Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China
In 2022, a significant PRV outbreak in a southwestern China pig farm led to a high incidence of sow abortion. A serological analysis using <i>gE</i> antigen-based ELISA revealed a high prevalence (69.30%) of PRV <i>gE</i> antibodies among the affected pigs, with a significant...
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MDPI AG
2024-10-01
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| author | Jiaqi Wu Juan Zhang Jun Zhou Yi Luo Xinrong Wang Rui Yang Junhai Zhu Meiyu Jia Longxiang Zhang Lizhi Fu Nan Yan Yue Wang |
| author_facet | Jiaqi Wu Juan Zhang Jun Zhou Yi Luo Xinrong Wang Rui Yang Junhai Zhu Meiyu Jia Longxiang Zhang Lizhi Fu Nan Yan Yue Wang |
| author_sort | Jiaqi Wu |
| collection | DOAJ |
| description | In 2022, a significant PRV outbreak in a southwestern China pig farm led to a high incidence of sow abortion. A serological analysis using <i>gE</i> antigen-based ELISA revealed a high prevalence (69.30%) of PRV <i>gE</i> antibodies among the affected pigs, with a significant variation across different pig populations (1.11–76.12%). We collected additional 5552 pig serum samples and 580 pig cerebrospinal fluid (CSF) samples from various pig farms in Southwest China between 2022 and 2024. The seropositive rates for PRV <i>gE</i> antibodies ranged from 2.36% and 8.65% in the serum samples, while the positive detection rates for the PRV <i>gE</i> gene in the cerebrospinal fluid samples, as determined by PCR, were between 1.06% and 2.36%. The PCR analysis and sequencing of the PRV <i>gB</i>, <i>gC</i>, <i>gE</i>, and <i>TK</i> genes from eight randomly selected samples identified two distinct strains, CQ1 and CQ2. CQ1’s <i>gC</i> gene showed similarity to the vaccine strain Bartha, while the other genes aligned with Chinese classical strains, suggesting its potential genetic recombination. CQ2 aligned with the Chinese classical strain SC. Although the overall PRV infection in Southwest China’s pig farms is relatively low, occasional outbreaks with high positivity rates are observed. These findings highlight the necessity for increased surveillance and stringent control measures to safeguard the swine industry. |
| format | Article |
| id | doaj-art-4de68b2f6e9548e0a677ca821dc9377b |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-4de68b2f6e9548e0a677ca821dc9377b2025-08-20T02:14:15ZengMDPI AGAnimals2076-26152024-10-011421310310.3390/ani14213103Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest ChinaJiaqi Wu0Juan Zhang1Jun Zhou2Yi Luo3Xinrong Wang4Rui Yang5Junhai Zhu6Meiyu Jia7Longxiang Zhang8Lizhi Fu9Nan Yan10Yue Wang11College of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaSichuan Boce Testing Technology Co., Ltd., Chengdu 610023, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaChongqing Academy of Animal Science, Chongqing 408599, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaChongqing Academy of Animal Science, Chongqing 408599, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 400715, ChinaIn 2022, a significant PRV outbreak in a southwestern China pig farm led to a high incidence of sow abortion. A serological analysis using <i>gE</i> antigen-based ELISA revealed a high prevalence (69.30%) of PRV <i>gE</i> antibodies among the affected pigs, with a significant variation across different pig populations (1.11–76.12%). We collected additional 5552 pig serum samples and 580 pig cerebrospinal fluid (CSF) samples from various pig farms in Southwest China between 2022 and 2024. The seropositive rates for PRV <i>gE</i> antibodies ranged from 2.36% and 8.65% in the serum samples, while the positive detection rates for the PRV <i>gE</i> gene in the cerebrospinal fluid samples, as determined by PCR, were between 1.06% and 2.36%. The PCR analysis and sequencing of the PRV <i>gB</i>, <i>gC</i>, <i>gE</i>, and <i>TK</i> genes from eight randomly selected samples identified two distinct strains, CQ1 and CQ2. CQ1’s <i>gC</i> gene showed similarity to the vaccine strain Bartha, while the other genes aligned with Chinese classical strains, suggesting its potential genetic recombination. CQ2 aligned with the Chinese classical strain SC. Although the overall PRV infection in Southwest China’s pig farms is relatively low, occasional outbreaks with high positivity rates are observed. These findings highlight the necessity for increased surveillance and stringent control measures to safeguard the swine industry.https://www.mdpi.com/2076-2615/14/21/3103pseudorabies virusepidemiological surveygenetic variation |
| spellingShingle | Jiaqi Wu Juan Zhang Jun Zhou Yi Luo Xinrong Wang Rui Yang Junhai Zhu Meiyu Jia Longxiang Zhang Lizhi Fu Nan Yan Yue Wang Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China Animals pseudorabies virus epidemiological survey genetic variation |
| title | Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China |
| title_full | Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China |
| title_fullStr | Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China |
| title_full_unstemmed | Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China |
| title_short | Prevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China |
| title_sort | prevalence and genetic variation investigation of the pseudorabies virus in southwest china |
| topic | pseudorabies virus epidemiological survey genetic variation |
| url | https://www.mdpi.com/2076-2615/14/21/3103 |
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