Direct 3D Mass Spectrometry Imaging Analysis of Environmental Microorganisms

Direct 3D Mass Spectrometry Imaging Analysis of Environmental Microorganisms

Assessing the spatial distribution of microorganisms’ metabolites in growth medium remains a challenge. Here, we present the first use of the newly developed LARAPPI/CI-MSI 3D (laser ablation remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging) method for direct...

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Bibliographic Details
Main Authors: Justyna Szulc, Tomasz Grzyb, Joanna Nizioł, Sumi Krupa, Wiktoria Szuberla, Tomasz Ruman
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Molecules
Subjects:
2D/3D microbiological culture imaging
mass spectrometry imaging
microbial interactions
phytopathogens
bioactive compounds
metabolomic analysis
Online Access:https://www.mdpi.com/1420-3049/30/6/1317
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Summary:Assessing the spatial distribution of microorganisms’ metabolites in growth medium remains a challenge. Here, we present the first use of the newly developed LARAPPI/CI-MSI 3D (laser ablation remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging) method for direct three-dimensional (3D) mass spectrometry imaging of bacterial and fungal metabolites in solid culture media. Two-dimensional (2D) MSI was also performed, and it indicated the presence of metabolites belonging to, and including, amino acids and their derivatives, dipeptides, organic acids, fatty acids, sugars and sugar derivatives, benzene derivatives, and indoles. Distribution at a selected depth within the culture medium with the estimation of concentration across all dimensions of 16 metabolites was visualized using LARAPPI/CI-MSI 3D. The imaging results were correlated with the results of ultra-high-performance liquid chromatography–ultra-high-resolution mass spectrometry (UHPLC–UHRMS). A total of 351–393 chemical compounds, depending on the tested microorganism, were identified, while 242–262 were recognized in the HMDB database in MetaboAnalyst (v 6.0). The LARAPPI/CI-MSI 3D method enables the rapid screening of the biotechnological potential of environmental strains, facilitating the discovery of industrially valuable biomolecules.
ISSN:1420-3049

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