Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation

To address the challenges in modeling and optimization caused by nonlinear dynamic coupling and real-time measurement difficulties of key biological parameters in <i>Pichia pastoris</i> fermentation processes, this study proposes a soft-sensing method based on Adam-Fully Connected Neural...

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Main Authors: Bo Wang, Wenyu Ma, Hui Jiang, Shaowen Huang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Sensors
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Online Access:https://www.mdpi.com/1424-8220/25/13/4105
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author Bo Wang
Wenyu Ma
Hui Jiang
Shaowen Huang
author_facet Bo Wang
Wenyu Ma
Hui Jiang
Shaowen Huang
author_sort Bo Wang
collection DOAJ
description To address the challenges in modeling and optimization caused by nonlinear dynamic coupling and real-time measurement difficulties of key biological parameters in <i>Pichia pastoris</i> fermentation processes, this study proposes a soft-sensing method based on Adam-Fully Connected Neural Network inverse. Firstly, a non-deterministic mechanism model is constructed to characterize the dynamic coupling relationships among multiple variables in the fermentation process, and the reversibility of the system and the construction method of the inverse extended model are analyzed. Further, by leveraging the nonlinear fitting capabilities of the Fully Connected Neural Network to identify the inverse extended model, an adaptive learning rate optimization algorithm is introduced to dynamically adjust the learning rate of the Fully Connected Neural Network, thereby enhancing the convergence and robustness of the nonlinear system. Finally, a composite pseudo-linear system is formed by cascading the inverse model with the original system, achieving decoupling and the high-accuracy prediction of key parameters. Experimental results demonstrate that the proposed method significantly reduces prediction errors and enhances generalization capabilities compared to traditional models, validating the effectiveness of the proposed method in complex bioprocesses.
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spelling doaj-art-4de51d4216da47ff8beffff5468bc81f2025-08-20T02:36:30ZengMDPI AGSensors1424-82202025-06-012513410510.3390/s25134105Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> FermentationBo Wang0Wenyu Ma1Hui Jiang2Shaowen Huang3School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, ChinaTo address the challenges in modeling and optimization caused by nonlinear dynamic coupling and real-time measurement difficulties of key biological parameters in <i>Pichia pastoris</i> fermentation processes, this study proposes a soft-sensing method based on Adam-Fully Connected Neural Network inverse. Firstly, a non-deterministic mechanism model is constructed to characterize the dynamic coupling relationships among multiple variables in the fermentation process, and the reversibility of the system and the construction method of the inverse extended model are analyzed. Further, by leveraging the nonlinear fitting capabilities of the Fully Connected Neural Network to identify the inverse extended model, an adaptive learning rate optimization algorithm is introduced to dynamically adjust the learning rate of the Fully Connected Neural Network, thereby enhancing the convergence and robustness of the nonlinear system. Finally, a composite pseudo-linear system is formed by cascading the inverse model with the original system, achieving decoupling and the high-accuracy prediction of key parameters. Experimental results demonstrate that the proposed method significantly reduces prediction errors and enhances generalization capabilities compared to traditional models, validating the effectiveness of the proposed method in complex bioprocesses.https://www.mdpi.com/1424-8220/25/13/4105Adam optimization<i>Pichia pastoris</i>FCNNfermentationsoft-sensing
spellingShingle Bo Wang
Wenyu Ma
Hui Jiang
Shaowen Huang
Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
Sensors
Adam optimization
<i>Pichia pastoris</i>
FCNN
fermentation
soft-sensing
title Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
title_full Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
title_fullStr Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
title_full_unstemmed Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
title_short Research on Soft-Sensing Method Based on Adam-FCNN Inversion in <i>Pichia pastoris</i> Fermentation
title_sort research on soft sensing method based on adam fcnn inversion in i pichia pastoris i fermentation
topic Adam optimization
<i>Pichia pastoris</i>
FCNN
fermentation
soft-sensing
url https://www.mdpi.com/1424-8220/25/13/4105
work_keys_str_mv AT bowang researchonsoftsensingmethodbasedonadamfcnninversioninipichiapastorisifermentation
AT wenyuma researchonsoftsensingmethodbasedonadamfcnninversioninipichiapastorisifermentation
AT huijiang researchonsoftsensingmethodbasedonadamfcnninversioninipichiapastorisifermentation
AT shaowenhuang researchonsoftsensingmethodbasedonadamfcnninversioninipichiapastorisifermentation