Expression of Integrin Alpha10 Is Induced in Malignant Melanoma
Recently, integrin alpha10 was described as a collagen type II-binding integrin expressed mainly in chondrocytes. However, by array studies we detected integrin alpha10 also to be upregulated in malignant melanoma compared to primary melanocytes. Subsequent analysis of melanoma cell lines and melano...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2007/601497 |
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| _version_ | 1832554010727415808 |
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| author | Ann-Kathrin Wenke Christian Kjellman Evy Lundgren-Akerlund Christian Uhlmann Nikolas K. Haass Meenhard Herlyn Anja K. Bosserhoff |
| author_facet | Ann-Kathrin Wenke Christian Kjellman Evy Lundgren-Akerlund Christian Uhlmann Nikolas K. Haass Meenhard Herlyn Anja K. Bosserhoff |
| author_sort | Ann-Kathrin Wenke |
| collection | DOAJ |
| description | Recently, integrin alpha10 was described as a collagen type II-binding integrin expressed mainly in chondrocytes. However, by array studies we detected integrin alpha10 also to be upregulated in malignant melanoma compared to primary melanocytes. Subsequent analysis of melanoma cell lines and melanoma tumor samples confirmed this finding. Further, we demonstrated that expression of integrin alpha10 is controlled by AP-2 and Ets-1, two transcription factors known to be involved in melanoma development and progression. To investigate the functional relevance of integrin alpha10, expression was downregulated via stable antisense transfection. Proliferation assays and colony forming assays revealed no differences comparing antisense integrin alpha10 cell clones with control and wild type melanoma cells, respectively. However, antisense integrin alpha10 cell clones and Mel Im cells treated with an inhibitory antibody against integrin alpha10 showed a reduced migratory potential. |
| format | Article |
| id | doaj-art-4dd4fc2d4b884dfabbe3c27d5f8a3f62 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-4dd4fc2d4b884dfabbe3c27d5f8a3f622025-02-03T05:52:33ZengWileyCellular Oncology1570-58701875-86062007-01-0129537338610.1155/2007/601497Expression of Integrin Alpha10 Is Induced in Malignant MelanomaAnn-Kathrin Wenke0Christian Kjellman1Evy Lundgren-Akerlund2Christian Uhlmann3Nikolas K. Haass4Meenhard Herlyn5Anja K. Bosserhoff6Institute of Pathology, University Regensburg, 93053 Regensburg, GermanyCartela AB, Biomedical Center B12, SE-221 84 Lund, SwedenCartela AB, Biomedical Center B12, SE-221 84 Lund, SwedenInstitute of Pathology, University Regensburg, 93053 Regensburg, GermanyThe Wistar Institute, Philadelphia, PA, USAThe Wistar Institute, Philadelphia, PA, USAInstitute of Pathology, University Regensburg, 93053 Regensburg, GermanyRecently, integrin alpha10 was described as a collagen type II-binding integrin expressed mainly in chondrocytes. However, by array studies we detected integrin alpha10 also to be upregulated in malignant melanoma compared to primary melanocytes. Subsequent analysis of melanoma cell lines and melanoma tumor samples confirmed this finding. Further, we demonstrated that expression of integrin alpha10 is controlled by AP-2 and Ets-1, two transcription factors known to be involved in melanoma development and progression. To investigate the functional relevance of integrin alpha10, expression was downregulated via stable antisense transfection. Proliferation assays and colony forming assays revealed no differences comparing antisense integrin alpha10 cell clones with control and wild type melanoma cells, respectively. However, antisense integrin alpha10 cell clones and Mel Im cells treated with an inhibitory antibody against integrin alpha10 showed a reduced migratory potential.http://dx.doi.org/10.1155/2007/601497 |
| spellingShingle | Ann-Kathrin Wenke Christian Kjellman Evy Lundgren-Akerlund Christian Uhlmann Nikolas K. Haass Meenhard Herlyn Anja K. Bosserhoff Expression of Integrin Alpha10 Is Induced in Malignant Melanoma Cellular Oncology |
| title | Expression of Integrin Alpha10 Is Induced in Malignant Melanoma |
| title_full | Expression of Integrin Alpha10 Is Induced in Malignant Melanoma |
| title_fullStr | Expression of Integrin Alpha10 Is Induced in Malignant Melanoma |
| title_full_unstemmed | Expression of Integrin Alpha10 Is Induced in Malignant Melanoma |
| title_short | Expression of Integrin Alpha10 Is Induced in Malignant Melanoma |
| title_sort | expression of integrin alpha10 is induced in malignant melanoma |
| url | http://dx.doi.org/10.1155/2007/601497 |
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