Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2
Mice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were co...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2007-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.03.0237 |
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| _version_ | 1850052399376367616 |
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| author | XIE Rong-hui WAN Wang-jun LI Long LI Jian-rong YU Lian |
| author_facet | XIE Rong-hui WAN Wang-jun LI Long LI Jian-rong YU Lian |
| author_sort | XIE Rong-hui |
| collection | DOAJ |
| description | Mice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were coadministrated orally and intradermally with DNA vaccine and ZJ111/pCI-IL-2 at three dosage levels (10<sup>7</sup> cfu, 10<sup>8</sup> cfu,10<sup>9</sup> cfu), and boosted two weeks later. Control groups (challenge control, normal control, DNA vaccine control) were included. The chickens were challenged with virulent infectious bursal disease virus (IBDV) seventeen days post-boosting. The results showed that protective responses and neutralization antibody response of DNA vaccine co-administrated with chicken interleukin-2 (IL-2) were much higher than those of injected with DNA vaccine alone. Futhermore, the T lymphocyte proliferation response of thymus and spleen, and the B lymphocyte proliferation response of bursa induced by DNA vaccine can be significantly enhanced by chicken IL-2. These results obviously indicated that chicken IL-2 was a strong adjuvant can be significantly enhance the immunogenicity of IBDV DNA vaccine. |
| format | Article |
| id | doaj-art-4de904facd674984bacedb177f3569ae |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-4de904facd674984bacedb177f3569ae2025-08-20T02:52:49ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-05-013323724210.3785/1008-9209.2007.03.023710089209Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2XIE Rong-huiWAN Wang-junLI LongLI Jian-rongYU LianMice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were coadministrated orally and intradermally with DNA vaccine and ZJ111/pCI-IL-2 at three dosage levels (10<sup>7</sup> cfu, 10<sup>8</sup> cfu,10<sup>9</sup> cfu), and boosted two weeks later. Control groups (challenge control, normal control, DNA vaccine control) were included. The chickens were challenged with virulent infectious bursal disease virus (IBDV) seventeen days post-boosting. The results showed that protective responses and neutralization antibody response of DNA vaccine co-administrated with chicken interleukin-2 (IL-2) were much higher than those of injected with DNA vaccine alone. Futhermore, the T lymphocyte proliferation response of thymus and spleen, and the B lymphocyte proliferation response of bursa induced by DNA vaccine can be significantly enhanced by chicken IL-2. These results obviously indicated that chicken IL-2 was a strong adjuvant can be significantly enhance the immunogenicity of IBDV DNA vaccine.https://www.academax.com/doi/10.3785/1008-9209.2007.03.0237chicken interleukin-2 (IL-2)infectious bursal disease virus (IBDV)immunity enhancing |
| spellingShingle | XIE Rong-hui WAN Wang-jun LI Long LI Jian-rong YU Lian Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 浙江大学学报. 农业与生命科学版 chicken interleukin-2 (IL-2) infectious bursal disease virus (IBDV) immunity enhancing |
| title | Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
| title_full | Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
| title_fullStr | Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
| title_full_unstemmed | Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
| title_short | Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
| title_sort | enhancement of immunogenicity of dna vaccine against infectious bursal disease virus by co delivery with oral vaccine encoding chicken interleukin 2 |
| topic | chicken interleukin-2 (IL-2) infectious bursal disease virus (IBDV) immunity enhancing |
| url | https://www.academax.com/doi/10.3785/1008-9209.2007.03.0237 |
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