Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling
A variety of ligands differ in their capacity to bind the receptor, elicit gene expression, and modulate physiological responses. Such receptors include Toll-like receptors (TLRs), which recognize various patterns of pathogens and lead to primary innate immune activation against invaders, and G-prot...
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BioMed Central
2012-09-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-10-153.pdf |
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| author | Jayalakshmi Krishnan Sangdun Choi |
| author_facet | Jayalakshmi Krishnan Sangdun Choi |
| author_sort | Jayalakshmi Krishnan |
| collection | DOAJ |
| description | A variety of ligands differ in their capacity to bind the receptor, elicit gene expression, and modulate physiological responses. Such receptors include Toll-like receptors (TLRs), which recognize various patterns of pathogens and lead to primary innate immune activation against invaders, and G-protein coupled receptors (GPCRs), whose interaction with their cognate ligands activates heterotrimeric G proteins and regulates specific downstream effectors, including immuno-stimulating molecules. Once TLRs are activated, they lead to the expression of hundreds of genes together and bridge the arm of innate and adaptive immune responses. We characterized the gene expression profile of Toll-like receptor 4 (TLR4) in RAW 264.7 cells when it bound with its ligand, 2-keto-3-deoxyoctonate (KDO), the active part of lipopolysaccharide. In addition, to determine the network communications among the TLR, Janus kinase (JAK)/signal transducer and activator of transcription (STAT), and GPCR, we tested RAW 264.7 cells with KDO, interferon-β, or cAMP analog 8-Br. The ligands were also administered as a pair of double and triple combinations. |
| format | Article |
| id | doaj-art-4e0ab15cb61a4a428ed37372e6e26220 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-4e0ab15cb61a4a428ed37372e6e262202025-02-02T04:29:26ZengBioMed CentralGenomics & Informatics1598-866X2234-07422012-09-0110315316610.5808/GI.2012.10.3.1539Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR SignalingJayalakshmi Krishnan0Sangdun Choi1Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.A variety of ligands differ in their capacity to bind the receptor, elicit gene expression, and modulate physiological responses. Such receptors include Toll-like receptors (TLRs), which recognize various patterns of pathogens and lead to primary innate immune activation against invaders, and G-protein coupled receptors (GPCRs), whose interaction with their cognate ligands activates heterotrimeric G proteins and regulates specific downstream effectors, including immuno-stimulating molecules. Once TLRs are activated, they lead to the expression of hundreds of genes together and bridge the arm of innate and adaptive immune responses. We characterized the gene expression profile of Toll-like receptor 4 (TLR4) in RAW 264.7 cells when it bound with its ligand, 2-keto-3-deoxyoctonate (KDO), the active part of lipopolysaccharide. In addition, to determine the network communications among the TLR, Janus kinase (JAK)/signal transducer and activator of transcription (STAT), and GPCR, we tested RAW 264.7 cells with KDO, interferon-β, or cAMP analog 8-Br. The ligands were also administered as a pair of double and triple combinations.http://genominfo.org/upload/pdf/gni-10-153.pdfgene expressionG-protein coupled receptorsimmune responsesinnate immunitymacrophagesToll-like receptors |
| spellingShingle | Jayalakshmi Krishnan Sangdun Choi Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling Genomics & Informatics gene expression G-protein coupled receptors immune responses innate immunity macrophages Toll-like receptors |
| title | Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling |
| title_full | Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling |
| title_fullStr | Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling |
| title_full_unstemmed | Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling |
| title_short | Systems Biological Approaches Reveal Non-additive Responses and Multiple Crosstalk Mechanisms between TLR and GPCR Signaling |
| title_sort | systems biological approaches reveal non additive responses and multiple crosstalk mechanisms between tlr and gpcr signaling |
| topic | gene expression G-protein coupled receptors immune responses innate immunity macrophages Toll-like receptors |
| url | http://genominfo.org/upload/pdf/gni-10-153.pdf |
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