Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms
This study aimed to determine the effects of low-temperature plasma jet produced in gas helium (LTP-helium) on cariogenic biofilms composedby Streptococcusmutans, Streptococcus sanguinis and Streptococcus gordonii, and also by the combination of Candida albicans, Lactobacillus acidophilus and S. mut...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2397831 |
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| author | Leandro Wagner Figueira Ana Bessa Muniz Anelise Cristina Osorio Cesar Doria Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Y. Koga-Ito |
| author_facet | Leandro Wagner Figueira Ana Bessa Muniz Anelise Cristina Osorio Cesar Doria Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Y. Koga-Ito |
| author_sort | Leandro Wagner Figueira |
| collection | DOAJ |
| description | This study aimed to determine the effects of low-temperature plasma jet produced in gas helium (LTP-helium) on cariogenic biofilms composedby Streptococcusmutans, Streptococcus sanguinis and Streptococcus gordonii, and also by the combination of Candida albicans, Lactobacillus acidophilus and S. mutans. Biofilms were treated for 1, 3, 5, and 7 minutes. A 0.12% chlorhexidine solution was used as the positive control and sterile physiologic solution was the negative control. Biofilm viability was analyzed by viable cell recovery, scanning electron microscopy, and confocal laser scanning microscopy. All assays were performed intriplicate in three independent experiments. Multispecies biofilms exposed to LTP-helium had a significant reduction in viability when compared to the negative control (p < 0.0001). For biofilm formedby S. mutans, S. sanguinis, and S. gordonii, LTP treatments for 5 and 7 minutes caused similar reduction of morethan 2 log10. Also, a significant reduction in the viability of biofilms formedby C. albicans, L. acidophilus, and S. mutans was detected (p < 0.0001). In conclusion, LTP-helium reduced theviability of cariogenic biofilms with different microbial compositions, which indicates that LTP-helium is a potential tool for developing new protocols for dental caries prevention and treatment. |
| format | Article |
| id | doaj-art-4dd29a6f29654cb191fae7bdcabacc9b |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-4dd29a6f29654cb191fae7bdcabacc9b2025-08-20T02:23:09ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2397831Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilmsLeandro Wagner Figueira0Ana Bessa Muniz1Anelise Cristina Osorio Cesar Doria2Thalita Mayumi Castaldelli Nishime3Konstantin Georgiev Kostov4Cristiane Y. Koga-Ito5Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University, UNESP, São José dos Campos, São Paulo, BrazilDepartment of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University, UNESP, São José dos Campos, São Paulo, BrazilBiotechnology and Electric Plasma Laboratory (Biotechplasma) - Research and Development Institute – IPD - Universidade Do Vale Do Paraíba. Av. Shishima Hifumi, São José dos Campos, BrazilLeibniz Institute for Plasma Science and Technology, Greifswald, GermanyDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University/ UNESP, Guaratinguetá, BrazilDepartment of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University, UNESP, São José dos Campos, São Paulo, BrazilThis study aimed to determine the effects of low-temperature plasma jet produced in gas helium (LTP-helium) on cariogenic biofilms composedby Streptococcusmutans, Streptococcus sanguinis and Streptococcus gordonii, and also by the combination of Candida albicans, Lactobacillus acidophilus and S. mutans. Biofilms were treated for 1, 3, 5, and 7 minutes. A 0.12% chlorhexidine solution was used as the positive control and sterile physiologic solution was the negative control. Biofilm viability was analyzed by viable cell recovery, scanning electron microscopy, and confocal laser scanning microscopy. All assays were performed intriplicate in three independent experiments. Multispecies biofilms exposed to LTP-helium had a significant reduction in viability when compared to the negative control (p < 0.0001). For biofilm formedby S. mutans, S. sanguinis, and S. gordonii, LTP treatments for 5 and 7 minutes caused similar reduction of morethan 2 log10. Also, a significant reduction in the viability of biofilms formedby C. albicans, L. acidophilus, and S. mutans was detected (p < 0.0001). In conclusion, LTP-helium reduced theviability of cariogenic biofilms with different microbial compositions, which indicates that LTP-helium is a potential tool for developing new protocols for dental caries prevention and treatment.https://www.tandfonline.com/doi/10.1080/20002297.2024.2397831Dental carieslow-temperature plasmacariogenic biofilm |
| spellingShingle | Leandro Wagner Figueira Ana Bessa Muniz Anelise Cristina Osorio Cesar Doria Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Y. Koga-Ito Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms Journal of Oral Microbiology Dental caries low-temperature plasma cariogenic biofilm |
| title | Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| title_full | Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| title_fullStr | Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| title_full_unstemmed | Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| title_short | Inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| title_sort | inhibitory effect of helium cold atmospheric plasma on cariogenic biofilms |
| topic | Dental caries low-temperature plasma cariogenic biofilm |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2397831 |
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