EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO

Shoot tips and nodes of two carnation cultivars "Jeanne Dionis Blano" a white flower cv. and "Marie Chabaud Jaune" a yellow flower cv. taken from the field were planted on MS medium supplemented with kinetin at (0.0 , 2.0 , 4.0, 6.0 , 8.0) ml/L. Shoots of "Jeanne Dionis...

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Main Author: Basher Z. A. Kassab Bashy
Format: Article
Language:English
Published: College of Agriculture 2010-04-01
Series:Mesopotamia Journal of Agriculture
Online Access:https://magrj.mosuljournals.com/article_33632_0e0c6eb0c33b9e2675a944113149539f.pdf
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author Basher Z. A. Kassab Bashy
author_facet Basher Z. A. Kassab Bashy
author_sort Basher Z. A. Kassab Bashy
collection DOAJ
description Shoot tips and nodes of two carnation cultivars "Jeanne Dionis Blano" a white flower cv. and "Marie Chabaud Jaune" a yellow flower cv. taken from the field were planted on MS medium supplemented with kinetin at (0.0 , 2.0 , 4.0, 6.0 , 8.0) ml/L. Shoots of "Jeanne Dionis Blano" produced in vitro were rooted on half strength MS medium supplemented with IAA . Data shows that shoot tip response were 100% for in vitro culture . There is no significant differences on shoot numbers between the two cultivars in multiplication stage, shoot tips planting on MS medium supplemented with 8.0mg/L Kin gave the highest number 14.95 shoot/explant in multiplication stage and gave 16.90 shoot/explant for white cv., no significant differences between cultivars from culture node of the two cultivars at the multiplication stage highest shoots number 6.07 shoot/explant were obtained from the node culture on MS medium at 2.0 mg/L Kin and the highest shoot number 12.70 shoot were obtained from shoot tip culture of yellow cv. on MS medium supplemented with 8.0 mg/L Kin, shoots which produced in vitro for white cv. gave the highest rooting 50% with the highest root number 1.75 root/shoot when culture on half strength MS medium supplemented with 1.5 mg /L IAA . All the plantlets which produced in vitro acclimatized in laboratory then transferred to the greenhouse to grow normally.
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spelling doaj-art-4e2ec4db3238428c82ea1f77d29e4ee92025-08-20T03:36:41ZengCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962010-04-0138021222310.33899/magrj.2010.3363233632EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITROBasher Z. A. Kassab BashyShoot tips and nodes of two carnation cultivars "Jeanne Dionis Blano" a white flower cv. and "Marie Chabaud Jaune" a yellow flower cv. taken from the field were planted on MS medium supplemented with kinetin at (0.0 , 2.0 , 4.0, 6.0 , 8.0) ml/L. Shoots of "Jeanne Dionis Blano" produced in vitro were rooted on half strength MS medium supplemented with IAA . Data shows that shoot tip response were 100% for in vitro culture . There is no significant differences on shoot numbers between the two cultivars in multiplication stage, shoot tips planting on MS medium supplemented with 8.0mg/L Kin gave the highest number 14.95 shoot/explant in multiplication stage and gave 16.90 shoot/explant for white cv., no significant differences between cultivars from culture node of the two cultivars at the multiplication stage highest shoots number 6.07 shoot/explant were obtained from the node culture on MS medium at 2.0 mg/L Kin and the highest shoot number 12.70 shoot were obtained from shoot tip culture of yellow cv. on MS medium supplemented with 8.0 mg/L Kin, shoots which produced in vitro for white cv. gave the highest rooting 50% with the highest root number 1.75 root/shoot when culture on half strength MS medium supplemented with 1.5 mg /L IAA . All the plantlets which produced in vitro acclimatized in laboratory then transferred to the greenhouse to grow normally.https://magrj.mosuljournals.com/article_33632_0e0c6eb0c33b9e2675a944113149539f.pdf
spellingShingle Basher Z. A. Kassab Bashy
EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
Mesopotamia Journal of Agriculture
title EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
title_full EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
title_fullStr EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
title_full_unstemmed EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
title_short EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO
title_sort eeeect of kinetin on initiation and multiplication of shoot tips and nodes of carnation in vitro
url https://magrj.mosuljournals.com/article_33632_0e0c6eb0c33b9e2675a944113149539f.pdf
work_keys_str_mv AT basherzakassabbashy eeeectofkinetinoninitiationandmultiplicationofshoottipsandnodesofcarnationinvitro