Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains
In the industrial production of <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i>, slow growth of the mother seed and insufficient hyphal vitality can significantly affect the cultivation process. To shorten the growth period on traditional PDA medium, two strains of <i...
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MDPI AG
2024-11-01
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| author | Zhanghu Chen Ruiheng Yang Yan Li Lihua Tang Huiyang Xiong Dapeng Bao Ting Guo |
| author_facet | Zhanghu Chen Ruiheng Yang Yan Li Lihua Tang Huiyang Xiong Dapeng Bao Ting Guo |
| author_sort | Zhanghu Chen |
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| description | In the industrial production of <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i>, slow growth of the mother seed and insufficient hyphal vitality can significantly affect the cultivation process. To shorten the growth period on traditional PDA medium, two strains of <i>L. edodes</i> and <i>P. eryngii</i> were cultured with different proportions of <i>P</i>. <i>tuber-regium</i> and <i>Wolfiporia hoelen</i> sclerotium powders added into the medium to investigate the effect on the mycelial growth. Compared to the PDA, the addition of sclerotia powder significantly enhanced the growth of mycelia, with an optimal addition ratio of 2%. Transcriptome sequencing was performed after culturing <i>L. edodes</i> and <i>P. eryngii</i> on PDA, PDA with 2% <i>P. tuber-regium</i> sclerotium powder, and PDA with 2% <i>W. hoelen</i> sclerotium powder. GO enrichment analysis of the differentially expressed genes (DEGs) of <i>L. edodes</i> and <i>P. eryngii</i> strains cultured in the sclerotia powder media showed significant changes in oxidoreductase and glucosidase activities. Changes were observed in KEGG annotation for carbohydrate metabolism, glycolysis, pyruvate metabolism, and other energy metabolic pathways. Moreover, carbohydrate-active enzyme (CAZyme) family genes were predominantly upregulated. The increase in the activity of CAZyme and oxidoreductases promotes the degradation of nutrients in the sclerotia into small-molecule substances, which explains why the sclerotia powder culture medium promotes mycelial growth. |
| format | Article |
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| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-4e34d4fb10e44396bdfcfb1d8c03af3a2025-08-20T02:56:59ZengMDPI AGJournal of Fungi2309-608X2024-11-01101280810.3390/jof10120808Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> StrainsZhanghu Chen0Ruiheng Yang1Yan Li2Lihua Tang3Huiyang Xiong4Dapeng Bao5Ting Guo6National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaIn the industrial production of <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i>, slow growth of the mother seed and insufficient hyphal vitality can significantly affect the cultivation process. To shorten the growth period on traditional PDA medium, two strains of <i>L. edodes</i> and <i>P. eryngii</i> were cultured with different proportions of <i>P</i>. <i>tuber-regium</i> and <i>Wolfiporia hoelen</i> sclerotium powders added into the medium to investigate the effect on the mycelial growth. Compared to the PDA, the addition of sclerotia powder significantly enhanced the growth of mycelia, with an optimal addition ratio of 2%. Transcriptome sequencing was performed after culturing <i>L. edodes</i> and <i>P. eryngii</i> on PDA, PDA with 2% <i>P. tuber-regium</i> sclerotium powder, and PDA with 2% <i>W. hoelen</i> sclerotium powder. GO enrichment analysis of the differentially expressed genes (DEGs) of <i>L. edodes</i> and <i>P. eryngii</i> strains cultured in the sclerotia powder media showed significant changes in oxidoreductase and glucosidase activities. Changes were observed in KEGG annotation for carbohydrate metabolism, glycolysis, pyruvate metabolism, and other energy metabolic pathways. Moreover, carbohydrate-active enzyme (CAZyme) family genes were predominantly upregulated. The increase in the activity of CAZyme and oxidoreductases promotes the degradation of nutrients in the sclerotia into small-molecule substances, which explains why the sclerotia powder culture medium promotes mycelial growth.https://www.mdpi.com/2309-608X/10/12/808<i>Lentinula edodes</i><i>Pleurotus eryngii</i>sclerotiumtranscriptomemedium compositionenergy metabolism |
| spellingShingle | Zhanghu Chen Ruiheng Yang Yan Li Lihua Tang Huiyang Xiong Dapeng Bao Ting Guo Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains Journal of Fungi <i>Lentinula edodes</i> <i>Pleurotus eryngii</i> sclerotium transcriptome medium composition energy metabolism |
| title | Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains |
| title_full | Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains |
| title_fullStr | Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains |
| title_full_unstemmed | Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains |
| title_short | Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder on <i>Lentinula edodes</i> and <i>Pleurotus eryngii</i> Strains |
| title_sort | transcriptome analysis of the growth promoting effect of large macrofungal sclerotium powder on i lentinula edodes i and i pleurotus eryngii i strains |
| topic | <i>Lentinula edodes</i> <i>Pleurotus eryngii</i> sclerotium transcriptome medium composition energy metabolism |
| url | https://www.mdpi.com/2309-608X/10/12/808 |
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