First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome
These datasets correspond to sequencing of DNA and RNA extracted from surface-disinfected teliospores of the fungus Thecaphora frezzii Carranza and Lindquist, causal agent of smut disease in peanut (Arachis hypogaea L.). The DNA was sequenced using Pacific Biosciences (PacBio) Sequel II and RNA was...
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Elsevier
2025-08-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340925005062 |
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| author | Renee S. Arias John T. Dobbs Valerie A. Orner E. Cinthia Conforto Alejandro M. Rago Luis I. Cazon Victor S. Sobolev Imana L. Power Marshall C. Lamb Alicia N. Massa |
| author_facet | Renee S. Arias John T. Dobbs Valerie A. Orner E. Cinthia Conforto Alejandro M. Rago Luis I. Cazon Victor S. Sobolev Imana L. Power Marshall C. Lamb Alicia N. Massa |
| author_sort | Renee S. Arias |
| collection | DOAJ |
| description | These datasets correspond to sequencing of DNA and RNA extracted from surface-disinfected teliospores of the fungus Thecaphora frezzii Carranza and Lindquist, causal agent of smut disease in peanut (Arachis hypogaea L.). The DNA was sequenced using Pacific Biosciences (PacBio) Sequel II and RNA was sequenced in Illumina MiSeq 300 bp paired end (PE). Initial de novo assembly and Basic Local Alignment Search Tool (BLAST) of contigs to the 16S reference sequence database at NCBI had hits on seven bacterial species: Luteibacter pinisoli, Variovorax paradoxus, Rhizobium metallidurans, Caulobacter segnis, Roseateles violae, Novosphingobium rosa, Herbaspirillum seropedicae, and the fungus T. frezzii. High stringency mapping of the RNA sequences to the genomes of these organisms showed that 96% of the reads corresponded to bacteria and only 4% to T. frezzii. De novo assembly of PacBio reads revealed the whole genome of a Luteibacter sp. with 88.56% or lower similarity to those in NCBI database, whereas most RNA sequences (> 10 million reads) mapped to the new Luteibacter sp. genome assembled here. We annotated the new Luteibacter sp. genome, assessed its completeness by BUSCO, and aligned it to its closest relative, Luteibacter aegosomatissinici. We are not aware of any report that describes bacteria colonizing teliospores of T. frezzii. The DNA and RNA sequencing datasets provided here could be used to study the distribution of prokaryotes colonizing T. frezzii teliospores, and to explore the role of the microbiome of T. frezzii teliospores on the outcome of peanut smut disease. |
| format | Article |
| id | doaj-art-4de33cb0984d497fa04edca5b7314cc5 |
| institution | DOAJ |
| issn | 2352-3409 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj-art-4de33cb0984d497fa04edca5b7314cc52025-08-20T03:18:38ZengElsevierData in Brief2352-34092025-08-016111177910.1016/j.dib.2025.111779First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genomeRenee S. Arias0John T. Dobbs1Valerie A. Orner2E. Cinthia Conforto3Alejandro M. Rago4Luis I. Cazon5Victor S. Sobolev6Imana L. Power7Marshall C. Lamb8Alicia N. Massa9United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S.E., Dawson GA 39842, USA; Corresponding author.USDA-ARS, Foreign Disease-Weed Science Research, 1301 Ditto Avenue, Fort Detrick, MD 21702, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S.E., Dawson GA 39842, USAInstituto Nacional de Tecnologia Agropecuaria (INTA), Centro de Investigaciones Agropecuarias (CIAP), Instituto de Pathología Vegetal (IPAVE). Unidad de Fitopatología y Modelización Agrícola (UFyMA), CONICET. Calle 11 de septiembre 4755, 5020, Córdoba, ArgentinaInstituto Nacional de Tecnologia Agropecuaria (INTA), Centro de Investigaciones Agropecuarias (CIAP), Instituto de Pathología Vegetal (IPAVE). Unidad de Fitopatología y Modelización Agrícola (UFyMA), CONICET. Calle 11 de septiembre 4755, 5020, Córdoba, ArgentinaInstituto Nacional de Tecnologia Agropecuaria (INTA), Centro de Investigaciones Agropecuarias (CIAP), Instituto de Pathología Vegetal (IPAVE). Unidad de Fitopatología y Modelización Agrícola (UFyMA), CONICET. Calle 11 de septiembre 4755, 5020, Córdoba, ArgentinaUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S.E., Dawson GA 39842, USALouisiana State University, Dept. Plant Pathology & Crop Physiology, 302 Life Sciences Building, LSU Campus, Baton Rouge, LA 70803, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S.E., Dawson GA 39842, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S.E., Dawson GA 39842, USAThese datasets correspond to sequencing of DNA and RNA extracted from surface-disinfected teliospores of the fungus Thecaphora frezzii Carranza and Lindquist, causal agent of smut disease in peanut (Arachis hypogaea L.). The DNA was sequenced using Pacific Biosciences (PacBio) Sequel II and RNA was sequenced in Illumina MiSeq 300 bp paired end (PE). Initial de novo assembly and Basic Local Alignment Search Tool (BLAST) of contigs to the 16S reference sequence database at NCBI had hits on seven bacterial species: Luteibacter pinisoli, Variovorax paradoxus, Rhizobium metallidurans, Caulobacter segnis, Roseateles violae, Novosphingobium rosa, Herbaspirillum seropedicae, and the fungus T. frezzii. High stringency mapping of the RNA sequences to the genomes of these organisms showed that 96% of the reads corresponded to bacteria and only 4% to T. frezzii. De novo assembly of PacBio reads revealed the whole genome of a Luteibacter sp. with 88.56% or lower similarity to those in NCBI database, whereas most RNA sequences (> 10 million reads) mapped to the new Luteibacter sp. genome assembled here. We annotated the new Luteibacter sp. genome, assessed its completeness by BUSCO, and aligned it to its closest relative, Luteibacter aegosomatissinici. We are not aware of any report that describes bacteria colonizing teliospores of T. frezzii. The DNA and RNA sequencing datasets provided here could be used to study the distribution of prokaryotes colonizing T. frezzii teliospores, and to explore the role of the microbiome of T. frezzii teliospores on the outcome of peanut smut disease.http://www.sciencedirect.com/science/article/pii/S2352340925005062Arachis hypogaeaMicrobiomePeanut smutPeanutGroundnutLuteibacter |
| spellingShingle | Renee S. Arias John T. Dobbs Valerie A. Orner E. Cinthia Conforto Alejandro M. Rago Luis I. Cazon Victor S. Sobolev Imana L. Power Marshall C. Lamb Alicia N. Massa First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome Data in Brief Arachis hypogaea Microbiome Peanut smut Peanut Groundnut Luteibacter |
| title | First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome |
| title_full | First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome |
| title_fullStr | First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome |
| title_full_unstemmed | First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome |
| title_short | First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome |
| title_sort | first metagenome and metatranscriptome dataset of thecaphora frezzii teliospores assembly and annotation of a new bacterial genome |
| topic | Arachis hypogaea Microbiome Peanut smut Peanut Groundnut Luteibacter |
| url | http://www.sciencedirect.com/science/article/pii/S2352340925005062 |
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