A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection
Simple, cost-effective, sensitive, and specific approaches that are capable of enabling point-of-care testing (POCT) are crucial for clinical diagnosis, especially in settings with extremely limited resources. Therefore, a universal, simple, low cost, sensitive, and specific POCT approach called RCA...
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| Language: | English |
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Elsevier
2025-12-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053925000815 |
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| author | Xiaopeng Chen Wentao Wang Pin Zhang Qionglin Wang Kangbo Liu Fengfang Ding Xianwei Zhang Ligong Hou Yingyu Zhang Wancun Zhang |
| author_facet | Xiaopeng Chen Wentao Wang Pin Zhang Qionglin Wang Kangbo Liu Fengfang Ding Xianwei Zhang Ligong Hou Yingyu Zhang Wancun Zhang |
| author_sort | Xiaopeng Chen |
| collection | DOAJ |
| description | Simple, cost-effective, sensitive, and specific approaches that are capable of enabling point-of-care testing (POCT) are crucial for clinical diagnosis, especially in settings with extremely limited resources. Therefore, a universal, simple, low cost, sensitive, and specific POCT approach called RCA-F-LFS was developed by combining molecular beacon (MB)-assisted rolling circle amplification (RCA) for target amplification with fluorescent lateral flow strips (F-LFS) to enable highly sensitive and visual detection of RCA products. Using Mycoplasma pneumoniae as the detection target, RCA-F-LFS demonstrated high sensitivity (0.1 pM) and specificity, accurately distinguishing it from other pathogenic microorganisms. In addition, RCA-F-LFS enables visual detection of both Mycoplasma pneumoniae DNA and RNA. The detection results of clinical throat swab samples showed that RCA-F-LFS could accurately detect Mycoplasma pneumoniae, with 100 % agreement with current clinical approaches. As a proof of concept, RCA-F-LFS can detect other nucleic acid targets by simply changing the target sequence, offering a robust and versatile strategy for the development of novel clinical POCT detection methods. |
| format | Article |
| id | doaj-art-4debb56e2f7f40ecbb3c463ce3dd7827 |
| institution | DOAJ |
| issn | 2666-0539 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj-art-4debb56e2f7f40ecbb3c463ce3dd78272025-08-20T03:08:55ZengElsevierSensors and Actuators Reports2666-05392025-12-011010036310.1016/j.snr.2025.100363A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detectionXiaopeng Chen0Wentao Wang1Pin Zhang2Qionglin Wang3Kangbo Liu4Fengfang Ding5Xianwei Zhang6Ligong Hou7Yingyu Zhang8Wancun Zhang9Henan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR ChinaHenan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR ChinaHenan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR ChinaHenan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR ChinaNMPA Key Laboratory for Quality Evaluation of Medical Protective and Implant Devices, Henan Institute for Drug and Medical Device Inspection (Henan Vaccine Issuance Center), Zhengzhou 450018, PR ChinaZhengzhou Information Engineering Vocational College, Zhengzhou 450100, PR ChinaHealth Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR ChinaHenan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR China; Corresponding authors.Henan Key Laboratory of Rare Diseases, Endocrinology and Metabolism Center, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, PR China; Corresponding authors.Henan International Joint Laboratory for Prevention and Treatment of Pediatric Disease, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR China; Health Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, PR China; Corresponding authors.Simple, cost-effective, sensitive, and specific approaches that are capable of enabling point-of-care testing (POCT) are crucial for clinical diagnosis, especially in settings with extremely limited resources. Therefore, a universal, simple, low cost, sensitive, and specific POCT approach called RCA-F-LFS was developed by combining molecular beacon (MB)-assisted rolling circle amplification (RCA) for target amplification with fluorescent lateral flow strips (F-LFS) to enable highly sensitive and visual detection of RCA products. Using Mycoplasma pneumoniae as the detection target, RCA-F-LFS demonstrated high sensitivity (0.1 pM) and specificity, accurately distinguishing it from other pathogenic microorganisms. In addition, RCA-F-LFS enables visual detection of both Mycoplasma pneumoniae DNA and RNA. The detection results of clinical throat swab samples showed that RCA-F-LFS could accurately detect Mycoplasma pneumoniae, with 100 % agreement with current clinical approaches. As a proof of concept, RCA-F-LFS can detect other nucleic acid targets by simply changing the target sequence, offering a robust and versatile strategy for the development of novel clinical POCT detection methods.http://www.sciencedirect.com/science/article/pii/S2666053925000815Rolling circle amplificationFluorescent lateral flow stripsPoint-of-care testingNucleic acid detectionUniversality |
| spellingShingle | Xiaopeng Chen Wentao Wang Pin Zhang Qionglin Wang Kangbo Liu Fengfang Ding Xianwei Zhang Ligong Hou Yingyu Zhang Wancun Zhang A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection Sensors and Actuators Reports Rolling circle amplification Fluorescent lateral flow strips Point-of-care testing Nucleic acid detection Universality |
| title | A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection |
| title_full | A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection |
| title_fullStr | A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection |
| title_full_unstemmed | A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection |
| title_short | A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection |
| title_sort | universal fluorescent lateral flow strip approach based on molecular beacon assisted rolling circle amplification for point of care nucleic acid detection |
| topic | Rolling circle amplification Fluorescent lateral flow strips Point-of-care testing Nucleic acid detection Universality |
| url | http://www.sciencedirect.com/science/article/pii/S2666053925000815 |
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