Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics

Background Deficiency of fluid and blood is the main clinical manifestation of middle- and advanced-stage patients with esophageal cancer, but there are few reports about the metabolomic characteristics of esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid an...

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Main Author: ZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing
Format: Article
Language:zho
Published: Chinese General Practice Publishing House Co., Ltd 2025-04-01
Series:Zhongguo quanke yixue
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Online Access:https://www.chinagp.net/fileup/1007-9572/PDF/1738829454266-1739236019.pdf
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author ZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing
author_facet ZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing
author_sort ZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing
collection DOAJ
description Background Deficiency of fluid and blood is the main clinical manifestation of middle- and advanced-stage patients with esophageal cancer, but there are few reports about the metabolomic characteristics of esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood. Objective To investigate the metabolomic characteristics of esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood. Methods From April to December 2022, 35 esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood, were selected as the case group in the departments of TCM and Thoracic Surgery, the Fourth Hospital of Hebei Medical University, meanwhile 35 healthy volunteers were selected as the control group. Serum specimens were collected, and metabolomic characteristics was analyzed by ultra performance liquid chromatography-mass spectrometry, and then screened the differential metabolites metabolic pathways. Results A total of 88 differential metabolites with substance secondary matching names were screened out; 5 key metabolic pathways with significant difference and 11 related differential metabolites were further screened out. The 5 key metabolic pathways were β-Alanine metabolism pathway, Sphingolipid metabolism pathway, Linoleic acid metabolism pathway, Glycine, serine and threonine metabolism pathway, and Arachidonic acid metabolism pathway. Of the 11 related differential metabolites, β-Alanine, Phosphatidylcholine, L-homoserine, and L-allothreonine were significantly down-regulated, whereas Carnosine, Linoleic acid, 9, 10-DHOME, Hydroxypyruvic acid, Arachidonic acid, Heparin B3, and Thromboxane B2 were significantly up-regulated. Conclusion There are metabolic pathways with significant difference and related differential metabolites in esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood, which mainly involved in metabolic abnormality of energy, such as amino acids and fatty acids.
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spelling doaj-art-4dd3238c2a8a4ae7b3a314df9a69a4332025-08-20T03:12:58ZzhoChinese General Practice Publishing House Co., LtdZhongguo quanke yixue1007-95722025-04-0128121513151910.12114/j.issn.1007-9572.2023.0542Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target MetabolomicsZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing01. Department of Traditional Chinese Medicine, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China;2. College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaBackground Deficiency of fluid and blood is the main clinical manifestation of middle- and advanced-stage patients with esophageal cancer, but there are few reports about the metabolomic characteristics of esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood. Objective To investigate the metabolomic characteristics of esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood. Methods From April to December 2022, 35 esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood, were selected as the case group in the departments of TCM and Thoracic Surgery, the Fourth Hospital of Hebei Medical University, meanwhile 35 healthy volunteers were selected as the control group. Serum specimens were collected, and metabolomic characteristics was analyzed by ultra performance liquid chromatography-mass spectrometry, and then screened the differential metabolites metabolic pathways. Results A total of 88 differential metabolites with substance secondary matching names were screened out; 5 key metabolic pathways with significant difference and 11 related differential metabolites were further screened out. The 5 key metabolic pathways were β-Alanine metabolism pathway, Sphingolipid metabolism pathway, Linoleic acid metabolism pathway, Glycine, serine and threonine metabolism pathway, and Arachidonic acid metabolism pathway. Of the 11 related differential metabolites, β-Alanine, Phosphatidylcholine, L-homoserine, and L-allothreonine were significantly down-regulated, whereas Carnosine, Linoleic acid, 9, 10-DHOME, Hydroxypyruvic acid, Arachidonic acid, Heparin B3, and Thromboxane B2 were significantly up-regulated. Conclusion There are metabolic pathways with significant difference and related differential metabolites in esophageal squamous cell carcinoma patients with TCM differentiation of deficiency of fluid and blood, which mainly involved in metabolic abnormality of energy, such as amino acids and fatty acids.https://www.chinagp.net/fileup/1007-9572/PDF/1738829454266-1739236019.pdfesophageal neoplasms|esophageal squamous cell carcinoma|metabolomics|deficiency of fluid and blood|chromatography, liquid
spellingShingle ZHANG Yushuang, WU Zhongbing, HUANG Ming, JIA Lei, GAO Shuang, ZHAO Weipeng, LI Jing
Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
Zhongguo quanke yixue
esophageal neoplasms|esophageal squamous cell carcinoma|metabolomics|deficiency of fluid and blood|chromatography, liquid
title Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
title_full Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
title_fullStr Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
title_full_unstemmed Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
title_short Study on Metabolic Characteristics in Esophageal Squamous Cell Carcinoma Patients with TCM Differentiation of Deficiency of Fluid and Blood Based on Non Target Metabolomics
title_sort study on metabolic characteristics in esophageal squamous cell carcinoma patients with tcm differentiation of deficiency of fluid and blood based on non target metabolomics
topic esophageal neoplasms|esophageal squamous cell carcinoma|metabolomics|deficiency of fluid and blood|chromatography, liquid
url https://www.chinagp.net/fileup/1007-9572/PDF/1738829454266-1739236019.pdf
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