Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats

Abstract Cerebral blood flow (CBF) appears to be selectively distributed in vital brain regions during rapid severe hypotension caused by tachyarrhythmia. To examine the effect of cardiopulmonary vagal afferent signals on region-specific CBF regulation, we compared CBF responses 1–4 s from the onset...

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Main Authors: Kei Ishii, Hidehiko Komine, Takeyuki Oba
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-05406-1
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author Kei Ishii
Hidehiko Komine
Takeyuki Oba
author_facet Kei Ishii
Hidehiko Komine
Takeyuki Oba
author_sort Kei Ishii
collection DOAJ
description Abstract Cerebral blood flow (CBF) appears to be selectively distributed in vital brain regions during rapid severe hypotension caused by tachyarrhythmia. To examine the effect of cardiopulmonary vagal afferent signals on region-specific CBF regulation, we compared CBF responses 1–4 s from the onset of ventricular pacing (at 550–850 beats/min) between vagotomized and sham-operated anesthetized male Wistar rats. Laser Doppler flowmetry was used to measure CBF in nine different brain regions from the cortex to the pons. The effects of vagotomy on cerebrovascular responsiveness to 5% CO2 exposure were also examined. Vagotomized rats showed an increased baseline heart rate and a diminished decrease in mean arterial pressure (MAP) during ventricular pacing compared with the sham-operated rats. In all the brain regions, CBF responses to pacing-induced moderate (− 34 mmHg ≤ ΔMAP ≤  − 15 mmHg) and severe hypotension (− 54 mmHg ≤ ΔMAP ≤  − 35 mmHg) were comparable between the groups. Vagotomy had no effect on the cerebrovascular response time or reactivity to hypercapnia. The present findings suggest that cardiopulmonary vagal afferent signals are not essential for cerebrovascular counterregulation immediately after the onset of ventricular tachycardia.
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spelling doaj-art-4e1845d490b74192a1df9de4abbec9be2025-08-20T03:45:23ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-05406-1Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized ratsKei Ishii0Hidehiko Komine1Takeyuki Oba2Human Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and TechnologyHuman Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and TechnologyHuman Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and TechnologyAbstract Cerebral blood flow (CBF) appears to be selectively distributed in vital brain regions during rapid severe hypotension caused by tachyarrhythmia. To examine the effect of cardiopulmonary vagal afferent signals on region-specific CBF regulation, we compared CBF responses 1–4 s from the onset of ventricular pacing (at 550–850 beats/min) between vagotomized and sham-operated anesthetized male Wistar rats. Laser Doppler flowmetry was used to measure CBF in nine different brain regions from the cortex to the pons. The effects of vagotomy on cerebrovascular responsiveness to 5% CO2 exposure were also examined. Vagotomized rats showed an increased baseline heart rate and a diminished decrease in mean arterial pressure (MAP) during ventricular pacing compared with the sham-operated rats. In all the brain regions, CBF responses to pacing-induced moderate (− 34 mmHg ≤ ΔMAP ≤  − 15 mmHg) and severe hypotension (− 54 mmHg ≤ ΔMAP ≤  − 35 mmHg) were comparable between the groups. Vagotomy had no effect on the cerebrovascular response time or reactivity to hypercapnia. The present findings suggest that cardiopulmonary vagal afferent signals are not essential for cerebrovascular counterregulation immediately after the onset of ventricular tachycardia.https://doi.org/10.1038/s41598-025-05406-1Cerebral blood flowVagotomyCardiopulmonary afferentsVentricular tachycardia
spellingShingle Kei Ishii
Hidehiko Komine
Takeyuki Oba
Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
Scientific Reports
Cerebral blood flow
Vagotomy
Cardiopulmonary afferents
Ventricular tachycardia
title Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
title_full Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
title_fullStr Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
title_full_unstemmed Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
title_short Effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
title_sort effects of vagal denervation on cerebral blood flow regulation immediately after the onset of ventricular pacing in anesthetized rats
topic Cerebral blood flow
Vagotomy
Cardiopulmonary afferents
Ventricular tachycardia
url https://doi.org/10.1038/s41598-025-05406-1
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AT hidehikokomine effectsofvagaldenervationoncerebralbloodflowregulationimmediatelyaftertheonsetofventricularpacinginanesthetizedrats
AT takeyukioba effectsofvagaldenervationoncerebralbloodflowregulationimmediatelyaftertheonsetofventricularpacinginanesthetizedrats