Assessment of testicular corticosterone biosynthesis in adult male rats.
Corticosterone is synthesized in the adrenal glands and is circulated throughout the body to perform regulatory functions in various tissues. The testis is known to synthesize and secrete testosterone and other androgens. We developed an accurate method to measure steroid content using liquid chroma...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117795&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850191027876397056 |
|---|---|
| author | Naoyuki Maeda Sachi Tahata Takeshi Yagi Emi Tanaka Kanae Masu Michiko Sato Satoko Haeno Takenori Onaga Hiroshi Yokota |
| author_facet | Naoyuki Maeda Sachi Tahata Takeshi Yagi Emi Tanaka Kanae Masu Michiko Sato Satoko Haeno Takenori Onaga Hiroshi Yokota |
| author_sort | Naoyuki Maeda |
| collection | DOAJ |
| description | Corticosterone is synthesized in the adrenal glands and is circulated throughout the body to perform regulatory functions in various tissues. The testis is known to synthesize and secrete testosterone and other androgens. We developed an accurate method to measure steroid content using liquid chromatography-mass spectrometry analysis. In the present study, significant levels of the precursor compounds of testosterone and corticosterone synthesis could be detected in rat testis using this method. After adrenalectomy, corticosterone remained in the blood and testicular tissue at approximately 1% of the amount present in the control testis. When the excised testicular tissue was washed and incubated with NADH, NADPH and progesterone, not only testosterone and its precursors but also 11-deoxycorticosterone and corticosterone were produced; the levels of 11-deoxycorticosterone and corticosterone increased with incubation time. The production rate of 11-deoxycorticosterone from progesterone was estimated to be approximately 1/20 that of 17-hydroxyprogesterone, and the corticosterone level was approximately 1/10 that of testosterone. These ratios coincided with those in the testicular tissue of the adrenalectomized rats, indicating that corticosterone was synthesized in the testis and not in the blood. A primary finding of this study was that corticosterone and testosterone were synthesized in a 1/10-20 ratio in the testis. It is concluded that corticosterone, which has various functions, such as the regulation of glycolysis and mediating spermatogenesis, is produced locally in the testis and that this the local production is convenient and functional to respond to local needs. |
| format | Article |
| id | doaj-art-4e15e0806d994d2590560e757e7bf532 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4e15e0806d994d2590560e757e7bf5322025-08-20T02:15:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011779510.1371/journal.pone.0117795Assessment of testicular corticosterone biosynthesis in adult male rats.Naoyuki MaedaSachi TahataTakeshi YagiEmi TanakaKanae MasuMichiko SatoSatoko HaenoTakenori OnagaHiroshi YokotaCorticosterone is synthesized in the adrenal glands and is circulated throughout the body to perform regulatory functions in various tissues. The testis is known to synthesize and secrete testosterone and other androgens. We developed an accurate method to measure steroid content using liquid chromatography-mass spectrometry analysis. In the present study, significant levels of the precursor compounds of testosterone and corticosterone synthesis could be detected in rat testis using this method. After adrenalectomy, corticosterone remained in the blood and testicular tissue at approximately 1% of the amount present in the control testis. When the excised testicular tissue was washed and incubated with NADH, NADPH and progesterone, not only testosterone and its precursors but also 11-deoxycorticosterone and corticosterone were produced; the levels of 11-deoxycorticosterone and corticosterone increased with incubation time. The production rate of 11-deoxycorticosterone from progesterone was estimated to be approximately 1/20 that of 17-hydroxyprogesterone, and the corticosterone level was approximately 1/10 that of testosterone. These ratios coincided with those in the testicular tissue of the adrenalectomized rats, indicating that corticosterone was synthesized in the testis and not in the blood. A primary finding of this study was that corticosterone and testosterone were synthesized in a 1/10-20 ratio in the testis. It is concluded that corticosterone, which has various functions, such as the regulation of glycolysis and mediating spermatogenesis, is produced locally in the testis and that this the local production is convenient and functional to respond to local needs.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117795&type=printable |
| spellingShingle | Naoyuki Maeda Sachi Tahata Takeshi Yagi Emi Tanaka Kanae Masu Michiko Sato Satoko Haeno Takenori Onaga Hiroshi Yokota Assessment of testicular corticosterone biosynthesis in adult male rats. PLoS ONE |
| title | Assessment of testicular corticosterone biosynthesis in adult male rats. |
| title_full | Assessment of testicular corticosterone biosynthesis in adult male rats. |
| title_fullStr | Assessment of testicular corticosterone biosynthesis in adult male rats. |
| title_full_unstemmed | Assessment of testicular corticosterone biosynthesis in adult male rats. |
| title_short | Assessment of testicular corticosterone biosynthesis in adult male rats. |
| title_sort | assessment of testicular corticosterone biosynthesis in adult male rats |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117795&type=printable |
| work_keys_str_mv | AT naoyukimaeda assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT sachitahata assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT takeshiyagi assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT emitanaka assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT kanaemasu assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT michikosato assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT satokohaeno assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT takenorionaga assessmentoftesticularcorticosteronebiosynthesisinadultmalerats AT hiroshiyokota assessmentoftesticularcorticosteronebiosynthesisinadultmalerats |