Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia
Background. This study described various molecular and epidemiological characters determining antibiotic resistance patterns in Pseudomonas aeruginosa isolates. Methods. A total of 34 carbapenem-resistant P. aeruginosa clinical isolates were isolated from samples collected at a tertiary hospital in...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/4379686 |
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| author | Mohamed H. Al-Agamy Katy Jeannot Taghrid S. El-Mahdy Hassan A. Samaha Atef M. Shibl Patrick Plésiat Patrice Courvalin |
| author_facet | Mohamed H. Al-Agamy Katy Jeannot Taghrid S. El-Mahdy Hassan A. Samaha Atef M. Shibl Patrick Plésiat Patrice Courvalin |
| author_sort | Mohamed H. Al-Agamy |
| collection | DOAJ |
| description | Background. This study described various molecular and epidemiological characters determining antibiotic resistance patterns in Pseudomonas aeruginosa isolates. Methods. A total of 34 carbapenem-resistant P. aeruginosa clinical isolates were isolated from samples collected at a tertiary hospital in Riyadh, Saudi Arabia, from January to December 2011. Susceptibility testing, serotyping, molecular characterization of carbapenem resistance, and pulsed-field gel electrophoresis (PFGE) were performed. Results. All isolates were resistant to ceftazidime, and more than half were highly resistant (minimum inhibitory concentration (MIC) > 256 mg/L). Fifteen isolates had MIC values ≥64 mg/L for any of the carbapenems examined. Vietnamese extended-spectrum β-lactamase (VEB-1) (n=16/34) and oxacillinase (OXA-10) (n=14/34) were the most prevalent extended-spectrum β-lactamase and penicillinase, respectively. Verona imipenemase (VIM-1, VIM-2, VIM-4, VIM-11, and VIM-28) and imipenemase (IMP-7) variants were found in metallo-β-lactamase producers. A decrease in outer membrane porin gene (oprD) expression was seen in nine isolates, and an increase in efflux pump gene (MexAB) expression was detected in five isolates. Six serotypes (O:1, O:4, O:7, O:10, O:11, and O:15) were found among the 34 isolates. The predominant serotype was O:11 (16 isolates), followed by O:15 (nine isolates). PFGE analysis of the 34 carbapenem-resistant P. aeruginosa isolates revealed 14 different pulsotypes. Conclusions. These results revealed diverse mechanisms conferring carbapenem resistance to P. aeruginosa isolates from Saudi Arabia. |
| format | Article |
| id | doaj-art-4e24dfa2cc874cda85faf09ae23c2329 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-4e24dfa2cc874cda85faf09ae23c23292025-08-20T02:38:46ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932016-01-01201610.1155/2016/43796864379686Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi ArabiaMohamed H. Al-Agamy0Katy Jeannot1Taghrid S. El-Mahdy2Hassan A. Samaha3Atef M. Shibl4Patrick Plésiat5Patrice Courvalin6Pharmaceutics Department, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaLaboratoire de Bactériologie, Centre National de Référence de la Résistance aux Antibiotiques, Besançon, FranceMicrobiology and Immunology Department, Faculty of Pharmacy, Helwan University, Cairo, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, EgyptPharmaceutics Department, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaLaboratoire de Bactériologie, Centre National de Référence de la Résistance aux Antibiotiques, Besançon, FranceUnité des Agents Antimicrobiens, Institut Pasteur, Paris, FranceBackground. This study described various molecular and epidemiological characters determining antibiotic resistance patterns in Pseudomonas aeruginosa isolates. Methods. A total of 34 carbapenem-resistant P. aeruginosa clinical isolates were isolated from samples collected at a tertiary hospital in Riyadh, Saudi Arabia, from January to December 2011. Susceptibility testing, serotyping, molecular characterization of carbapenem resistance, and pulsed-field gel electrophoresis (PFGE) were performed. Results. All isolates were resistant to ceftazidime, and more than half were highly resistant (minimum inhibitory concentration (MIC) > 256 mg/L). Fifteen isolates had MIC values ≥64 mg/L for any of the carbapenems examined. Vietnamese extended-spectrum β-lactamase (VEB-1) (n=16/34) and oxacillinase (OXA-10) (n=14/34) were the most prevalent extended-spectrum β-lactamase and penicillinase, respectively. Verona imipenemase (VIM-1, VIM-2, VIM-4, VIM-11, and VIM-28) and imipenemase (IMP-7) variants were found in metallo-β-lactamase producers. A decrease in outer membrane porin gene (oprD) expression was seen in nine isolates, and an increase in efflux pump gene (MexAB) expression was detected in five isolates. Six serotypes (O:1, O:4, O:7, O:10, O:11, and O:15) were found among the 34 isolates. The predominant serotype was O:11 (16 isolates), followed by O:15 (nine isolates). PFGE analysis of the 34 carbapenem-resistant P. aeruginosa isolates revealed 14 different pulsotypes. Conclusions. These results revealed diverse mechanisms conferring carbapenem resistance to P. aeruginosa isolates from Saudi Arabia.http://dx.doi.org/10.1155/2016/4379686 |
| spellingShingle | Mohamed H. Al-Agamy Katy Jeannot Taghrid S. El-Mahdy Hassan A. Samaha Atef M. Shibl Patrick Plésiat Patrice Courvalin Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia |
| title_full | Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia |
| title_fullStr | Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia |
| title_full_unstemmed | Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia |
| title_short | Diversity of Molecular Mechanisms Conferring Carbapenem Resistance to Pseudomonas aeruginosa Isolates from Saudi Arabia |
| title_sort | diversity of molecular mechanisms conferring carbapenem resistance to pseudomonas aeruginosa isolates from saudi arabia |
| url | http://dx.doi.org/10.1155/2016/4379686 |
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