Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells
In the recurrence of cancer and autoimmune diseases, cellular senescence, a state where cells cease division, plays a pivotal role. Distinguishing the senescence requires a combination of multiple markers. Fluorescent dyes incorporation limits the imaging observation time due to the potential toxici...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Science and Technology of Advanced Materials: Methods |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/27660400.2025.2525061 |
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| author | Mazaya Najmina Nicholaus Kevin Tanjaya Satoshi Ishii Koichiro Uto |
| author_facet | Mazaya Najmina Nicholaus Kevin Tanjaya Satoshi Ishii Koichiro Uto |
| author_sort | Mazaya Najmina |
| collection | DOAJ |
| description | In the recurrence of cancer and autoimmune diseases, cellular senescence, a state where cells cease division, plays a pivotal role. Distinguishing the senescence requires a combination of multiple markers. Fluorescent dyes incorporation limits the imaging observation time due to the potential toxicity of those compounds over prolonged exposure, while colorimetric dye incorporation requires cell fixation, where cells are not in native state, and long staining time (≥4 hours). One of the distinct characteristics that distinguishes senescent cells from other state of cells is the impaired protein degradation ability by the lysosome, resulting on the protein accumulation. In the current work, we take advantage of protein accumulation to distinguish senescence cells from other cells. The protein concentration is visualized by recording the phase difference using the quantitative phase microscopy (QPM). Our live QPM observations on breast cancer cells confirm that senescent cells exhibit a higher refractive index compared to cells in normal growth and quiescent states, attributed to the accumulation of undigested lysosomal protein cargo. This heightened refractive index suggests an imbalance in protein turnover rates within senescent cells. These findings shed light on a potential label-free non-invasive approach for a prolonged monitoring of senescent cell dynamics. |
| format | Article |
| id | doaj-art-4de30a2127d8485cab8f44c9f43c7d7b |
| institution | DOAJ |
| issn | 2766-0400 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials: Methods |
| spelling | doaj-art-4de30a2127d8485cab8f44c9f43c7d7b2025-08-20T03:12:48ZengTaylor & Francis GroupScience and Technology of Advanced Materials: Methods2766-04002025-12-015110.1080/27660400.2025.2525061Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cellsMazaya Najmina0Nicholaus Kevin Tanjaya1Satoshi Ishii2Koichiro Uto3Research Center of Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, JapanSubprogram in Materials Science and Engineering Graduate School of Science and Technology, University of Tsukuba, Tsukuba, JapanSubprogram in Materials Science and Engineering Graduate School of Science and Technology, University of Tsukuba, Tsukuba, JapanResearch Center of Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, JapanIn the recurrence of cancer and autoimmune diseases, cellular senescence, a state where cells cease division, plays a pivotal role. Distinguishing the senescence requires a combination of multiple markers. Fluorescent dyes incorporation limits the imaging observation time due to the potential toxicity of those compounds over prolonged exposure, while colorimetric dye incorporation requires cell fixation, where cells are not in native state, and long staining time (≥4 hours). One of the distinct characteristics that distinguishes senescent cells from other state of cells is the impaired protein degradation ability by the lysosome, resulting on the protein accumulation. In the current work, we take advantage of protein accumulation to distinguish senescence cells from other cells. The protein concentration is visualized by recording the phase difference using the quantitative phase microscopy (QPM). Our live QPM observations on breast cancer cells confirm that senescent cells exhibit a higher refractive index compared to cells in normal growth and quiescent states, attributed to the accumulation of undigested lysosomal protein cargo. This heightened refractive index suggests an imbalance in protein turnover rates within senescent cells. These findings shed light on a potential label-free non-invasive approach for a prolonged monitoring of senescent cell dynamics.https://www.tandfonline.com/doi/10.1080/27660400.2025.2525061Growth arrestlabel-free detectionsenescencequantitative phase microscopeprotein concentration |
| spellingShingle | Mazaya Najmina Nicholaus Kevin Tanjaya Satoshi Ishii Koichiro Uto Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells Science and Technology of Advanced Materials: Methods Growth arrest label-free detection senescence quantitative phase microscope protein concentration |
| title | Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells |
| title_full | Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells |
| title_fullStr | Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells |
| title_full_unstemmed | Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells |
| title_short | Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells |
| title_sort | label free classification of growth arrested state of cells by quantitative phase microscopy in fixative treated cells |
| topic | Growth arrest label-free detection senescence quantitative phase microscope protein concentration |
| url | https://www.tandfonline.com/doi/10.1080/27660400.2025.2525061 |
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