedextractionprocessandactivityanalysisofpolygonatumsibiricumpolysaccharide |