Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking
Sous vide, a cooking method that involves vacuum-sealed fish at low temperatures, yields a uniquely tender, easily flaked texture. Previous research on sous-vide tenderization has focused on thermal protein denaturation. On the other hand, the contribution of proteases, activated at low temperatures...
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MDPI AG
2024-11-01
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| author | Miyu Sakuyama Yuri Kominami Hideki Ushio |
| author_facet | Miyu Sakuyama Yuri Kominami Hideki Ushio |
| author_sort | Miyu Sakuyama |
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| description | Sous vide, a cooking method that involves vacuum-sealed fish at low temperatures, yields a uniquely tender, easily flaked texture. Previous research on sous-vide tenderization has focused on thermal protein denaturation. On the other hand, the contribution of proteases, activated at low temperatures in fish meat, has been suggested. However, the details of protein degradation remain unclear. This study employed SDS-PAGE/immunoblot and peptidomic analysis of rainbow trout to assess proteolysis during sous-vide cooking. The results from SDS-PAGE and immunoblot analysis indicated reduced thermal aggregation of sarcoplasmic proteins and increased depolymerization of actin under low-temperature cooking conditions. A comparison of the peptidome showed that the proteolysis of myofibrillar proteins was accelerated during sous-vide cooking, with distinct proteases potentially activated at different cooking temperatures. Terminome analysis revealed the contribution of specific proteases at higher temperatures in rainbow trout. The results of this study demonstrate the thermal denaturation of sarcoplasmic proteins and proteolysis of myofibrillar proteins in rainbow trout meat during sous-vide cooking and its temperature dependence. The methodology in the present study could provide insights into the optimization of cooking conditions for different fish species, potentially leading to improved texture and quality of sous-vide products. |
| format | Article |
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| institution | DOAJ |
| issn | 2227-7382 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Proteomes |
| spelling | doaj-art-4e088bdb92024d6ea72b2a5aa08fd2fd2025-08-20T02:43:45ZengMDPI AGProteomes2227-73822024-11-011243610.3390/proteomes12040036Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide CookingMiyu Sakuyama0Yuri Kominami1Hideki Ushio2Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-865, JapanDepartment of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-865, JapanDepartment of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-865, JapanSous vide, a cooking method that involves vacuum-sealed fish at low temperatures, yields a uniquely tender, easily flaked texture. Previous research on sous-vide tenderization has focused on thermal protein denaturation. On the other hand, the contribution of proteases, activated at low temperatures in fish meat, has been suggested. However, the details of protein degradation remain unclear. This study employed SDS-PAGE/immunoblot and peptidomic analysis of rainbow trout to assess proteolysis during sous-vide cooking. The results from SDS-PAGE and immunoblot analysis indicated reduced thermal aggregation of sarcoplasmic proteins and increased depolymerization of actin under low-temperature cooking conditions. A comparison of the peptidome showed that the proteolysis of myofibrillar proteins was accelerated during sous-vide cooking, with distinct proteases potentially activated at different cooking temperatures. Terminome analysis revealed the contribution of specific proteases at higher temperatures in rainbow trout. The results of this study demonstrate the thermal denaturation of sarcoplasmic proteins and proteolysis of myofibrillar proteins in rainbow trout meat during sous-vide cooking and its temperature dependence. The methodology in the present study could provide insights into the optimization of cooking conditions for different fish species, potentially leading to improved texture and quality of sous-vide products.https://www.mdpi.com/2227-7382/12/4/36sous-vide cookingproteolysispeptidometerminome |
| spellingShingle | Miyu Sakuyama Yuri Kominami Hideki Ushio Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking Proteomes sous-vide cooking proteolysis peptidome terminome |
| title | Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking |
| title_full | Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking |
| title_fullStr | Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking |
| title_full_unstemmed | Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking |
| title_short | Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Meat During Sous-Vide Cooking |
| title_sort | peptidomic analysis reveals temperature dependent proteolysis in rainbow trout i oncorhynchus mykiss i meat during sous vide cooking |
| topic | sous-vide cooking proteolysis peptidome terminome |
| url | https://www.mdpi.com/2227-7382/12/4/36 |
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