Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems
Hantaviruses, including the Sin Nombre virus (SNV) and Andes virus (ANDV), are associated with severe global health risks, causing high mortality rates in hantavirus pulmonary syndrome (HPS) patients. Neutralizing antibodies are essential for virus clearance and survival, making neutralization assay...
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MDPI AG
2025-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/5/723 |
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| author | Punya Shrivastava-Ranjan Jamie A. Kelly Laura K. McMullan Deborah Cannon Laura Morgan Payel Chatterjee Shilpi Jain Joel M. Montgomery Mike Flint César G. Albariño Christina F. Spiropoulou |
| author_facet | Punya Shrivastava-Ranjan Jamie A. Kelly Laura K. McMullan Deborah Cannon Laura Morgan Payel Chatterjee Shilpi Jain Joel M. Montgomery Mike Flint César G. Albariño Christina F. Spiropoulou |
| author_sort | Punya Shrivastava-Ranjan |
| collection | DOAJ |
| description | Hantaviruses, including the Sin Nombre virus (SNV) and Andes virus (ANDV), are associated with severe global health risks, causing high mortality rates in hantavirus pulmonary syndrome (HPS) patients. Neutralizing antibodies are essential for virus clearance and survival, making neutralization assays critical for understanding immunity and evaluating therapeutic strategies. In this study, we developed a recombinant vesicular stomatitis virus (VSV)-based surrogate system expressing SNV and ANDV glycoproteins (GPCs), enabling neutralization studies under biosafety level 2 conditions. The neutralization titers obtained with the VSV-based system closely matched the findings from authentic hantavirus assays performed under biosafety level 3 conditions, confirming its potential as a useful tool for determining immune responses and advancing hantavirus research. |
| format | Article |
| id | doaj-art-4df379be22f1435d89994beb990c610f |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-4df379be22f1435d89994beb990c610f2025-08-20T03:47:57ZengMDPI AGViruses1999-49152025-05-0117572310.3390/v17050723Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus SystemsPunya Shrivastava-Ranjan0Jamie A. Kelly1Laura K. McMullan2Deborah Cannon3Laura Morgan4Payel Chatterjee5Shilpi Jain6Joel M. Montgomery7Mike Flint8César G. Albariño9Christina F. Spiropoulou10Viral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAHantaviruses, including the Sin Nombre virus (SNV) and Andes virus (ANDV), are associated with severe global health risks, causing high mortality rates in hantavirus pulmonary syndrome (HPS) patients. Neutralizing antibodies are essential for virus clearance and survival, making neutralization assays critical for understanding immunity and evaluating therapeutic strategies. In this study, we developed a recombinant vesicular stomatitis virus (VSV)-based surrogate system expressing SNV and ANDV glycoproteins (GPCs), enabling neutralization studies under biosafety level 2 conditions. The neutralization titers obtained with the VSV-based system closely matched the findings from authentic hantavirus assays performed under biosafety level 3 conditions, confirming its potential as a useful tool for determining immune responses and advancing hantavirus research.https://www.mdpi.com/1999-4915/17/5/723hantaviridaeorthohantavirusSin Nombre virusAndes virushemorrhagic fever |
| spellingShingle | Punya Shrivastava-Ranjan Jamie A. Kelly Laura K. McMullan Deborah Cannon Laura Morgan Payel Chatterjee Shilpi Jain Joel M. Montgomery Mike Flint César G. Albariño Christina F. Spiropoulou Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems Viruses hantaviridae orthohantavirus Sin Nombre virus Andes virus hemorrhagic fever |
| title | Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems |
| title_full | Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems |
| title_fullStr | Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems |
| title_full_unstemmed | Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems |
| title_short | Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems |
| title_sort | evaluating neutralizing antibodies in hantavirus infected patients using authentic virus and recombinant vesicular stomatitis virus systems |
| topic | hantaviridae orthohantavirus Sin Nombre virus Andes virus hemorrhagic fever |
| url | https://www.mdpi.com/1999-4915/17/5/723 |
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