In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae
Verticillium dahliae is highly prone to pathogenic differentiation and influenced by host cotton's resistance. To better understand the mechanisms of this phenomenon, we applied the host selective pressures of resistant and susceptible cotton varieties on V. dahliae strain Vd076 within an artif...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000661 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856896814088192 |
|---|---|
| author | Yalin Zhang Lifang Cui Ruibing Liu Zili Feng Hongjie Feng Jinglong Zhou Lihong Zhao Feng Wei Heqin Zhu |
| author_facet | Yalin Zhang Lifang Cui Ruibing Liu Zili Feng Hongjie Feng Jinglong Zhou Lihong Zhao Feng Wei Heqin Zhu |
| author_sort | Yalin Zhang |
| collection | DOAJ |
| description | Verticillium dahliae is highly prone to pathogenic differentiation and influenced by host cotton's resistance. To better understand the mechanisms of this phenomenon, we applied the host selective pressures of resistant and susceptible cotton varieties on V. dahliae strain Vd076 within an artificial cotton Verticillium wilt nursery and greenhouse. Consequently, among the offspring strains, high virulence strains exhibited higher levels of physiological characteristics and genetic diversity compared to moderate and low virulence strains. Moreover, whole genome resequencing revealed that the Ka/Ks ratio of single nucleotide polymorphism (SNPs) in the majority of the offspring strains was about 0.6, indicating an adverse selection impact in the offspring strains. Pathogenicity assays demonstrated that the virulence of the offspring strains triggered by continuous induction of disease-resistant cotton cultivar increased from the 4th generation and reached its peak by the 6th generation. Additionally, the transcriptome analysis revealed that the 4th and 6th generations of strains differentially expressed genes (DEGs) accumulated a significant number of response genes associated with pathogen pathogenicity differentiation, including the mitogen-activated protein kinase (MAPK) signaling pathway, amino and antibiotic biosynthesis, phenylpropanoid metabolism. Furthermore, VDAG_04757, VDAG_06462, VDAG_03218, and VDAG_08487 genes exhibited significant correlation with the pathogenicity of V. dahliae. Collectively, this study has significant implications for elucidating the evolution of virulence in V. dahliae induced by the host, as well as for advancing holistic strategies for preventing and managing Verticillium wilt disease. |
| format | Article |
| id | doaj-art-4dfc16dd5ea84530bbce3f89ee334ecc |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-4dfc16dd5ea84530bbce3f89ee334ecc2025-02-12T05:30:07ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117730In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliaeYalin Zhang0Lifang Cui1Ruibing Liu2Zili Feng3Hongjie Feng4Jinglong Zhou5Lihong Zhao6Feng Wei7Heqin Zhu8State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China; Western Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji 831100, China; Hebei Agricultural University, Hebei Research Base of State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China; Western Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji 831100, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China; Corresponding author.State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China; Western Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji 831100, China; Corresponding author at: State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.Verticillium dahliae is highly prone to pathogenic differentiation and influenced by host cotton's resistance. To better understand the mechanisms of this phenomenon, we applied the host selective pressures of resistant and susceptible cotton varieties on V. dahliae strain Vd076 within an artificial cotton Verticillium wilt nursery and greenhouse. Consequently, among the offspring strains, high virulence strains exhibited higher levels of physiological characteristics and genetic diversity compared to moderate and low virulence strains. Moreover, whole genome resequencing revealed that the Ka/Ks ratio of single nucleotide polymorphism (SNPs) in the majority of the offspring strains was about 0.6, indicating an adverse selection impact in the offspring strains. Pathogenicity assays demonstrated that the virulence of the offspring strains triggered by continuous induction of disease-resistant cotton cultivar increased from the 4th generation and reached its peak by the 6th generation. Additionally, the transcriptome analysis revealed that the 4th and 6th generations of strains differentially expressed genes (DEGs) accumulated a significant number of response genes associated with pathogen pathogenicity differentiation, including the mitogen-activated protein kinase (MAPK) signaling pathway, amino and antibiotic biosynthesis, phenylpropanoid metabolism. Furthermore, VDAG_04757, VDAG_06462, VDAG_03218, and VDAG_08487 genes exhibited significant correlation with the pathogenicity of V. dahliae. Collectively, this study has significant implications for elucidating the evolution of virulence in V. dahliae induced by the host, as well as for advancing holistic strategies for preventing and managing Verticillium wilt disease.http://www.sciencedirect.com/science/article/pii/S0147651325000661CottonHost-pathogen interactionPathogenicity geneVerticillium dahliaeVirulence variation |
| spellingShingle | Yalin Zhang Lifang Cui Ruibing Liu Zili Feng Hongjie Feng Jinglong Zhou Lihong Zhao Feng Wei Heqin Zhu In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae Ecotoxicology and Environmental Safety Cotton Host-pathogen interaction Pathogenicity gene Verticillium dahliae Virulence variation |
| title | In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae |
| title_full | In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae |
| title_fullStr | In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae |
| title_full_unstemmed | In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae |
| title_short | In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae |
| title_sort | in the coevolution of cotton and pathogenic fungi resistant cotton varieties lead to an escalation in the virulence of verticillium dahliae |
| topic | Cotton Host-pathogen interaction Pathogenicity gene Verticillium dahliae Virulence variation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000661 |
| work_keys_str_mv | AT yalinzhang inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT lifangcui inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT ruibingliu inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT zilifeng inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT hongjiefeng inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT jinglongzhou inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT lihongzhao inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT fengwei inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae AT heqinzhu inthecoevolutionofcottonandpathogenicfungiresistantcottonvarietiesleadtoanescalationinthevirulenceofverticilliumdahliae |