Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2
Mice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were co...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.03.0237 |
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| Summary: | Mice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were coadministrated orally and intradermally with DNA vaccine and ZJ111/pCI-IL-2 at three dosage levels (10<sup>7</sup> cfu, 10<sup>8</sup> cfu,10<sup>9</sup> cfu), and boosted two weeks later. Control groups (challenge control, normal control, DNA vaccine control) were included. The chickens were challenged with virulent infectious bursal disease virus (IBDV) seventeen days post-boosting. The results showed that protective responses and neutralization antibody response of DNA vaccine co-administrated with chicken interleukin-2 (IL-2) were much higher than those of injected with DNA vaccine alone. Futhermore, the T lymphocyte proliferation response of thymus and spleen, and the B lymphocyte proliferation response of bursa induced by DNA vaccine can be significantly enhanced by chicken IL-2. These results obviously indicated that chicken IL-2 was a strong adjuvant can be significantly enhance the immunogenicity of IBDV DNA vaccine. |
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| ISSN: | 1008-9209 2097-5155 |